Lingjun Li Research Group
@liresearch.bsky.social
Student-run account for the Lingjun Li Lab at UW-Madison. #TeamMassSpec @uwmadpharmacy @uwmadisonchem
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TEMI: tissue-expansion mass-spectrometry imaging
Nature Methods - Tissue-expansion method compatible with mass-spectrometry imaging (TEMI) enables the profiling of lipids, metabolites, peptides, proteins, and N-glycans in complex tissues with...
rdcu.be
Exciting paper in @naturemethods.bsky.social that pushes the boundaries of MSI and spatially-resolved omics🥳🥳🥳TEMI reaches single-cell spatial resolution and enables the profiling of hundreds of lipids, metabolites, peptides, and N-glycans in complex tissue. Check it out: rdcu.be/eiIWy
We're thrilled to share #AnalyticalChemistry Supplementary Cover Art for our ant proteome work 🐜🐜 Special thanks to @uwmadpharmacy.bsky.social Sally Griffith-Oh for creating this beautiful cover art and thanks to everyone for this teamwork 🥳🥳@ACSPublications #MyACSCover
Link: doi.org/10.1021/acs....
Link: doi.org/10.1021/acs....
December 31, 2025 at 9:57 PM
We're thrilled to share #AnalyticalChemistry Supplementary Cover Art for our ant proteome work 🐜🐜 Special thanks to @uwmadpharmacy.bsky.social Sally Griffith-Oh for creating this beautiful cover art and thanks to everyone for this teamwork 🥳🥳@ACSPublications #MyACSCover
Link: doi.org/10.1021/acs....
Link: doi.org/10.1021/acs....
Congratulations to Emily on successfully passing her RP and achieving the dissertator status, an important milestone of her Ph.D. journey 🥳🥳 Can't wait to see her accomplishments in the next few years ✨✨
December 23, 2025 at 4:57 PM
Congratulations to Emily on successfully passing her RP and achieving the dissertator status, an important milestone of her Ph.D. journey 🥳🥳 Can't wait to see her accomplishments in the next few years ✨✨
Huge congratulations to our 74th Ph.D., Dr. Lauren Fields @chemistlauren.bsky.social 🍾🥂✨ Thank you for all the fun and outstanding mentorship you brought to our lab 😄 Wishing you all the best in your next postdoc chapter at Prof. Maccoss Lab 🥳 We will miss you so much!
December 18, 2025 at 4:54 PM
Huge congratulations to our 74th Ph.D., Dr. Lauren Fields @chemistlauren.bsky.social 🍾🥂✨ Thank you for all the fun and outstanding mentorship you brought to our lab 😄 Wishing you all the best in your next postdoc chapter at Prof. Maccoss Lab 🥳 We will miss you so much!
Reposted by Lingjun Li Research Group
Be sure to read in our latest issue research by Eric Troncy, @liresearch.bsky.social & co. from @uwmadison.bsky.social & @umontreal.ca revealing how osteoarthritis reshapes the spinal cord peptidome in rats #proteomics
Find the full article here🔽
Find the full article here🔽
Global spinal cord peptidome profiling in response to osteoarthritis in rats
Osteoarthritis (OA) is a complex and increasingly prevalent condition, affecting an estimated 600 million people worldwide and significantly reducing quality of life. Understanding OA as both an…
pubs.rsc.org
December 17, 2025 at 4:05 PM
Be sure to read in our latest issue research by Eric Troncy, @liresearch.bsky.social & co. from @uwmadison.bsky.social & @umontreal.ca revealing how osteoarthritis reshapes the spinal cord peptidome in rats #proteomics
Find the full article here🔽
Find the full article here🔽
Reposted by Lingjun Li Research Group
It's been a year of impact for Professor Lingjun Li (@liresearch.bsky.social): She became the first-ever School of Pharmacy recipient of UW–Madison’s Kellett Mid-Career Award, and now she's being honored with a 2026 Catherine Costello Award by @us-hupo.org! https://bit.ly/44xJChr
December 16, 2025 at 5:10 PM
It's been a year of impact for Professor Lingjun Li (@liresearch.bsky.social): She became the first-ever School of Pharmacy recipient of UW–Madison’s Kellett Mid-Career Award, and now she's being honored with a 2026 Catherine Costello Award by @us-hupo.org! https://bit.ly/44xJChr
Great food, loud laughs, and a spirited White Elephant exchange at the annual Li Lab holiday party 🎄🎁
Huge congratulations to our 74th Ph.D., Dr. Lauren Fields 👩🎓🍾
So excited to welcome 10 new members to the lab family 🥳🥳
Cheers to another successful year 🥂✨
Huge congratulations to our 74th Ph.D., Dr. Lauren Fields 👩🎓🍾
So excited to welcome 10 new members to the lab family 🥳🥳
Cheers to another successful year 🥂✨
December 16, 2025 at 12:59 PM
Great food, loud laughs, and a spirited White Elephant exchange at the annual Li Lab holiday party 🎄🎁
Huge congratulations to our 74th Ph.D., Dr. Lauren Fields 👩🎓🍾
So excited to welcome 10 new members to the lab family 🥳🥳
Cheers to another successful year 🥂✨
Huge congratulations to our 74th Ph.D., Dr. Lauren Fields 👩🎓🍾
So excited to welcome 10 new members to the lab family 🥳🥳
Cheers to another successful year 🥂✨
New publication in Analytical Chemistry, in collab with Cameron Currie Lab 🥳 A dual-layered proteomic workflow that maps both whole-body and surface-secreted proteins in the leaf-cutter ant Acromyrmex echinatior. Huge congrats to Timo, Haiyan, and all co-authors 👏👏
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Proteome-Wide Analysis and Surface Protein Isolation for Secretome Characterization Reveal Insights into the Biology of the Leaf-Cutter Ant Acromyrmex echinatior
Characterizing the proteome of an organism can provide critical insights into the proteins that regulate key biological processes such as development, physiology, and environmental interactions. While...
