This work is the result of fantastic teamwork! #Integrative structural cell biology
@mrclmb.bsky.social, @biorxivpreprint.bsky.social

Our findings reveal a unique splice variant and a trapped substrate intermediate never observed before, offering new insights into proteolytic catalysis. We also show proteasomes cluster from the post-meiotic spermatid stage, with striking human-mouse differences in proteasome organisation!

We discovered giant membrane-less islands of proteasomes in human sperm nuclei, containing 20S and 20S-PA200 populations! We solved their in situ structure at sub-nanometer resolution (up to 6.8 Å) and in vitro at near-atomic resolution (1.8 Å)!

Thank you Kanishka for this piece! @tom-hale.bsky.social ky.social, @a-dsantos.bsky.social y.social , @mrclmb.bsky.social

Many congratulations!! Really great work!

Also check out the work of @tzviyazbm.bsky.social on the same topic!

It was a lot of fun and a tremendous synergy of intents dear @rezamehdipour.bsky.social! Thank you

We discovered that the SUN5-Nesprin3 LINC complex forms a hexagonal lattice at the sperm HTCA, which radically reshapes the nuclear membrane locally!
Our findings also provide the structural basis for understanding the severe ASS (Acephalic Sperm Syndrome) male infertility phenotype.

This work is the result of a fantastic collaboration [email protected]!
@warred.bsky.social, @a-dsantos.bsky.social , @alisterburt.bsky.social , @tom-hale.bsky.social, @mrclmb.bsky.social,