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matteogravino.bsky.social
Matteo Gravino
@matteogravino.bsky.social
33 followers 37 following 9 posts
Scientist at the John Innes Centre. Interested in plant-aphid interactions.
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matteogravino.bsky.social
Matteo Gravino @matteogravino.bsky.social · Aug 1
🎉Thrilled to share our latest work, now published in @newphyt.bsky.social!

🧑‍🔬 @matteogravino.bsky.social, @samtmugford.bsky.social, @saskiahogenhout.bsky.social et al., @johninnescentre.bsky.social

#️⃣ #PlantScience #PlantImmunity

📄 Read the full paper👇
nph.onlinelibrary.wiley.com/share/8QSRJ9...
Model: Activation and suppression of defences in plant–aphid interactions. Upper left panel: aphid stylet penetration of the cell wall releases oligogalacturonides (OGs), which induce PAMP/DAMP-triggered immunity (PTI/DTI) in a process dependent on BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1 (BAK1), ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), and CPK5/6/11 (data herein). The stabilisation of pattern recognition receptors (PRRs) and coreceptors at the plasma membrane may rely on the deubiquitination (DUB) activity of ASSOCIATED MOLECULE WITH THE SH3 DOMAIN OF STAM (AMSHs; Gravino et al., 2024). Upper right panel: the Mp10 effector, introduced by aphids into the cell cytoplasm (Mugford et al., 2016), suppresses OG-induced reactive oxygen species (ROS) (data herein) and flg22-induced PTI (Bos et al., 2010). Mp10 targeting of plant AMSHs is implicated in these processes (Gravino et al., 2024). EDS1 is essential for Mp10-mediated ROS suppression and PRR destabilisation (data herein), acting through an unidentified mechanism (denoted by the double-sided arrow with an asterisk). Lower left panel: effector-triggered immunity (ETI) is activated through EDS1, either directly or indirectly, upon recognition of Mp10 and/or its activities by a TNL (Gravino et al., 2024; Rao et al., 2024). Salicylic acid glucosyltransferase 1 (SGT1) is also required for TNL/ETI activation (Bos et al., 2010). Lower right panel: aphids secrete additional effectors, such as cathepsin B proteins (e.g. CathB6), which target EDS1 to suppress ETI (Liu et al., 2025). Solid arrows, increased activation; solid blunt-ended arrows, increased suppression; Dashed arrows, reduced activation; Dashed -blunt-ended arrows, reduced suppression.
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Reposted by Matteo Gravino
samtmugford.bsky.social
Sam Mugford @samtmugford.bsky.social · Jul 31
Now published in @newphyt.bsky.social , congrats @matteogravino.bsky.social @saskiahogenhout.bsky.social et al @johninnescentre.bsky.social nph.onlinelibrary.wiley.com/doi/10.1111/...
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matteogravino.bsky.social
Matteo Gravino @matteogravino.bsky.social · Aug 1
8/
🌟Congrats to the amazing team:
@matteogravino.bsky.social
@samtmugford.bsky.social
Daniela Pontiggia
Joshua Joyce
Claire Drurey
David Prince
Felice Cervone
Giulia De Lorenzo
@saskiahogenhout.bsky.social

🙏Thanks to editor Carlos Ballaré & referees for their valuable feedback!
matteogravino.bsky.social
Matteo Gravino @matteogravino.bsky.social · Aug 1
7/
🧬Our model also includes recent findings:

· Mp10’s immune suppression involves interaction with AMSH deubiquitinases
👉 Matteo Gravino and @samtmugford.bsky.social work: bsky.app/profile/samt...

· Other aphid effectors actively target EDS1
👉 @qunliu07.bsky.social's work: bsky.app/profile/qunl...
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matteogravino.bsky.social
Matteo Gravino @matteogravino.bsky.social · Aug 1
6/
🔑 Conclusion: EDS1 mediates Mp10's dual function—suppressing DTI/PTI and triggering ETI.

