Lightnews — Scholar-powered news

Michalis Averof @michalis-averof.bsky.social · 5d

Cool collaborations on light sheet microscopy @huiskenlab.bsky.social, cellular barcoding @mgrillo.bsky.social, AI-based cell tracking @jytinevez.bsky.social, and citizen science through video game for cell tracking @rebelpixel.fr

see www.averof-lab.org/pages/tracman

3/3

1 1 6

Michalis Averof @michalis-averof.bsky.social · 5d

Postdoc will:
* Βe trained in live imaging & transgenic approach
* Generate fluorescent markers for sensory organ cell types (neurons, setae, support cells & glia)
* Develop recombinase-based cell barcoding
* Use barcoding, live imaging & cell tracking to identify regenerative progenitors

2/3

1 2 4

Michalis Averof @michalis-averof.bsky.social · 5d

Here it is! Postdoctoral position to identify the progenitors sensory organs in the regenerating legs of Parhyale

apply here: www.averof-lab.org/pages/tracman

1/3

Michalis Averof @michalis-averof.bsky.social · Jul 13

We've just been awarded a grant to study the cellular basis of regeneration – to track the progenitors of sensory organs in the context of leg regeneration, in our favourite crustacean tinyurl.com/parhyale, based on live imaging and cell tracking. The project involves some cool collaborations... 1/3

1 35 50

Michalis Averof @michalis-averof.bsky.social · 5d

localized to the plasma membrane? ;)

1

Michalis Averof @michalis-averof.bsky.social · 14d

X-ray imaging of crustacean legs at the DESY synchrotron, Hamburg. Cross-section of regenerating Parhyale leg (~1/10 of a mm in width). X-rays give accurate 3D view with almost cellular resolution. With Angelika Svetlove, Kevin Grüner & Mathilde Paris @zoocell.biologists.social.ap.brid.gy

3 20

Michalis Averof @michalis-averof.bsky.social · Sep 2

Interested in a sabbatical in Lyon? The Collegium de Lyon provides accommodation and attractive interdisciplinary environment for 1 or 2 semesters. Open to all disciplines. You can be affiliated with any local department/lab. Apply now for 2026-27. collegium.universite-lyon.fr/2026-2027-un...

3 37 57

Michalis Averof @michalis-averof.bsky.social · Aug 31

We describe the nucleus as the administrator of the cell. It shares two attributes with administrators: it perpetuates its kind & defies so successfully all efforts to learn what it is doing that only by trying to get along without it can we satisfy ourselves that it is working at all (D.Mazia 1962)

1 1 9

Michalis Averof @michalis-averof.bsky.social · Aug 9

very interesting, thank you!

1

Michalis Averof @michalis-averof.bsky.social · Aug 8

Thanks and congratulations to everyone involved in this work. Grateful to EU @erc.europa.eu and France @agencerecherche.bsky.social for supporting this project. 6/6

4

Michalis Averof @michalis-averof.bsky.social · Aug 8

Our next objective: develop fluorescent markers to identify & track the progenitors of every cell type in the leg, starting from the cells that make up the leg's sensory organs. Watch this space for a postdoctoral opening in fall. bsky.app/profile/mich... 5/6

Michalis Averof @michalis-averof.bsky.social · Jul 13

We've just been awarded a grant to study the cellular basis of regeneration – to track the progenitors of sensory organs in the context of leg regeneration, in our favourite crustacean tinyurl.com/parhyale, based on live imaging and cell tracking. The project involves some cool collaborations... 1/3

3 6

Michalis Averof @michalis-averof.bsky.social · Aug 8

In the latest paper, @cagricevrim.bsky.social, @berylbiologist.bsky.social‬ & Ko succeeded in imaging the entire course of leg regeneration and @mlrusciano.bsky.social‬ & @almazan.bsky.social developed HCR to identify specific cell types after imaging. elifesciences.org/articles/107... 4/6

Long-term live imaging, cell identification and cell tracking in regenerating crustacean legs

Live imaging allows tracking cellular behaviours and cell fates during the entire course of leg regeneration, spanning approximately one week, in a crustacean model.

elifesciences.org

1 2 5

Michalis Averof @michalis-averof.bsky.social · Aug 8

Next, Ko Sugawara developed the Elephant software to use AI for tracking cells. Elephant performs deep learning in an incremental/interactive manner, improving the software as the user checks and corrects the predicted tracks. elifesciences.org/articles/69380 3/6

