Dmitry Sutormin
@monochamussutor.bsky.social
Scientist, molecular biologist and bioinformatician with a focus in microbiology
Reposted by Dmitry Sutormin
Pooled CRISPR screens with transcriptomics readout arrived for bacteria. We introduce mapSPLiT, an approach to profiling transcriptional responses to CRISPR perturbations at scale. Collaborative work with Carothers, Zalatan, and Seelig groups at [email protected] www.biorxiv.org/content/10.6... 1/4
Pooled single-cell CRISPRa/i screens for functional genomics in bacteria at scale
Pooled CRISPR screens using single-cell RNA sequencing (scRNA-seq) have emerged as powerful tools to uncover gene function, map regulatory networks, and identify genetic interactions. However, the inh...
www.biorxiv.org
December 23, 2025 at 7:42 PM
Pooled CRISPR screens with transcriptomics readout arrived for bacteria. We introduce mapSPLiT, an approach to profiling transcriptional responses to CRISPR perturbations at scale. Collaborative work with Carothers, Zalatan, and Seelig groups at [email protected] www.biorxiv.org/content/10.6... 1/4
Reposted by Dmitry Sutormin
The GTDB website now has an ANI calculator based on skani that supports uploading of user genomes. Try it at gtdb.ecogenomic.org/tools/skani.
Find more information about @jimshaw.bsky.social fantastic tool at www.nature.com/articles/s41....
Find more information about @jimshaw.bsky.social fantastic tool at www.nature.com/articles/s41....
GTDB - skani calculator
An interface to compute pairwise ANI of NCBI genomes using the GTDB taxonomy.
gtdb.ecogenomic.org
December 11, 2025 at 2:59 PM
The GTDB website now has an ANI calculator based on skani that supports uploading of user genomes. Try it at gtdb.ecogenomic.org/tools/skani.
Find more information about @jimshaw.bsky.social fantastic tool at www.nature.com/articles/s41....
Find more information about @jimshaw.bsky.social fantastic tool at www.nature.com/articles/s41....
Reposted by Dmitry Sutormin
It’s finally out!
Our paper on the bacterial symbionts of three White Sea sponges is here.
A story that began in 2016 has reached its last chapter… or has it? We hope that by the time you finish our hefty tome — maybe around 2034 — we’ll release the sequel.
journals.asm.org/doi/10.1128/...
Our paper on the bacterial symbionts of three White Sea sponges is here.
A story that began in 2016 has reached its last chapter… or has it? We hope that by the time you finish our hefty tome — maybe around 2034 — we’ll release the sequel.
journals.asm.org/doi/10.1128/...
Taxonomically different symbiotic communities of sympatric Arctic sponge species show functional similarity with specialization at species level | mSystems
Sponges are regarded among the earliest multicellular organisms and the most ancient
examples of animal-bacterial symbiosis. The study of host-microbe interactions in
sponges has advanced rapidly due ...
journals.asm.org
October 20, 2025 at 8:48 PM
It’s finally out!
Our paper on the bacterial symbionts of three White Sea sponges is here.
A story that began in 2016 has reached its last chapter… or has it? We hope that by the time you finish our hefty tome — maybe around 2034 — we’ll release the sequel.
journals.asm.org/doi/10.1128/...
Our paper on the bacterial symbionts of three White Sea sponges is here.
A story that began in 2016 has reached its last chapter… or has it? We hope that by the time you finish our hefty tome — maybe around 2034 — we’ll release the sequel.
journals.asm.org/doi/10.1128/...
Reposted by Dmitry Sutormin
Marine sponges dont reveal their secrets easily. Curious? Read the blog below by @sangerinstitute.bsky.social
🌊 Sponge genomes are helping unlock the secrets of aquatic symbioses — and thanks to @sangerinstitute.bsky.social, researchers now have access to 50+ genomes. Excited to see how these new samples help advance understanding of #AquaticSymbiosis. 🧬
Enter the absorbing world of sponges - Wellcome Sanger Institute Blog
Enter the absorbing world of sponges, the intricate animals that have evolved to inhabit all corners of our Earth’s waterways and oceans. Thanks to a worldwide collaboration of sponge scientists throu...
sangerinstitute.blog
July 10, 2025 at 8:29 PM
Marine sponges dont reveal their secrets easily. Curious? Read the blog below by @sangerinstitute.bsky.social
Reposted by Dmitry Sutormin
July 3, 2025 at 12:01 AM
Reposted by Dmitry Sutormin
After many years in the making, this story is finally published! In the updated manuscript on BREX DNA recognition, we reveal that modifying the system's specificity can surprisingly enhance anti-phage defense.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Molecular basis of foreign DNA recognition by BREX anti-phage immunity system - Nature Communications
BrxX methylase in complex with SAM cofactor mediates foreign DNA recognition by the BREX system. BrxX can be engineered to modify BREX specificity and enhance defense. BREX defense and methylatio...
www.nature.com
February 22, 2025 at 9:24 AM
After many years in the making, this story is finally published! In the updated manuscript on BREX DNA recognition, we reveal that modifying the system's specificity can surprisingly enhance anti-phage defense.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Dmitry Sutormin
Check it out! Three Arctic sponge species, six years of observation, and seven symbiotic bacteria—six of which belong to novel candidate genera. An odyssey that began in 2016 is now complete!
www.biorxiv.org/content/10.1...
#Sponges #Microbiome #Symbiosis #BacteriaSymbionts #Arctic
www.biorxiv.org/content/10.1...
#Sponges #Microbiome #Symbiosis #BacteriaSymbionts #Arctic
Taxonomically different symbiotic communities of sympatric Arctic sponge species show functional similarity with specialization at species level
Marine sponges harbor diverse communities of associated organisms, including eukaryotes, viruses, and bacteria. Sponge associated microbiomes contribute to the health of the host organisms by defendin...
www.biorxiv.org
March 21, 2025 at 4:45 AM
Check it out! Three Arctic sponge species, six years of observation, and seven symbiotic bacteria—six of which belong to novel candidate genera. An odyssey that began in 2016 is now complete!
www.biorxiv.org/content/10.1...
#Sponges #Microbiome #Symbiosis #BacteriaSymbionts #Arctic
www.biorxiv.org/content/10.1...
#Sponges #Microbiome #Symbiosis #BacteriaSymbionts #Arctic
Reposted by Dmitry Sutormin
How can single-cell transcriptomics profile phage infection in individual microbial cells? Check out our preprint with Anika Gupta, Norma Morella, Dmitry Sutormin & other authors, in collaboration with Georg Seelig (UW) and Neel Dey (Fred Hutch). www.biorxiv.org/content/10.1...
Combinatorial phenotypic landscape enables bacterial resistance to phage infection
Success of phage therapies is limited by bacterial defenses against phages. While a large variety of anti-phage defense mechanisms has been characterized, how expression of these systems is distribute...
www.biorxiv.org
January 16, 2025 at 2:26 AM
How can single-cell transcriptomics profile phage infection in individual microbial cells? Check out our preprint with Anika Gupta, Norma Morella, Dmitry Sutormin & other authors, in collaboration with Georg Seelig (UW) and Neel Dey (Fred Hutch). www.biorxiv.org/content/10.1...