🧩 Mp10 operates at the crossroads of PTI/DTI and ETI, manipulating plant immunity to the aphid’s advantage.
Model: Activation and suppression of defences in plant–aphid interactions. Upper left panel: aphid stylet penetration of the cell wall releases oligogalacturonides (OGs), which induce PAMP/DAMP-triggered immunity (PTI/DTI) in a process dependent on BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1 (BAK1), ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), and CPK5/6/11 (data herein). The stabilisation of pattern recognition receptors (PRRs) and coreceptors at the plasma membrane may rely on the deubiquitination (DUB) activity of ASSOCIATED MOLECULE WITH THE SH3 DOMAIN OF STAM (AMSHs; Gravino et al., 2024). Upper right panel: the Mp10 effector, introduced by aphids into the cell cytoplasm (Mugford et al., 2016), suppresses OG-induced reactive oxygen species (ROS) (data herein) and flg22-induced PTI (Bos et al., 2010). Mp10 targeting of plant AMSHs is implicated in these processes (Gravino et al., 2024). EDS1 is essential for Mp10-mediated ROS suppression and PRR destabilisation (data herein), acting through an unidentified mechanism (denoted by the double-sided arrow with an asterisk). Lower left panel: effector-triggered immunity (ETI) is activated through EDS1, either directly or indirectly, upon recognition of Mp10 and/or its activities by a TNL (Gravino et al., 2024; Rao et al., 2024). Salicylic acid glucosyltransferase 1 (SGT1) is also required for TNL/ETI activation (Bos et al., 2010). Lower right panel: aphids secrete additional effectors, such as cathepsin B proteins (e.g. CathB6), which target EDS1 to suppress ETI (Liu et al., 2025). Solid arrows, increased activation; solid blunt-ended arrows, increased suppression; Dashed arrows, reduced activation; Dashed -blunt-ended arrows, reduced suppression.
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matteogravino.bsky.social
Matteo Gravino @matteogravino.bsky.social · Aug 1
5/
We found Mp10's effect is EDS1-dependent.
Without EDS1:
· No ETI
· PRRs stabilize
· DTI/PTI aren't suppressed
⏩ Aphids colonise more easily
1
matteogravino.bsky.social
Matteo Gravino @matteogravino.bsky.social · Aug 1
4/
However, Mp10 also triggers ETI-like responses, including chlorosis—ultimately reducing aphid success. Why?
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matteogravino.bsky.social
Matteo Gravino @matteogravino.bsky.social · Aug 1
3/
But aphids fight back. How?

🛡️ They suppress OG production upon wounding

🧬 And deploy Mp10, an aphid-secreted saliva effector protein that:
· Reduces OG/flg22 sensitivity
· Destabilises pattern recognition receptors (PRRs)
1
matteogravino.bsky.social
Matteo Gravino @matteogravino.bsky.social · Aug 1
2/
💥 DTI—triggered by the plant cell-wall-derived fragments oligogalacturonides (OGs)—strongly limits aphid colonisation. This defence requires:
· BAK1/BKK1
· CPK5/6/11
· GRP3
· EDS1
1
matteogravino.bsky.social
Matteo Gravino @matteogravino.bsky.social · Aug 1
🧵1/
We explored how the polyphagous peach–potato aphid (Myzus persicae) colonises Arabidopsis thaliana, and uncovered a key role for DAMP-triggered immunity (DTI).
Peach–potato aphid (Myzus persicae)
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matteogravino.bsky.social
Matteo Gravino @matteogravino.bsky.social · Aug 1
🎉Thrilled to share our latest work, now published in @newphyt.bsky.social!

🧑‍🔬 @matteogravino.bsky.social, @samtmugford.bsky.social, @saskiahogenhout.bsky.social et al., @johninnescentre.bsky.social