Tracking cell lineages in 3D by incremental deep learning

Incremental deep learning enables accurate tracking of cell lineages in 3D with a modest investment in time and effort.

elifesciences.org

1 3

Michalis Averof @michalis-averof.bsky.social · Aug 8

This work started nearly a decade ago, when Frederike Alwes developed a method for immobilising the legs of transgenic Parhyale under a confocal microscope. elifesciences.org/articles/19766 2/6

Live imaging reveals the progenitors and cell dynamics of limb regeneration

Long-term live imaging reveals the dynamic cell behaviors and progenitor cells that underpin the regeneration of adult limbs in the crustacean Parhyale hawaiensis.

elifesciences.org

1

Michalis Averof @michalis-averof.bsky.social · Aug 8

Latest paper elifesciences.org/articles/107... closes an important cycle in our efforts to study regeneration: week-long recordings allow us to observe the behaviour of cells during the entire course of regeneration in a crustacean leg – bright objects in movie are fluorescent nuclei of cells. 1/6

2 50 140

Michalis Averof @michalis-averof.bsky.social · Jul 13

We'll be looking for a postdoc to bring these approaches together, funded for 3+ years. If you are interested please get in touch; if you know someone who might be interested please share this message. 3/3

2 6 20

Michalis Averof @michalis-averof.bsky.social · Jul 13

for light sheet microscopy w. Flamingo team @metteh-thorsager.bsky.social, genetic barcoding w. @mgrillo.bsky.social, and a video game for cell tracking w. @jytinevez.bsky.social. We'll apply these tools to discover the progenitors for sense organ regeneration in Parhyale. 2/3

1 19

Michalis Averof @michalis-averof.bsky.social · Jul 13

We've just been awarded a grant to study the cellular basis of regeneration – to track the progenitors of sensory organs in the context of leg regeneration, in our favourite crustacean tinyurl.com/parhyale, based on live imaging and cell tracking. The project involves some cool collaborations... 1/3

7 38 190

Michalis Averof @michalis-averof.bsky.social · Jun 28

including "panoramic" key regulators, a new term in the hype toolkit ;)

1 1

Reposted by Michalis Averof

Arvid Ågren @arvidagren.bsky.social · Jun 27

John Maynard Smith on natural history.

(London Review of Books, April 1982)

7 22

Michalis Averof @michalis-averof.bsky.social · Jun 25

diverse body shapes of Trichoplax, from small pancake to irregular spaghetti

1 4

Michalis Averof @michalis-averof.bsky.social · Jun 23

In the brain of intertidal crustaceans (Eurydice and Parhyale), circadian clock genes are expressed with a daily rhythm (24h) in some cells and a tidal rhythm (12.4h) in other cells. Two different clocks working in the same brain, using the same components.

Expression of clock genes tracks daily and tidal time in brains of intertidal crustaceans Eurydice pulchra and Parhyale hawaiensis

Intertidal organisms, such as the crustaceans Eurydice pulchra and Parhyale hawaiensis, express daily and tidal rhythms of physiology and behavior to …

www.sciencedirect.com

7 30

Michalis Averof @michalis-averof.bsky.social · Jun 22

I think you can also get eduroam in swedish airports

1 3

Michalis Averof @michalis-averof.bsky.social · May 10

Trichoplax adhaerens is one of the simplest and most enigmatic animals on earth. Its body shows incredible fluidity, changing shape within minutes. In a recent visit by Andrea Pasini we had the chance to host and observe these amazing animals live.
[movie accelerated 3x; animal 0.5 to 1 mm in size]

12 28 180

Reposted by Michalis Averof

Cnidarian developmental mechanisms group @clytia-vlfr.bsky.social · May 5

Intriguing findings from our Sendai collaborators Ruka Kitsui and Ryusaku Deguchi. While C. hemispherica spawns at dawn, Clytia IZ-D species spawns at dusk. This is not triggered by dark but, unexpectedly, by light-mediated extension of an autonomously-running 20h cycle. doi.org/10.1101/2025...

Cartoon of autonomous and light-modulated spwning cycles in Clytia IZ-D

7 14

Michalis Averof @michalis-averof.bsky.social · Mar 7

"To show our solidarity with our colleagues in the United States who live in fear, and to raise awareness of the abuses that are taking place, fortunately on a much smaller scale but still worrying, in Europe and France."

1 2 14