pubs.acs.org
December 12, 2025 at 8:03 PM
New publication in Analytical Chemistry, in collab with Cameron Currie Lab 🥳 A dual-layered proteomic workflow that maps both whole-body and surface-secreted proteins in the leaf-cutter ant Acromyrmex echinatior. Huge congrats to Timo, Haiyan, and all co-authors 👏👏
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
New publication on Anal Chem 🥳🥳 Our new 3-plex TMTproC-DIA strategy delivers accurate multiplexed quantification, high precision even in complex sample backgrounds without adding spectral complexity. Congrats to Zicong, @pk-liu.bsky.social, and Haiyan 👏👏
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Multiplexed Data-Independent Acquisition-Based Proteomics Enabled by TMTpro Complementary Ions
Data-independent acquisition (DIA) has emerged as a powerful approach in quantitative proteomics, offering more comprehensive and reproducible proteome coverage than the conventional data-dependent ac...
pubs.acs.org
December 3, 2025 at 1:54 PM
New publication on Anal Chem 🥳🥳 Our new 3-plex TMTproC-DIA strategy delivers accurate multiplexed quantification, high precision even in complex sample backgrounds without adding spectral complexity. Congrats to Zicong, @pk-liu.bsky.social, and Haiyan 👏👏
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Congratulations to Graham (Jiahao) on successfully passing his prelim exam with flying colors 🥳🥳 Can't wait to see what he can accomplish with this exciting project in the coming years 🤓🤓
November 25, 2025 at 9:25 PM
Congratulations to Graham (Jiahao) on successfully passing his prelim exam with flying colors 🥳🥳 Can't wait to see what he can accomplish with this exciting project in the coming years 🤓🤓
Li Lab Friendsgiving Party ✨
A night filled with gratitude, homemade goodness, endless laughs, and the best lab family we could ask for 🍽️🧡
Sending our warmest congratulations to Dr. Hua Zhang as he embarks on his exciting new professorship journey 🥳🥳🥳
A night filled with gratitude, homemade goodness, endless laughs, and the best lab family we could ask for 🍽️🧡
Sending our warmest congratulations to Dr. Hua Zhang as he embarks on his exciting new professorship journey 🥳🥳🥳
November 23, 2025 at 7:59 PM
Li Lab Friendsgiving Party ✨
A night filled with gratitude, homemade goodness, endless laughs, and the best lab family we could ask for 🍽️🧡
Sending our warmest congratulations to Dr. Hua Zhang as he embarks on his exciting new professorship journey 🥳🥳🥳
A night filled with gratitude, homemade goodness, endless laughs, and the best lab family we could ask for 🍽️🧡
Sending our warmest congratulations to Dr. Hua Zhang as he embarks on his exciting new professorship journey 🥳🥳🥳
Our new review paper on ACA Special Issue of Imaging Mass Spec 🥳🥳 We highlight recent advances in single-cell MSI, focusing on improved spatial resolution, molecular coverage, and analytical workflows. Congrats to Hua and co-authors 👏👏
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Single-cell mass spectrometry imaging: emerging advancements and future insights
Single-cell mass spectrometry imaging (MSI) has emerged as a transformative technology for dissecting complex biological systems with high spatial pre…
www.sciencedirect.com
November 4, 2025 at 4:49 PM
Our new review paper on ACA Special Issue of Imaging Mass Spec 🥳🥳 We highlight recent advances in single-cell MSI, focusing on improved spatial resolution, molecular coverage, and analytical workflows. Congrats to Hua and co-authors 👏👏
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Costumes, candy, and chemistry 🧪🎃 Li lab turned spooky for a day 👻
November 1, 2025 at 12:19 AM
Costumes, candy, and chemistry 🧪🎃 Li lab turned spooky for a day 👻
New publication on @molecularomics.rsc.org 🥳Our work revealed how osteoarthritis reshapes the neuropeptide landscape of the spinal cord in the MIRAT model. This is a nice collaboration with Prof. Eric Troncy Lab, and congrats to Gaoyuan, Marilyn and all co-authors 👏
pubs.rsc.org/en/content/a...
pubs.rsc.org/en/content/a...
