Thomas Kroneis
@motoid.bsky.social
PI @MUG 🇦🇹
Rare Cell Analysis, #Microchimerism
Rare Cell Analysis, #Microchimerism
Reposted by Thomas Kroneis
We are repeating our EMBO Practical course for imaging-based spatial omics this June in Heidelberg. We will cover sample preparation, multiplexed IF (IBEX and Immuno-SABER), antibody-DNA conjugation, in situ sequencing, data analysis and visualization. Lots of fun and in-depth discussions!
Would you like to get a comprehensive introduction to the various methods of spatial-omics and in particular those of multiplex immunofluorescence and spatial transcriptomics?
Then apply for #EMBOSpatialOmics by 10 March here 👉 s.embl.org/iso25-01-bl
#microscopy #imageanalysis #spatialbiology
Then apply for #EMBOSpatialOmics by 10 March here 👉 s.embl.org/iso25-01-bl
#microscopy #imageanalysis #spatialbiology
March 3, 2025 at 4:32 PM
We are repeating our EMBO Practical course for imaging-based spatial omics this June in Heidelberg. We will cover sample preparation, multiplexed IF (IBEX and Immuno-SABER), antibody-DNA conjugation, in situ sequencing, data analysis and visualization. Lots of fun and in-depth discussions!
Reposted by Thomas Kroneis
Sometimes the most challenging cases seem to pop up on Friday when you’re on your own. We tried to collect some helpful points when dealing with these cases. @nephroneu.bsky.social @kathrineller.bsky.social @odlerb.bsky.social @ckj-era.bsky.social
Check out Nephro-Emergencies. doi.org/10.1093/ckj/...
It's Friday, 3 pm, and the phone rings ... A practical approach to common nephrology consultations – Part 1
Abstract. This practical approach highlights the importance of timely recognition and management of various nephrological emergencies. The featured conditi
doi.org
February 13, 2025 at 11:20 AM
Sometimes the most challenging cases seem to pop up on Friday when you’re on your own. We tried to collect some helpful points when dealing with these cases. @nephroneu.bsky.social @kathrineller.bsky.social @odlerb.bsky.social @ckj-era.bsky.social
Reposted by Thomas Kroneis
Have you been bothered that practically every #SpatialTranscriptomic analysis tool assumes data to be 2D? Well, you were right - overlapping cells are a really big problem. Check out our report on bioRxiv: 2D, or not 2D? Investigating Vertical Signal Integrity of Tissue Slices. t.ly/CQX8K
2D, or not 2D? Investigating Vertical Signal Integrity of Tissue Slices
Imaging-based spatially resolved transcriptomics can localise transcripts within cells in 3D. Cell segmentation precedes assignment of transcripts to cells and annotation of cell function. However, ce...
t.ly
February 8, 2025 at 9:43 AM
Have you been bothered that practically every #SpatialTranscriptomic analysis tool assumes data to be 2D? Well, you were right - overlapping cells are a really big problem. Check out our report on bioRxiv: 2D, or not 2D? Investigating Vertical Signal Integrity of Tissue Slices. t.ly/CQX8K