#️⃣ #PlantScience #PlantImmunity

📄 Read the full paper👇
nph.onlinelibrary.wiley.com/share/8QSRJ9...
Model: Activation and suppression of defences in plant–aphid interactions. Upper left panel: aphid stylet penetration of the cell wall releases oligogalacturonides (OGs), which induce PAMP/DAMP-triggered immunity (PTI/DTI) in a process dependent on BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1 (BAK1), ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), and CPK5/6/11 (data herein). The stabilisation of pattern recognition receptors (PRRs) and coreceptors at the plasma membrane may rely on the deubiquitination (DUB) activity of ASSOCIATED MOLECULE WITH THE SH3 DOMAIN OF STAM (AMSHs; Gravino et al., 2024). Upper right panel: the Mp10 effector, introduced by aphids into the cell cytoplasm (Mugford et al., 2016), suppresses OG-induced reactive oxygen species (ROS) (data herein) and flg22-induced PTI (Bos et al., 2010). Mp10 targeting of plant AMSHs is implicated in these processes (Gravino et al., 2024). EDS1 is essential for Mp10-mediated ROS suppression and PRR destabilisation (data herein), acting through an unidentified mechanism (denoted by the double-sided arrow with an asterisk). Lower left panel: effector-triggered immunity (ETI) is activated through EDS1, either directly or indirectly, upon recognition of Mp10 and/or its activities by a TNL (Gravino et al., 2024; Rao et al., 2024). Salicylic acid glucosyltransferase 1 (SGT1) is also required for TNL/ETI activation (Bos et al., 2010). Lower right panel: aphids secrete additional effectors, such as cathepsin B proteins (e.g. CathB6), which target EDS1 to suppress ETI (Liu et al., 2025). Solid arrows, increased activation; solid blunt-ended arrows, increased suppression; Dashed arrows, reduced activation; Dashed -blunt-ended arrows, reduced suppression.
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Reposted by Matteo Gravino
samtmugford.bsky.social
Sam Mugford @samtmugford.bsky.social · Jul 16
Aphid Saliva fans, happy news, this amazing story is now out in Science Advances www.science.org/doi/10.1126/... congrats @qunliu07.bsky.social @saskiahogenhout.bsky.social et al @johninnescentre.bsky.social 24h after a triumphant presentation at #ismpmi2025
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Reposted by Matteo Gravino
baptistebio.bsky.social
Baptiste Castel @baptistebio.bsky.social · Mar 20
🔗 Preprint alert: www.biorxiv.org/content/10.1...

🧵 New insights into plant immunity: using ferns to understand evolution! 🌱✨🌿

Excited to share my first (but not last) published research at @lrsv-toulouse.bsky.social. A THREAD … [1/9]
Exploring fern pathosystems and immune receptors to bridge gaps in plant immunity
Land plants include angiosperms, bryophytes, lycophytes and ferns, each of which may deploy distinct strategies to resist pathogens. Here, we investigate fern-pathogen interactions by characterizing n...
www.biorxiv.org
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Reposted by Matteo Gravino
somssich.bsky.social
Marc Somssich @somssich.bsky.social · Mar 3
📜 Aphid effector Mp10 balances immune suppression and defence activation through EDS1-dependent modulation of plant DAMP responses

🧑‍🔬 @matteogravino.bsky.social, @saskiahogenhout.bsky.social, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantImmunity
www.biorxiv.org
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Reposted by Matteo Gravino
samtmugford.bsky.social
Sam Mugford @samtmugford.bsky.social · Mar 1
We are excited to share our latest preprint from the Hogenhout lab (@matteogravino.bsky.social @saskiahogenhout.bsky.social
, @johninnescentre.bsky.social
) on plant-aphid interactions, showing that the aphid effector Mp10 balances suppression of DAMP responses and activation of ETI via EDS1.
biorxiv-plants.bsky.social
bioRxiv Plant Bio @biorxiv-plants.bsky.social · Feb 28
Aphid effector Mp10 balances immune suppression and defence activation through EDS1-dependent modulation of plant DAMP responses https://www.biorxiv.org/content/10.1101/2025.02.26.640334v1
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Reposted by Matteo Gravino
samtmugford.bsky.social
Sam Mugford @samtmugford.bsky.social · Nov 18
We’re delighted to share our latest preprint from the Hogenhout lab (@saskiahogenhout.bsky.social) on the molecular mechanisms on plant-aphid interactions. Here we show how the aphid Mp10 effector protein acts as a local anaesthetic to suppress the plant perception of aphid attack
biorxiv-plants.bsky.social
bioRxiv Plant Bio @biorxiv-plants.bsky.social · Nov 17
The conserved aphid saliva chemosensory protein effector Mp10 targets plant AMSH deubiquitinases at cellular membranes to suppress pattern-triggered immunity https://www.biorxiv.org/content/10.1101/2024.11.15.622802v1
The conserved aphid saliva chemosensory protein effector Mp10 targets plant AMSH deubiquitinases at cellular membranes to suppress pattern-triggered immunity https://www.biorxiv.org/content/10.1101/2024.11.15.622802v1
Chemosensory proteins (CSPs) are a conserved family present in insects and other arthropods, recogni
www.biorxiv.org
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