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Jim Haseloff @jimhaseloff.bsky.social · Jun 7

3D printed rotating tube mixer for incubating samples. Build instructions at: www.printables.com/model/52572-...

Reposted by Jim Haseloff

pi0cket.bsky.social @pi0cket.bsky.social · Apr 26

Building on my PCB face plate idea, how about a PCB front and back (with the main board in between) and a TPU band round the edge hugging it all in place 🤔 this is just a basic test, but I think it might just work... #Pi0cket #CL32 #ESP32

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Reposted by Jim Haseloff

Will Anderson @willaanderson.bsky.social · Dec 2

Excited to share the public release of the DIYNAFLUOR (DIY Nucleic Acid FLUORometer), a $40 USD, DIY, open-source, 3D-printed, solder-free, and portable DNA fluorometer you can build. 🦕 🧬

See our GitHub Repo for more! github.com/traulab/DIYN...

#DIYScience #STEM #DIYNAFLUOR

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Reposted by Jim Haseloff

Will Anderson @willaanderson.bsky.social · Mar 1

Dear #DIYNAFLUOR users! Please check out the "experimental" folder on our github page.
tinyurl.com/4v23f629

We have a design revision that appears to improve the LoD by ~3.5x, providing quantitation down to 0.16 ng DNA. This should cover v.short nanopore library QC.

Would love any feedback! 🧪🧬🦕❤️

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Reposted by Jim Haseloff

Louise Horsfall @lehorsfall.bsky.social · Apr 25

Unlocking the power of Engineering Biology www.gov.uk/government/n...

Unlocking the power of Engineering Biology

New report outlines the transformative potential of Engineering Biology to tackle modern-day challenges.

www.gov.uk

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Reposted by Jim Haseloff

Facundo Romani @fromani.bsky.social · Apr 14

Semi-automated workflow for high-throughput Agrobacterium-mediated plant transformation. Summary of our efforts to accelerate plant transgenics with the @earlhaminst.bsky.social Biofoundry. Now published in @theplantjournal.bsky.social. #PlantScience #Synbio onlinelibrary.wiley.com/doi/10.1111/...

Semi‐automated workflow for high‐throughput Agrobacterium‐mediated plant transformation

A simplified and optimized pipeline for Agrobacterium tumefaciens mediated transformation of plants. Useful for obtaining stable transgenic lines of the model liverwort Marchantia polymorpha, or for ...

onlinelibrary.wiley.com

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Jim Haseloff @jimhaseloff.bsky.social · Apr 6

...where a powerful toy slingshot can be used to mount the LoRa node in a tree for better line-of-sight communications with other devices.
www.instructables.com/Meshtastic-S...

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Jim Haseloff @jimhaseloff.bsky.social · Apr 6

Clever description of how to convert an inexpensive solar anchor buoy light into an extremely durable and waterproof solar powered Meshtastic node e.g. for sharing LoRa sensor data in a field setting. www.instructables.com/Meshtastic-S...

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Reposted by Jim Haseloff

Chinna Devarapu @chinnadevarapu.bsky.social · Mar 9

1/6 🧵 Excited to share Pocket Spectrometer V2! After positive feedback on the first version of pocket spectrometer. I've made significant improvements (hardware+software) while keeping what worked well with the V1 #OpenSource #Spectrometer #STEM 💡
bsky.app/profile/chin...

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Jim Haseloff @jimhaseloff.bsky.social · Mar 9

chinnadevarapu.bsky.social has updated his tiny, low cost Pocket Spectrometer design that uses an M5StickC microcontroller and AS7341 spectral sensor - simplified hardware connections and software improvements. Nice job! Details at: github.com/scientistnob...

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Reposted by Jim Haseloff

PoCaE research project @pocae.bsky.social · Dec 4

🚀✨ Exciting innovation alert! Meet the Pocket Spectrometer: a compact, open-source device 📏🔬 (< $50). Affordable, and portable, it is the open-source hardware for spectral analysis 🎨💡. Science in your pocket! 🌟👨‍🔬👩‍🔬 github.com/scientistnob...

GitHub - scientistnobee/Pocket-Spectrometer: I'm building what might be the smallest #opensource #spectrometer with an easy to use UI! I call it a pocket spectrometer. Combining #M5StickC (~$20) with ...

