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Misha Kudryashev @mishakudryashev.bsky.social · 7d

Group Leader in Biomedical Engineering at the @mdc-berlin.bsky.social in Berlin.

It's a broad call for whoever is doing exciting Bioengineering, please apply or share. We have a great institute with amazing colleagues and core facilities,highly recommend

application.mdc-berlin.de/site/index.p...

MDC Berlin - Online Application System

application.mdc-berlin.de

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Misha Kudryashev @mishakudryashev.bsky.social · 16d

It's a team effort of the group: Igor, Ulja, Xiaofeng, Artsemi, Patricia, and we thank the funders.

The print version will come up in a book on methods in molecular biology, hopefully later this year!

Enjoy reading and have a great week!

Misha Kudryashev @mishakudryashev.bsky.social · 16d

But for a larger system, smaller proteins, interactions with the cytoskeleton, cluster formation, and other biological applications, there seems to be a use of this microvesicle technique.

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Misha Kudryashev @mishakudryashev.bsky.social · 16d

Some thoughts: wonderful papers by Roderick Mackinon and other groups show that 2D imaging of membrane proteins in small vesicles can result in high-resolution structures. So if you want structures - try that!

pubmed.ncbi.nlm.nih.gov/37098056/

Membrane protein isolation and structure determination in cell-derived membrane vesicles - PubMed

Integral membrane protein structure determination traditionally requires extraction from cell membranes using detergents or polymers. Here, we describe the isolation and structure determination of pro...

pubmed.ncbi.nlm.nih.gov

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Misha Kudryashev @mishakudryashev.bsky.social · 16d

The book chapter is a set of methods to express your membrane protein of interest, purify vesicles, image them by cryo-ET, and perform subtomogram averaging.

As usual, we like to go into details:

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Misha Kudryashev @mishakudryashev.bsky.social · 16d

The chapter is inspired by our recent paper, where we show that tetrameric and pentameric forms of the serotonin receptor ion channel 5-HT3R were present in the same membrane patches
www.embopress.org/doi/full/10....

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Misha Kudryashev @mishakudryashev.bsky.social · 16d

We have a preprint of a book chapter for you: Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles.

We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography

zenodo.org/records/1717...

Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles

zenodo.org

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Misha Kudryashev @mishakudryashev.bsky.social · Sep 3

Roses are red
Because they have SCREP!

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Misha Kudryashev @mishakudryashev.bsky.social · Aug 12

I'd say it's a paper of the week if not a year

Lithium deficiency and the onset of Alzheimer’s disease
www.nature.com/articles/s41...

Lithium deficiency and the onset of Alzheimer’s disease - Nature

Lithium has an essential role in the brain and is deficient early in Alzheimer’s disease, which can be recapitulated in mice and treated with a novel lithium salt that restores the physiological level...

www.nature.com

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Misha Kudryashev @mishakudryashev.bsky.social · Aug 4

Postdoc program at the MDC in Berlin, @mdc-berlin.bsky.social : take a look 👇👇

For this call, collaborative projects between two groups will be funded. Take a look at the call and the groups and drop me an email if interested. Share with colleagues too!

www.mdc-berlin.de/postdocs#t-g...

www.mdc-berlin.de

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Misha Kudryashev @mishakudryashev.bsky.social · Jun 19

Building atomic models to cryo-EM maps of huge molecules is intimidating: crowded, uncertain copy numbers, variable resolution ...
Fan Liu and team incl us, show how cross-linking MS can be used to build novel models of unknown complexes, like a giant Melbourne virus
biorxiv.org/content/10.1...

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Misha Kudryashev @mishakudryashev.bsky.social · Jun 13

Thanks Mimi! ❄️❄️❄️

Misha Kudryashev @mishakudryashev.bsky.social · Jun 9

Thanks Sandip!

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Misha Kudryashev @mishakudryashev.bsky.social · Jun 9

Thanks John !!!

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Misha Kudryashev @mishakudryashev.bsky.social · Jun 8

We have a preprint for you

EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer

By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...

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Misha Kudryashev @mishakudryashev.bsky.social · May 19

It's fellowship season in the EU. If you want to talk and join our group at the amazing MDC in Berlin on a fellowship, please write me an email.

We do cryo-EM/ET, membrane proteins, data science/AI, our work: scholar.google.com/citations?so...

Fellowships: Marie Curie, EMBO, HFSP, Humboldt...

scholar.google.com

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Misha Kudryashev @mishakudryashev.bsky.social · May 15

Our contribution to Mohit Misra, Ivan Đikić, and Co's stellar research on how a pathogenic bacterium, Legionella pneumophila, hijacks host cell signaling

It was a huge effort and took many years to combine all the data and methods, so enjoy reading👇
www.researchsquare.com/article/rs-6...

Structural Basis for Membrane Targeting and Secretion of Legionella SidE Ubiquitin Ligases

SidE family effectors from Legionella pneumophila catalyze serine ubiquitination of host proteins via phospho-ribose (PR) linkages, disrupting host compartments, facilitating the formation of Legionel...

www.researchsquare.com

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Misha Kudryashev @mishakudryashev.bsky.social · May 6

I think that it is not compatible with Krios 5

Misha Kudryashev @mishakudryashev.bsky.social · May 2

yeah, its very sad that Thermo makes older devices non-compatible.

Thanks!

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Misha Kudryashev @mishakudryashev.bsky.social · Apr 29

Thanks !

Misha Kudryashev @mishakudryashev.bsky.social · Apr 28

Cool thanks Paulo. Any pros cons on arctis vs hydra ?

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Misha Kudryashev @mishakudryashev.bsky.social · Apr 28

Cool thanks Katie, we have a similar experience in house. It's a great working horse 🐴🐴🐴

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Misha Kudryashev @mishakudryashev.bsky.social · Apr 28

Hydra does look good on paper. Anything that could be improved in it ?

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Misha Kudryashev @mishakudryashev.bsky.social · Apr 28

Thanks ! Cool. Are you happy with the milling throughput ?

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Misha Kudryashev @mishakudryashev.bsky.social · Apr 28

Hi Cryo-EM fellas!

What are the pros and cons of the latest cryoFIB milling tools? Any usage experience with Arctis already? Any huge advantages of plasma?

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