Global spinal cord peptidome profiling in response to osteoarthritis in rats
Osteoarthritis (OA) is a complex and increasingly prevalent condition, affecting an estimated 600 million people worldwide and significantly reducing quality of life. Understanding OA as both an infla...
pubs.rsc.org
October 29, 2025 at 5:55 PM
New publication on @molecularomics.rsc.org 🥳Our work revealed how osteoarthritis reshapes the neuropeptide landscape of the spinal cord in the MIRAT model. This is a nice collaboration with Prof. Eric Troncy Lab, and congrats to Gaoyuan, Marilyn and all co-authors 👏
pubs.rsc.org/en/content/a...
pubs.rsc.org/en/content/a...
Reposted by Lingjun Li Research Group
Congratulations to Prof. Lingjun Li (@liresearch.bsky.social) on earning a @uwmadison.bsky.social Kellett Mid-Career Award to support her "high risk, high reward" work in single-cell analysis & #massspectrometry, geared to understand tumors & neuron communication! pharmacy.wisc.edu/2025/09/10/p...
October 23, 2025 at 4:07 PM
Congratulations to Prof. Lingjun Li (@liresearch.bsky.social) on earning a @uwmadison.bsky.social Kellett Mid-Career Award to support her "high risk, high reward" work in single-cell analysis & #massspectrometry, geared to understand tumors & neuron communication! pharmacy.wisc.edu/2025/09/10/p...
Fall tradition continues 🍂🍁 The Li Lab returned to Eugster’s Farm for another day of apple picking, pumpkin hunting, and apple cider donut 🍎🎃🍩 Grateful for great weather and great company with our rotation students 🥳🥳
October 19, 2025 at 2:44 PM
Fall tradition continues 🍂🍁 The Li Lab returned to Eugster’s Farm for another day of apple picking, pumpkin hunting, and apple cider donut 🍎🎃🍩 Grateful for great weather and great company with our rotation students 🥳🥳
Reposted by Lingjun Li Research Group
🚨 A new study from Professor Lingjun Li @liresearch.bsky.social and collaborators in the Molecular Psychiatry journal finds that changes in an enzyme that helps #neurons function properly can alter synaptic function and drive #autism-like behaviors in a model.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Increased expression of ATase1/NAT8B or ATase2/NAT8 in the mouse results in an autistic-like phenotype with altered dendritic branching and spine formation - Molecular Psychiatry
Molecular Psychiatry - Increased expression of ATase1/NAT8B or ATase2/NAT8 in the mouse results in an autistic-like phenotype with altered dendritic branching and spine formation
www.nature.com
October 10, 2025 at 4:18 PM
🚨 A new study from Professor Lingjun Li @liresearch.bsky.social and collaborators in the Molecular Psychiatry journal finds that changes in an enzyme that helps #neurons function properly can alter synaptic function and drive #autism-like behaviors in a model.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Jeopardy night, round 3 🎉 Another year of laughter, surprises, and impressive fun facts from our lab crew 😆 Always a highlight seeing everyone’s hidden talents (and competitive side) ✨ Shoutout to Lauren, Jacey, and Kelly for organizing all the fun 👏
October 8, 2025 at 12:57 PM
Jeopardy night, round 3 🎉 Another year of laughter, surprises, and impressive fun facts from our lab crew 😆 Always a highlight seeing everyone’s hidden talents (and competitive side) ✨ Shoutout to Lauren, Jacey, and Kelly for organizing all the fun 👏
Excited to share our latest work on Analytical Chemistry, introducing a novel 13-plex DeAla isobaric tag for improved peptide fragmentation, enhanced protein identification, and competitive pricing 🥳🥳🥳 Congrats to @pk-liu.bsky.social and co-authors ✨✨
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
13-Plex DeAla Isobaric Reagents for High-Throughput Proteome Quantification
Isobaric labeling techniques are widely used in mass spectrometry-based quantitative proteomics to enable the simultaneous analysis of multiple samples. However, commercial isobaric tags are expensive...
