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Jérémy Dufloo @jeremydufloo.bsky.social · Sep 4

Congratulations François !! 👏

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Jérémy Dufloo @jeremydufloo.bsky.social · Aug 23

Thanks Maria! 😊🙏🏻

Jérémy Dufloo @jeremydufloo.bsky.social · Aug 18

Happy to share the final version of our article on the identification and characterization of novel bat coronaviruses, published in PLOS Pathogens @plos.org🦇🎉 Congrats to Claudia, first author of this paper, and all co-authors! 👏

dx.plos.org/10.1371/jour...

Identification and characterization of novel bat coronaviruses in Spain

Author summary Bats carry many different viruses, some of which can infect humans. Among these, bat coronaviruses are of particular concern. To be better prepared for future pandemics, it is important...

dx.plos.org

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Jérémy Dufloo @jeremydufloo.bsky.social · Jun 19

Thrilled to have been awarded a Ramon y Cajal 5-year tenure-track contract to continue working on viral entry (and more!) with @rafa-sanjuan.bsky.social at @i2sysbio.es and @uv.es 😀🎉

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Reposted by Jérémy Dufloo

The Sato Lab (Kei Sato) @systemsvirology.bsky.social · Feb 12

BREAKING🔔 The 50th🎉 paper from G2P-Japan🇯🇵 is out at Lancet Infectious Diseases
@TheLancetInfDis. We elucidated the virological characteristics of SARS-CoV-2 LP.8.1. Please repost🔥
www.thelancet.com/journals/lan...

Virological characteristics of the SARS-CoV-2 LP.8.1 variant

After the rapid spread of the SARS-CoV-2 JN.1 (BA.2.86.1.1) variant,1 several descendant lineages of JN.1, including KP.2 (JN.1.11.1.2),2 KP.3 (JN.1.11.1.3),2 and KP.3.1.1 (JN.1.11.1.3.1.1),3 have eme...

www.thelancet.com

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Reposted by Jérémy Dufloo

The Rey Lab @virusfusion007.bsky.social · Jan 25

Check out our most recent preprint concerning the identification of a protein receptor for the coronavirus #PHEV.
Collaboration with collaboration that we had with @jeremydufloo.bsky.social and Sanjuán’ lab

Dipeptidase 1 is a functional receptor for coronavirus PHEV

Coronaviruses of the subgenus Embecovirus include several relevant pathogens such as the human seasonal coronaviruses HKU1 and OC43, bovine coronavirus, and porcine hemagglutinating encephalomyelitis ...

www.biorxiv.org

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 23

Press release from the University of Valencia about our recently published article! 📰🗞️

www.uv.es/uvweb/uv-new...

Human cells have few barriers to prevent the entry of animal viruses

A research team from the Institute of Integrative Systems Biology (I2SysBio), a joint centre of the University of Valencia and the Spanish National Research Council (CSIC), asserts that the proteins r...

www.uv.es

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 11

Thanks a lot Spyros! Glad you liked them! 🙏🏻☺️

Jérémy Dufloo @jeremydufloo.bsky.social · Jan 10

I'd like to warmly thank all the collaborators involved, especially in the Sanjuán and Rey labs, in particular Ignacio Fernández (not on Bluesky), co-first author of the study! Hope you'll enjoy the read! 📚

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 10

Altogether, this highlights the diversity in protein and glycan receptor usage among embecoviruses, and suggests that the embecovirus RBD is a highly versatile structure that can accommodate highly divergent sequences to achieve novel receptor specificities.

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 10

Finally, Ignacio used cryoEM to obtain the structure of the PHEV spike ectodomain. This revealed that in contrast to all other embecovirus spikes structurally resolved (OC43, HKU1, MHV), the PHEV spike spontaneously adopts open conformations, allowing interaction with DPEP1.

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 10

This allowed us to identify critical residues at the interface. Morevover, this revealed that despite variations in RBD sequences and receptor usage , the structural elements involved in receptor recognition are conserved across embecoviruses.

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 10

Ignacio Fernández, from Félix Rey's lab, beautifully obtained the X-ray structure of DPEP1 in complex with the PHEV RBD.

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 10

DPEP1 was a PHEV-specific receptor and could not bind other betacoronavirus 1 spikes. This could be explained by large sequence variations between the RBD of PHEV and that of other members of the species, including BCoV or OC43.

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 10

Here, we show that contrary to other embecoviruses, the entry of PHEV does not require binding to sialic acid. In contrast, we identify dipeptidase 1 (DPEP1) as a functional PHEV receptor triggering PHEV spike-mediated fusion (entry and cell-cell fusion).

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 10

Therefore, sialic acid had long been considered as the only embecovirus receptor. However, the recent discovery that TMPRSS2 acts as the protein receptor of HKU1 revealed a more complex scenario. Whether other embecovirus dually depends on a protein and sialic acid is unknown.

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 10

The N-terminal domain (NTD) of the embecovirus spike harbors a conserved glycan-binding site that interacts with sialic acid in HKU1, OC43, BCoV, and PHEV and this interaction is necessary for HKU1, OC43 and BCoV entry.

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 10

The betacoronavirus 1 species is the main taxon of the Embecovirus subgenus (Betacoronavirus genus), which includes other relevant viruses such as the human common cold virus HKU1 🤒 and murine hepatitis virus (MHV) 🐁.

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 10

In this work, we focused on the betacoronavirus 1 species. It includes ten host-specific viruses such as the porcine hemagglutinating encephalomyelitis virus (PHEV) 🐖 and bovine coronavirus (BCoV) 🐄, but also the human seasonal cold virus OC43 🤒.

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 10

🚨 Preprint alter - bis 🚨

I am thrilled to share our last preprint where we identify and characterize a new coronavirus receptor! 🦠

This work was done in collaboration with the team of Félix Rey at the Institut Pasteur.

A 🧵 below...

www.biorxiv.org/content/10.1...

Dipeptidase 1 is a functional receptor for coronavirus PHEV

Coronaviruses of the subgenus Embecovirus include several relevant pathogens such as the human seasonal coronaviruses HKU1 and OC43, bovine coronavirus, and porcine hemagglutinating encephalomyelitis ...

www.biorxiv.org

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 9

🚨 New preprint alert 🚨

We searched for the presence of coronaviruses in >200 bat individuals across Spain 🦠🦇. We recovered 6 complete coronavirus genomes, including 3 novel species. One of them could use bat and human ACE2 for entry.

www.biorxiv.org/content/10.1...

Identification and characterization of novel bat coronaviruses in Spain

The zoonotic transmission of bat coronaviruses poses a threat to human health. However, the diversity of bat-borne coronaviruses remains poorly characterized in many geographical areas. Here, we recov...

www.biorxiv.org

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 6

Thanks! 🙏🏻

Jérémy Dufloo @jeremydufloo.bsky.social · Jan 2

I'd like to warmly thank everyone in Rafael Sanjuán's lab involved in this project! 🙏😃 I hope you'll enjoy the read, and don't hesitate to reach out if you're interested in any of the pseudotypes we generated! 😉

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 2

Altogether, our results suggest that post-entry stages of the infection cycle, as well as epidemiological and ecological factors, may be more critical determinants of viral zoonosis than entry.

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Jérémy Dufloo @jeremydufloo.bsky.social · Jan 2

We also tested the role of non-protein factors (i.e. sialic acids and heparan sulfates) in the entry of around 80 human-infective pseudotypes.

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