Thanks Oli! Very impressive reconstruction in your preprint by the way! 🤩 I agree it’d be a really nice follow-up to tease apart dimer vs tetramer. On the pixel size, I did play around with different values and found 1.117 A to be about optimal.

Quality. Thou we used very stringent particle selection so there could be potential false neg.

Good point! At our resolution (Nyquist~2.2A), a 2.5/1.5 grid is generally the sweet spot for balancing efficiency and accuracy. We also tested a global search followed by local angular refinement, but didn’t observe any significant improvement.

🚀 Check out our new preprint on extending the scope of single-particle cryo-EM with 2D template matching!
Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching is now available on bioRxiv!
www.biorxiv.org/content/10.1...