jezieldamasceno.bsky.social
Jeziel D. Damasceno
@jezieldamasceno.bsky.social
120 followers 91 following 6 posts
Research Associate at Richard McCulloch’s group / University of Glasgow. Leishmania biology; DNA replication; genome plasticity
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Reposted by Jeziel D. Damasceno
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Richard McCulloch @rmc9z.bsky.social · Aug 15
We’re pleased to share our new study, led by @jezieldamasceno.bsky.social, that used long-read, single molecule sequencing to reveal the full (?) picture of Leishmania DNA replication. Thanks, Mike Boemo and helpful reviewers @uofgsii.bsky.social

www.sciencedirect.com/science/arti...
Leishmania major chromosomes are replicated from a single high-efficiency locus supplemented by thousands of lower efficiency initiation events
DNA replication initiates at genome sites termed origins. Previous origin mapping approaches in the populations of the protozoan parasite Leishmania m…
www.sciencedirect.com
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Jeziel D. Damasceno @jezieldamasceno.bsky.social · Aug 6
@gabriel93dasilva.bsky.social @marquescata.bsky.social @scotomorph.bsky.social @rmc9z.bsky.social @uofglasgow.bsky.social

Big thanks to Mike Boemo
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Jeziel D. Damasceno @jezieldamasceno.bsky.social · Aug 6
Pleased 2 share our new article: single-molecule replication mapping in Leishmania using DNAscent.
We show widespread stochastic origin firing and insights into genome variability.

www.cell.com/cell-reports...
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Reposted by Jeziel D. Damasceno
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Marija Krasiļņikova @scotomorph.bsky.social · Feb 10
More recent work from the McCulloch lab! Check out Jeziel's @jezieldamasceno.bsky.social recent paper on R-loops, DNA replication and genomic stability in Leishmania major www.nature.com/articles/s41... in @naturecomms.bsky.social @rmc9z.bsky.social @marquescata.bsky.social
R-loops acted on by RNase H1 influence DNA replication timing and genome stability in Leishmania - Nature Communications
All genomes are replicated in a temporal order, though the determinants of such timing are poorly characterized. Here, the authors show that the unusual chromosome size-dependent DNA replication timin...
www.nature.com
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Reposted by Jeziel D. Damasceno
rmc9z.bsky.social
Richard McCulloch @rmc9z.bsky.social · Feb 10
Please enjoy a new paper led by @jezieldamasceno.bsky.social that reveals how R-loops and their homeostatsis through RNase H1 modulate Leishmania DNA replication timing @uofgsii.bsky.social @marquescata.bsky.social @scotomorph.bsky.social
www.nature.com/articles/s41...
R-loops acted on by RNase H1 influence DNA replication timing and genome stability in Leishmania - Nature Communications
All genomes are replicated in a temporal order, though the determinants of such timing are poorly characterized. Here, the authors show that the unusual chromosome size-dependent DNA replication timin...
www.nature.com
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jezieldamasceno.bsky.social
Jeziel D. Damasceno @jezieldamasceno.bsky.social · Feb 9
🙌🙌
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Jeziel D. Damasceno @jezieldamasceno.bsky.social · Feb 8
Excited to share our latest work on R-loops and RNAse H1, exploring their roles in genome replication timing and stability. It’s now published!

www.nature.com/articles/s41...

@rmc9z.bsky.social @marquescata.bsky.social @scotomorph.bsky.social
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Jeziel D. Damasceno @jezieldamasceno.bsky.social · Dec 10
Sneak Peak here: papers.ssrn.com/sol3/papers....

Preprint here: www.biorxiv.org/content/10.1...

@rmc9z.bsky.social @scotomorph.bsky.social @marquescata.bsky.social
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Jeziel D. Damasceno @jezieldamasceno.bsky.social · Dec 10
Fancy a sneak peek into Leishmania’s unusual DNA replication programming? At the McCulloch’s group we used D-Nascent to map origins & forks at the single-molecule level, revealing novel, abundant replication initiation events across its plastic genome. See links below👇
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