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Light-Sheet Fluorescence Microscopy (LSFM) course

@lsfm-na.bsky.social

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This is the US version of the long running course Pavel and Emmanuel have been running. We are just this side of the pond. Being held at MDIBL in Bar Harbor, Maine, June 2-6, 2025.

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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Apr 3

12 systems will be there, Bruker x3!, 3i, Leica, ASI, MBF, LiT, LifeCanvas x2!, and Miltenyi! Amazing cutting-edge technology, accessible instructors, and Bar Harbor! Please re-bluesky (what do you call it here:))!

MDI Biological Laboratory @mdibiolab.bsky.social · Apr 3

Have you been thinking of enrolling the MDI Bio Lab's #LightSheet Fluorescence Microscopy course?

Registration has been lowered to $1,000 — down from $3,500!
This is a great opportunity for researchers/students to enhance their knowledge & skills in cutting-edge #microscopy techniques. 🧪 🤝 🍎 👩‍🔬

Light Sheet Fluorescence Microscopy 2025

mdibl.org

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Brandy A Freitas @brandy-syglass.bsky.social · Jun 9

This was a great sample to check out -- who knew 100M year old amber would be so close to Ethyl Cinnamate in RI? Huge shout out to @brukercorporation.bsky.social for the fleet of lightsheets at @lsfm-na.bsky.social last week!

New England Society for Microscopy @nesmicroscopy.bsky.social · Jun 9

For #MicroscopyMonday, a 100M year-old 🦟 in amber! Lots of familiar #NESM faces up in Acadia for the #LSFM25 at the MDI @mdibiolab.bsky.social !

🔬: Bruker @brukercorporation.bsky.social, LCS SPIM
🟠: Courtesy the Sims Collection
🥼&🎥: Jan Frankowski @frankowskijc.bsky.social

@lsfm-na.bsky.social

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New England Society for Microscopy @nesmicroscopy.bsky.social · Jun 9

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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 7

And with that, #lsfm25 comes to a close! We want to thank everyone who helped make this possible - the faculty, course directors, TAs, vendors, and sponsors. But most importantly the many wonderful people at MDIBL. See you all next year!

Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 6

Preparation for the final event of the course: a boat tour of Acadia!

Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 6

Now that the students have had several days to learn sample preparation and image acquisition, we are wrapping up day five with a crash course called "Now what do you do with your data?" Led by @dpshepherd.bsky.social and Carsten Wolff.

#lsfm25 #microscopy #science #education

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Doug Shepherd @dpshepherd.bsky.social · Jun 4

Spending some time at #lsfm25 cleaning up code. It now has distributed GPU gradient-consensus RL decon (@jamesdmanton.bsky.social @andrewgyork.bsky.social) our fast orthogonal deskew, and stitching/fusion to ome-ngff v0.5. Example AO-OPM data on uncleared mouse brain slice github.com/QI2lab/opm-p...

Adaptive optics oblique plane microscopy of 100 um thick mouse brain slice with immunofluorescence.

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Brandy A Freitas @brandy-syglass.bsky.social · Jun 5

Up in Acadia at the @mdibiolab.bsky.social for #LSFM25! Here's a cleared whole mouse head on the SmartSPIM, 3.6X objective for #3DThursday.

🔎 @jtakatoh.bsky.social, now at @stonybrooku.bsky.social
🔬 LifeCanvas Technologies, lifecanvastech.com/products/sma...
🥽 syGlass.io

@lsfm-na.bsky.social

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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 5

We are so excited to start day four with a wonderful talk from Kara Cerveny!

#lsfm25 #science #microscopy #education
@mdibiolab.bsky.social
@kara-cerveny.bsky.social

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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 4

It's day three and we are starting our morning off with an excellent intro to clearing by Doug Richardson.

#lsfm25 #microscopy #science #education
@mdibiolab.bsky.social

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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 3

And it begins....

Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 3

The calm before the storm indeed! In just a half an hour, this board will be bombarded by 25 students rushing to grab a spot on their instrument of choice. 11 systems. Only 9 labs. May the odds be ever in their favor...

#lsfm25 #microscopy #science #education
@mdibiolab.bsky.social

Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 1

Calm before the storm…

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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 3

We are back from lunch with a talk from Raghav Chhetri telling us all about using light sheet microscopy for neuroscience imaging!

#lsfm25 #microscopy #science #education
@mdibiolab.bsky.social
@rchhmiscope.bsky.social

Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 3

It's day two! We're starting off a day filled with science with a talk from Anna-Karin Gustavsson from Rice University.

#lsfm25 #microscopy #science #education

@mdibiolab.bsky.social
@riceuniversity.bsky.social
@gustavssonlab.bsky.social

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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 3

Morning in Bar Harbor doesn’t suck.

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Emmanuel G. Reynaud @ereynaud.bsky.social · Jun 3

Step 8: In @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Well day one done worth a sunset!

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Emmanuel G. Reynaud @ereynaud.bsky.social · Jun 2

Step @lsfm-na.bsky.social 2025 at @mdibiolab.bsky.social‬ organized by @glaevsky.bsky.social. Went to collect some samples... Here is a pink tunic ate!

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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 2

Reto Fiolka and OPM for the Keynote!

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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 2

labs.utsouthwestern.edu/fiolka-lab

Fiolka Lab | UT Southwestern, Dallas, Texas

The long-term goal of Fiolka Lab's research is to develop and implement imaging technologies that provide unprecedented insight into cancer biology.

labs.utsouthwestern.edu

Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 2

With the end of our first round of vendor talks behind us, we are excited to welcome Reto Fiolka as our keynote speaker this year.

#lsfm25 #microscopy #science #education
@mdibiolab.bsky.social

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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 2

Holly Gibbs discussing the logistics of image processing and analysis

Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 2

While we are on our lunch break, the students are enjoying some beautiful weather here at @mdibiolab.bsky.social.

#lsfm25 #microscopy #science #education

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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 2

We now have Vera Morales from Nikon telling us all about objectives: the why, when, and how!

#lsfm25 #microscopy #science #education

Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 2

Claudette St Croix from CBI kicks off LSFM 2025 at @mdibiolab.bsky.social

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Light-Sheet Fluorescence Microscopy (LSFM) course @lsfm-na.bsky.social · Jun 2

We are thrilled to have you, Melissa!

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Melissa Cooper, PhD @melcooperphd.bsky.social · Jun 2

@lsfm-na.bsky.social Is this heaven, or am I just currently surrounded by ELEVEN DIFFERENT LIGHT SHEET MICROSCOPES? So thrilled to be out here in Acadia chatting about microscopy!

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