pubs.acs.org
October 7, 2025 at 3:22 PM
Excited to share our latest work on Analytical Chemistry, introducing a novel 13-plex DeAla isobaric tag for improved peptide fragmentation, enhanced protein identification, and competitive pricing 🥳🥳🥳 Congrats to @pk-liu.bsky.social and co-authors ✨✨
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
New publication on Analytica Chimica Acta 🥳🥳 We presented 3D spatial proteomic and lipidomic analysis of AD and WT mouse brains via 10-plex immunohistochemistry (IHC)-based photocleavable mass tag (PC-MT) labeling. Congrats Kelly and co-authors 👏👏
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Deciphering the Three-Dimensional Biomolecular Distribution in the Alzheimer’s Disease Brain: A Multiomic Approach Integrating Immunohistochemistry with MALDI MS Imaging
Alzheimer’s disease (AD) is characterized by complex and distinct spatial and molecular changes in the brain that contribute to its onset and progress…
www.sciencedirect.com
October 3, 2025 at 7:07 PM
New publication on Analytica Chimica Acta 🥳🥳 We presented 3D spatial proteomic and lipidomic analysis of AD and WT mouse brains via 10-plex immunohistochemistry (IHC)-based photocleavable mass tag (PC-MT) labeling. Congrats Kelly and co-authors 👏👏
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Celebrating the successful 3rd International Single Cell Mass Spec conference in Chicago last weekend🥳 Congrats to Kelly for winning Best Poster Award and Hua for Young Investigator Award in the category of best advancement award recognizing his scientific accomplishments👏
September 30, 2025 at 1:13 PM
Celebrating the successful 3rd International Single Cell Mass Spec conference in Chicago last weekend🥳 Congrats to Kelly for winning Best Poster Award and Hua for Young Investigator Award in the category of best advancement award recognizing his scientific accomplishments👏
New research published on Nature @molpsychiatry 🥳🥳 Neuron-specific overexpression of ATase1/ATase2 in mice leads to autistic-like behaviors via ER acetylation pathway disruptions. Congrats Haiyan and a nice collab with Luigi Puglielli Lab
Link 👇👇
www.nature.com/articles/s41...
Link 👇👇
www.nature.com/articles/s41...
Increased expression of ATase1/NAT8B or ATase2/NAT8 in the mouse results in an autistic-like phenotype with altered dendritic branching and spine formation - Molecular Psychiatry
Molecular Psychiatry - Increased expression of ATase1/NAT8B or ATase2/NAT8 in the mouse results in an autistic-like phenotype with altered dendritic branching and spine formation
www.nature.com
September 29, 2025 at 4:31 PM
New research published on Nature @molpsychiatry 🥳🥳 Neuron-specific overexpression of ATase1/ATase2 in mice leads to autistic-like behaviors via ER acetylation pathway disruptions. Congrats Haiyan and a nice collab with Luigi Puglielli Lab
Link 👇👇
www.nature.com/articles/s41...
Link 👇👇
www.nature.com/articles/s41...
Congratulations to Zach, our @uwmadpharmacy.bsky.social student, on successfully passing the prelim exam and officially becoming a Ph.D. Candidate 🥳🥳🥳 We can't wait to hear about your exciting research in the near future 👏👏👏
September 26, 2025 at 11:29 PM
Congratulations to Zach, our @uwmadpharmacy.bsky.social student, on successfully passing the prelim exam and officially becoming a Ph.D. Candidate 🥳🥳🥳 We can't wait to hear about your exciting research in the near future 👏👏👏
Big congrats to David 🥳 🥳 our highly motivated high school summer intern, on successfully presenting his research at the 29th Annual High School Research Poster Session 👏👏
September 25, 2025 at 2:45 PM
Big congrats to David 🥳 🥳 our highly motivated high school summer intern, on successfully presenting his research at the 29th Annual High School Research Poster Session 👏👏
Check out this latest paper in the special issue #Biomolecules Molecular Effects of Environmental Pollutants on Health of Human,Animals and Plants 🥳 🥳We use iDiLeu to quantify metabolite changes upon copper exposure! Congrats to Olga 😃😃
Link below 👇👇
www.mdpi.com/2218-273X/15...
Link below 👇👇
www.mdpi.com/2218-273X/15...
www.mdpi.com
September 4, 2025 at 2:01 PM
Check out this latest paper in the special issue #Biomolecules Molecular Effects of Environmental Pollutants on Health of Human,Animals and Plants 🥳 🥳We use iDiLeu to quantify metabolite changes upon copper exposure! Congrats to Olga 😃😃
Link below 👇👇
www.mdpi.com/2218-273X/15...
Link below 👇👇
www.mdpi.com/2218-273X/15...
We're thrilled to celebrate Dr. Zhang, the 72nd Ph.D. from the Li Lab 🥳🥳🥳 Wishing you success and happiness in all your future endeavors 😃
September 3, 2025 at 11:35 PM
We're thrilled to celebrate Dr. Zhang, the 72nd Ph.D. from the Li Lab 🥳🥳🥳 Wishing you success and happiness in all your future endeavors 😃