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John Blair

@jblairsci.bsky.social

18 followers 12 following 11 posts

Postdoc in the Satija Lab at NYGC. Former PhD at Bateup Lab at UC Berkeley

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John Blair @jblairsci.bsky.social · Mar 9

Hey look who is online!

@tommy-letao-li.bsky.social

John Blair @jblairsci.bsky.social · Mar 6

I will make Helen and Tommy get bluesky and then i will @ them in a subsequent post, but thank you for reading!!

John Blair @jblairsci.bsky.social · Mar 6

This work was the last year or so of my Ph.D in the Bateup Lab as covid hit, and I didn’t have an opportunity to complete it.

Very fortunately, Tommy came along and added SO much (immunopanning, 4i, additional scRNA-seq, westerns and all the analysis) making it way better than i ever could have!

John Blair @jblairsci.bsky.social · Mar 6

There is a ton of IF showing the remarkable pathology of both the knockout organoid astrocytes (left) and astrocytes from resected patient cortical tuber tissue

John Blair @jblairsci.bsky.social · Mar 6

The single cell sequencing was performed at multiple important timepoints in early development and in multiple genetic backgrounds, all with our meticulously engineered conditional two-hit TSC2 knockout alleles and knockout reporter cassettes

John Blair @jblairsci.bsky.social · Mar 6

Excited (again) to see this paper finally in (pre)print!

We did single-cell sequencing on our TSC model organoids and observed strong signatures of reactive astrogliosis (neat)- then we looked at resected patient brain tissue and saw the same thing! (More neat!)

www.biorxiv.org/content/10.1...

mTORC1 activation drives astrocyte reactivity in cortical tubers and brain organoid models of TSC

Tuberous Sclerosis Complex (TSC) is a genetic neurodevelopmental disorder associated with early onset epilepsy, intellectual disability and neuropsychiatric disorders. A hallmark of the disorder is co...

www.biorxiv.org

John Blair @jblairsci.bsky.social · Feb 28

Also I couldn't have done it without the fellowship support of the @jcchildsfund.bsky.social - thank you!

John Blair @jblairsci.bsky.social · Feb 28

This method is adaptable to many cell types and systems which we have tried internally - if you are interested in trying this out, please get in touch! Additionally, phospho-seq.com has more resources with protocols and data from each tested antibody, with more to come!

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phospho-seq.com

John Blair @jblairsci.bsky.social · Feb 28

There are significant changes from the initial pre-print posted .... some time ago.... including profiling retinal organoids, adding new benchmarks and demonstrating an additional method to incorporate transcriptomic data

John Blair @jblairsci.bsky.social · Feb 28

I had a lot of help with this paper and special thanks to my collaborators from the Treutlein Lab including @epign.bsky.social ! (see, some people ARE on here).

John Blair @jblairsci.bsky.social · Feb 28

Happy that my first paper from my Postdoc is out where we perform Phospho-seq, a single-cell ATAC-sequencing with intracellular protein quantification in human brain organoids: www.nature.com/articles/s41...

Phospho-seq: integrated, multi-modal profiling of intracellular protein dynamics in single cells - Nature Communications

Here, the authors demonstrate Phospho-seq, a single-cell multiomics method capable of quantifying chromatin accessibility alongside intracellular proteins, including post-translationally modified prot...