I'm building what might be the smallest #opensource #spectrometer with an easy to use UI! I call it a pocket spectrometer. Combining #M5StickC (~$20) with AS7341 sensor (~$17.5). My goal? A com...

github.com

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Jim Haseloff @jimhaseloff.bsky.social · Mar 4

The mini capsules can be opened and used for microscopic observation as well.
doi.org/10.1007/978-...

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Jim Haseloff @jimhaseloff.bsky.social · Mar 4

Chopping tubes and adding slits to allow the halves to fit together...
doi.org/10.1007/978-...

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Jim Haseloff @jimhaseloff.bsky.social · Mar 4

Handy little trick from Jose Ignacio Laines-Hidalgo, Angela F. Ku-Gonzalez, and Felipe A. Vazquez-Flota published in doi.org/10.1007/978-...
Making DIY small sealed capsules from Eppendorf tubes for plant histology.

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Jim Haseloff @jimhaseloff.bsky.social · Jan 2

3D print files for a collection of parts that allow micro servos to be plugged into Fischertechnik construction systems. Good for prototyping programmable mechanical devices...
www.thingiverse.com/thing:2807112

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Jim Haseloff @jimhaseloff.bsky.social · Dec 29

The described procedure for making Lenticules is relatively simple. Cells are mixed with a solution containing a high concentration of trehalose, BSA and carboxymethyl cellulose as caking agents, saline (with optional food dye), and dried on parafilm before storage over desiccant.

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Jim Haseloff @jimhaseloff.bsky.social · Dec 29

However, details for preparation of Lenticules appears in a published patent: Storage of microorganisms, cells and tissue (WO 01/05941) See at: link.lens.org/aPFhU0Bjyli

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Jim Haseloff @jimhaseloff.bsky.social · Dec 29

Lenticules were described in doi: 10.1046/j.1365-2672.1998.00611.x. by Codd et al. - but composition of the critical component for storage, the "lenticulating fluid (Codds Medium)" seems missing in this and a subsequent paper (dot: 10.1046/j.1365-2672.2001.01432.x)...

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Jim Haseloff @jimhaseloff.bsky.social · Dec 29

Have always been interested in Lenticules and Vitroids (www.sigmaaldrich.com/deepweb/asse...) - as ways of storing/distributing microbial cells as stable, dry pellets, which could be widely useful for resource sharing. Lenticules originated at Public Health England, and a protocol is available...

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Jim Haseloff @jimhaseloff.bsky.social · Dec 27

Wild collection of heirloom and genetically variant seeds available from Tomato Revolution (www.tomatorevolution.co.uk) ready for next summer.

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Reposted by Jim Haseloff

Chinna Devarapu @chinnadevarapu.bsky.social · Nov 25

1/6 🧵 I'm building what might be the smallest #opensource #spectrometer with an easy to use UI! I call it a pocket spectrometer. Combining #M5StickC (~$20) with AS7341 sensor (~$16). My goal? A complete spectrometer that is barely larger than a sample cuvette itself.

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Reposted by Jim Haseloff

Will Anderson @willaanderson.bsky.social · Dec 24

Very cool use of optomechanical elements to make a "scanning" sepctrometer.
blog.arduino.cc/2024/12/23/s...

See how this homemade spectrometer analyzes substances with an Arduino Mega | Arduino Blog

Materials, when exposed to light, will reflect or absorb certain portions of the electromagnetic spectrum that can give valuable information about their chemical or physical compositions. Traditional ...

blog.arduino.cc

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Jim Haseloff @jimhaseloff.bsky.social · Dec 24

Downloadable reference card for focus bracketing, on a site full of examples and advice about Olympus macro photography systems from Chris McGinniss
www.chrismcginnis.com/gear

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Jim Haseloff @jimhaseloff.bsky.social · Dec 24

Here's an archived free copy of CL Strong's 1960 book with collected Amateur Scientist columns from Scientific American. Inspiration for citizen science. Forward by Vannevar Bush...
archive.org/details/Proj...

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Jim Haseloff @jimhaseloff.bsky.social · Dec 24

Online portfolio of bryophyte plant images from across the globe - excellent macrophotography from Des Callaghan, captures some of the morphological diversity of this clade
bryophytes.myportfolio.com/work

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