Hey look who is online!

@tommy-letao-li.bsky.social

I will make Helen and Tommy get bluesky and then i will @ them in a subsequent post, but thank you for reading!!

This work was the last year or so of my Ph.D in the Bateup Lab as covid hit, and I didn’t have an opportunity to complete it.

Very fortunately, Tommy came along and added SO much (immunopanning, 4i, additional scRNA-seq, westerns and all the analysis) making it way better than i ever could have!

There is a ton of IF showing the remarkable pathology of both the knockout organoid astrocytes (left) and astrocytes from resected patient cortical tuber tissue

The single cell sequencing was performed at multiple important timepoints in early development and in multiple genetic backgrounds, all with our meticulously engineered conditional two-hit TSC2 knockout alleles and knockout reporter cassettes

Excited (again) to see this paper finally in (pre)print!

We did single-cell sequencing on our TSC model organoids and observed strong signatures of reactive astrogliosis (neat)- then we looked at resected patient brain tissue and saw the same thing! (More neat!)

www.biorxiv.org/content/10.1...

Also I couldn't have done it without the fellowship support of the @jcchildsfund.bsky.social - thank you!

This method is adaptable to many cell types and systems which we have tried internally - if you are interested in trying this out, please get in touch! Additionally, phospho-seq.com has more resources with protocols and data from each tested antibody, with more to come!

There are significant changes from the initial pre-print posted .... some time ago.... including profiling retinal organoids, adding new benchmarks and demonstrating an additional method to incorporate transcriptomic data

I had a lot of help with this paper and special thanks to my collaborators from the Treutlein Lab including @epign.bsky.social ! (see, some people ARE on here).

Happy that my first paper from my Postdoc is out where we perform Phospho-seq, a single-cell ATAC-sequencing with intracellular protein quantification in human brain organoids: www.nature.com/articles/s41...