That binding relates to activity, and activity should be the same on both leaflets. Unless cholesterol is not at equilibrium between the leaflets, as nothing in biology seems to be.

So, are you suggesting that the difference in cholesterol amounts between each leaflet is caused by protein binding? If so, does this relate to changes in cholesterol accessibility when toxins like Perfringolysin O bind to the membrane?

And the hydrolysis of the peptide bond is spontaneous…

3/3 Hence, the use of the term "lipid raft" calls for a definition that is redundant given the already encompassing definition of lipid domains. #lipiddomain #membranes

2/3 Lipid domains appears when membranes phase separate. It is intuitive to understand that the properties of lipid domains will inevitably vary, based on the specific composition of the membrane in which they exist. #lipiddomain #membranes