Nature Reviews Molecular Cell Biology, Published online: 06 October 2025; doi:10.1038/s41580-025-00895-4Co-transcriptional mRNA maturation is a complex, multistep process. This Review focuses on how the development of long-read sequencing methods enabled investigating the timing, coordination and outcomes of alternative uses of transcription start sites, splice sites and polyadenylation sites and their disease implications.

Nature Reviews Molecular Cell Biology, Published online: 02 October 2025; doi:10.1038/s41580-025-00906-4Christina Termini highlights the importance of the identification of haematopoietic stem cell markers.

Nature Reviews Molecular Cell Biology, Published online: 01 October 2025; doi:10.1038/s41580-025-00910-8Colon stem cells expressing the surface markers NOX1 and NPY1R can give rise to colon cancer in mice.

Nature Reviews Molecular Cell Biology, Published online: 01 October 2025; doi:10.1038/s41580-025-00905-5The advent of laser-scanning confocal microscopy revolutionized cell biology, offering unprecedented resolution and depth and enabling scientists to visualize cellular structures in 3D.

Nature Reviews Molecular Cell Biology, Published online: 30 September 2025; doi:10.1038/s41580-025-00882-9Protein tyrosine phosphatases (PTPs) regulate cell signalling and contribute to a wide range of human diseases. This Review discusses mechanistic insights into PTP function and regulation, their physiological roles and the development of PTP-targeted therapeutics.

Nature Reviews Molecular Cell Biology, Published online: 29 September 2025; doi:10.1038/s41580-025-00899-0Endoplasmic reticulum exit sites (ERES) are specialized ER subdomains that regulate the export of secreted cargo. This Roadmap explores how ERES integrate biochemical and mechanical signals to coordinate trafficking and proposes a multidisciplinary strategy to investigate their function, including in disease.

Nature Reviews Molecular Cell Biology, Published online: 02 October 2025; doi:10.1038/s41580-025-00906-4Christina Termini highlights the importance of the identification of haematopoietic stem cell markers.

Nature Reviews Molecular Cell Biology, Published online: 01 October 2025; doi:10.1038/s41580-025-00910-8Colon stem cells expressing the surface markers NOX1 and NPY1R can give rise to colon cancer in mice.

Nature Reviews Molecular Cell Biology, Published online: 01 October 2025; doi:10.1038/s41580-025-00905-5The advent of laser-scanning confocal microscopy revolutionized cell biology, offering unprecedented resolution and depth and enabling scientists to visualize cellular structures in 3D.

Nature Reviews Molecular Cell Biology, Published online: 30 September 2025; doi:10.1038/s41580-025-00882-9Protein tyrosine phosphatases (PTPs) regulate cell signalling and contribute to a wide range of human diseases. This Review discusses mechanistic insights into PTP function and regulation, their physiological roles and the development of PTP-targeted therapeutics.

Nature Reviews Molecular Cell Biology, Published online: 29 September 2025; doi:10.1038/s41580-025-00899-0Endoplasmic reticulum exit sites (ERES) are specialized ER subdomains that regulate the export of secreted cargo. This Roadmap explores how ERES integrate biochemical and mechanical signals to coordinate trafficking and proposes a multidisciplinary strategy to investigate their function, including in disease.

Nature Reviews Molecular Cell Biology, Published online: 26 September 2025; doi:10.1038/s41580-025-00903-7A newly discovered hybrid protein modification of mono(ADP-ribose) and ubiquitin is recognized by dedicated effectors that promote processing of the modified proteins.

Nature Reviews Molecular Cell Biology, Published online: 24 September 2025; doi:10.1038/s41580-025-00878-5The development of a functional heart depends on the specification of multiple cell types, including pacemaker cells, and complex tissue re-organization. This Review discusses recent insights into these processes from in vivo studies and cardiac organoid models.

Nature Reviews Molecular Cell Biology, Published online: 26 September 2025; doi:10.1038/s41580-025-00903-7A newly discovered hybrid protein modification of mono(ADP-ribose) and ubiquitin is recognized by dedicated effectors that promote processing of the modified proteins.

Nature Reviews Molecular Cell Biology, Published online: 22 September 2025; doi:10.1038/s41580-025-00892-7To celebrate the journal’s 25th anniversary, we asked 13 researchers to offer a glimpse of what their research field might look like in 2050. They consider how technological breakthroughs — for example, artificial intelligence-powered virtual cells — could transform our understanding of how molecules, organelles and cells behave in different contexts, revolutionize therapies and enable the design of resilient crops.

Nature Reviews Molecular Cell Biology, Published online: 22 September 2025; doi:10.1038/s41580-025-00902-8A 2017 cryo-electron microscopy structure that resolved the orientation of the replicative helicase CMG at the DNA replication fork caused a major shift in our understanding of DNA replication.

Nature Reviews Molecular Cell Biology, Published online: 24 September 2025; doi:10.1038/s41580-025-00878-5The development of a functional heart depends on the specification of multiple cell types, including pacemaker cells, and complex tissue re-organization. This Review discusses recent insights into these processes from in vivo studies and cardiac organoid models.

Nature Reviews Molecular Cell Biology, Published online: 22 September 2025; doi:10.1038/s41580-025-00892-7To celebrate the journal’s 25th anniversary, we asked 13 researchers to offer a glimpse of what their research field might look like in 2050. They consider how technological breakthroughs — for example, artificial intelligence-powered virtual cells — could transform our understanding of how molecules, organelles and cells behave in different contexts, revolutionize therapies and enable the design of resilient crops.

Nature Reviews Molecular Cell Biology, Published online: 22 September 2025; doi:10.1038/s41580-025-00902-8A 2017 cryo-electron microscopy structure that resolved the orientation of the replicative helicase CMG at the DNA replication fork caused a major shift in our understanding of DNA replication.

Nature Reviews Molecular Cell Biology, Published online: 22 September 2025; doi:10.1038/s41580-025-00904-6Acute kidney injury induces somatic mitochondrial DNA mutations that impair energy metabolism and the resilience of kidney tissue to following injuries.