Natalie Niemi
@nieminm.bsky.social
Assistant professor @WUSTLmed Biochemistry & Molecular Biophysics. Mitochondria, metabolism, and cell signaling. Mom of 2. Advocate for women in science. www.niemilab.com
Can also look up how we made the stocks and send that info along if it’s helpful!
November 24, 2025 at 11:27 PM
Can also look up how we made the stocks and send that info along if it’s helpful!
Originally got this from a Methods book chapter that I can dig up if you need, but almost sure it was from Liza Pon’s group, and I copy/pasted the above components from this paper: pmc.ncbi.nlm.nih.gov/articles/PMC...
Isolation of mitochondria from Saccharomyces cerevisiae using magnetic bead affinity purification
Isolated mitochondria are widely used to study the function of the organelle. Typically, mitochondria are prepared using differential centrifugation alone or in conjunction with density gradient ultracentrifugation. However, mitochondria isolated ...
pmc.ncbi.nlm.nih.gov
November 24, 2025 at 11:25 PM
Originally got this from a Methods book chapter that I can dig up if you need, but almost sure it was from Liza Pon’s group, and I copy/pasted the above components from this paper: pmc.ncbi.nlm.nih.gov/articles/PMC...
(PI-1: 0.5-mg/ml Pepstatin A, 0.5 μg/ml Chymostatin, 0.5 μg/ml Antipain, 0.5 μg/ml Leupeptin, and 0.5 μg/ml Aprotinin; PI-2: 10 μM Benzamidine–HCl and 1 μg/ml 1,10-Phenanthroline; 1 mM PMSF)
November 24, 2025 at 11:25 PM
(PI-1: 0.5-mg/ml Pepstatin A, 0.5 μg/ml Chymostatin, 0.5 μg/ml Antipain, 0.5 μg/ml Leupeptin, and 0.5 μg/ml Aprotinin; PI-2: 10 μM Benzamidine–HCl and 1 μg/ml 1,10-Phenanthroline; 1 mM PMSF)
We used to use two homemade protease inhibitor cocktails when we would do yeast mito isolations, PI-1 and PI-2:
November 24, 2025 at 11:25 PM
We used to use two homemade protease inhibitor cocktails when we would do yeast mito isolations, PI-1 and PI-2: