banner
nogaleslab.bsky.social
Nogales Lab
@nogaleslab.bsky.social
1.7K followers 39 following 34 posts
The Nogales Lab @ UC Berkeley. #CryoEM #Transcription #Microtubules #ChromatinModification Bluesky account managed by lab members.
Posts Media Videos Starter Packs
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jul 19
A unique honor to become a Foreign Member of the @royalsociety.org, together with my @ucberkeleyofficial.bsky.social colleague Stuart Russell and old friends like John Briggs, Asia Akhtar, Patrick Cramer or Ruth Lehmann. Learning about great science and science history was a real treat.
1 3 45
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jul 19
Very grateful to the Menéndez Pelayo International University for awarding me an honorary doctorate last Tuesday, July 8th, in the incomparable setting of the Magdalena Palace (Santander). On Monday I enjoyed a conversation with those interested in my career and science.
6
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jul 7

Our work, co-led by @alfredo0712.bsky.social and @nlue8.bsky.social is out in @natsmb.nature.com Shout out to coauthors P.Grob, B. Kaeser, J.Fang, and S. Kassube for their contributions and thanks to the reviewers for the suggestions that led to the final work.
www.nature.com/articles/s41...
Structural insights into transcriptional regulation by the helicase RECQL5 - Nature Structural & Molecular Biology
Florez Ariza and Lue et al. use cryo-electron microscopy to investigate how the RECQL5 helicase regulates transcription. Their structural findings suggest that RECQL5 can modulate RNA polymerase II’s ...
www.nature.com
1 12 38
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jul 5
So proud of the smashing success of the International Course in Biophysics co-organized with my @berkeleymcb.bsky.social colleague Carlos Bustamante and University of Salamanca @usaloficial.bsky.social
biophysal.org
Course ends with a Keynote from Nobel Laureate Richard Henderson🥇
BioPhySAL - First Salamanca International Advanced Biophysics and Structural Biology Course
First Summer International Advanced Biophysics and Structural Biology Course. Eva Nogales - Carlos Bustamante.
biophysal.org
3 13
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jun 24
Yesterday I had the pleasure to visit the Institute for Material Science in Madrid @icmm-csic.bsky.social. Thank you to Dr. Pedro Serana for his invitation to talk about cryo-EM and our latest microtubule studies 🧩🔬🧬
6
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jun 24
I spent a wonderful day in the Universidad de Córdoba at an interactive breakfast with faculty and students and giving a science presentation. Lovely to meet its Chancellor, Manuel Torralbo, and my friend and past collaborator Prof. Tina Domínguez-Martín.
2
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jun 24
It was an absolute pleasure to visit the IBiS, to meet its scientists, interact with its students, and visit beautiful Sevilla 🥰
ibis-investigacion.bsky.social
IBiS - Instituto de Biomedicina de Sevilla @ibis-investigacion.bsky.social · Jun 17
🟠SCIENCE |

Thank you very much to Prof. Eva Nogales for sharing her outstanding and inspiring science with us. It was an honor to host her at IBiS!

@hhmi.org @unisevilla.bsky.social @csic.es @nogaleslab.bsky.social @csicandalextrem.bsky.social @ucberkeleyofficial.bsky.social
1 1
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jun 24
Excited to have participated in the Third Symposium of Physical Chemistry in the Universidad Autónoma de Madrid @uam.es last week and honored that the Prizes for best student talk and best posters carried my name 🥰☺️
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jun 24
Que día tan emocionante volver a mi colegio en Colmenar Viejo y recibir el calor de sus profesores y sus alumnos. Gracias por darle mi nombre a vuestra biblioteca! 🥰
1 2
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jun 24
Deeply touched and honored by the Universidad Autónoma de Madrid, my alma mater, (@uam.es) making me Doctor Honoris Causa. Thank you to the Department of Physical Chemistry for nominating me for this recognition. It was a memorable day for me and my family👩‍🎓
3 40
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jun 24
Like a dream, my name next to that of @isabelallende.com, and to be recognized by Universidad Internacional Menéndez Pelayo UIMP. Soon after, Irene Vallejo @irenevalmore.bsky.social will receive the same honor. I feel proud to be linked to these truly inspiring, talented women! Thank you UIMP!
1 2 14
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · May 23
This week our lab had a double celebration: 1) Eva’s election into the @royalsociety.org and 2) Zhenlin’s new job in Southern University of Science and Technology, China. He will be missed but we wish him success and joy in his independent career!
1 10
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · May 8
Check out our review article on the structural and functional studies of retrotransposon proteins by @akankshathawani.bsky.social together with Kathy Collins' lab.

www.sciencedirect.com/science/arti...
Structural and biochemical studies of mobile retrotransposon proteins in action
Autonomous non-long-terminal repeat (non-LTR) retrotransposons, including long interspersed elements (LINEs), are mobile genetic elements abundant in …
www.sciencedirect.com
4 5
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jan 31
A huge shoutout to our former grad student Susanne Kassube (now PI) for leading our lab’s original study back in 2013 and setting the stage for this work. If you’re interested, check out our preprint for more details and stay tuned for the full paper! 7/7
4
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jan 31
Beyond a role in slowing down Pol II elongation, RECQL5 is known to decrease transcription stress. We propose that RECQL5 may help restart transcription from stalled Pol II by allosterically modulating Pol II’s translocation state. 6/7
1 1 1
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jan 31
Nucleotide binding (AMPPNP or ADP) causes the RECQL5 helicase domain to undergo a conformational change, pulling on the DNA to induce Pol II to shift to a post-translocation state. Meanwhile, the IRI module seems to serve as a stable anchor for RECQL5 binding. 5/7
1
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jan 31
The high resolution of our structures also enabled us to ask how RECQL5 affects Pol II, in particular its translocation state on a DNA/RNA scaffold. We found that Pol II occupies different translocation states when RECQL5 helicase is in its apo vs. nucleotide-bound form. 4/7
1
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jan 31
We report high-resolution structures of Pol II elongation complexes with RECQL5, visualizing interactions between RECQL5, Pol II, and DNA, confirming its role as a transcriptional roadblock. This required extensive data processing, as RECQL5 is very flexible! 3/7
1
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jan 31
RecQ helicases help safeguard genome stability. In this family, RECQL5 stands out as the only one to interact with and inhibit RNA Pol II, and it’s been shown to help decrease transcription stress. We used cryo-EM to investigate the molecular basis for this mechanism. 2/7
1
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jan 31
Check out our lab’s latest work on the regulation of transcription by RECQL5 helicase! This work was co-led by @alfredo0712.bsky.social and @nlue8.bsky.social, with contributions from Patricia Grob, Ben Kaeser, Jie Fang, and Susanne Kassube. 1/7
www.biorxiv.org/content/10.1...
Structural insights into transcriptional regulation by the helicase RECQL5
Transcription and its regulation pose a major challenge for genome stability. The helicase RECQL5 has been proposed as an important factor to help safeguard the genome, and is the only member of the h...
www.biorxiv.org
1 14 46
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jan 30
The recording of the talks is now available at youtube.com/playlist?lis...
Cryo-EM: The Next 50 Years Symposium - YouTube
January 11, 2025 - UC Berkeley
youtube.com
17 34
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jan 16
I encourage you to apply and join us at @ucberkeleyofficial.bsky.social !
jimhurley.bsky.social
Jim Hurley @jimhurley.bsky.social · Jan 15
Scientific Director, Cal-Cryo. We are looking for an outstanding microscopist to run our cryo-EM facility (two Krioses and an Arctica), increase training, engagement, and collaboration on campus, expand our user base, and join our community of cutting edge cryo-EM researchers. tinyurl.com/ykasna3p
Scientific Director, Cal-Cryo@QB3, UC Berkeley’s Cryo-EM Facility
University of California, Berkeley is hiring. Apply now!
tinyurl.com
4 5
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jan 12
Our microtubule team at lunch with @ruizhangmt.bsky.social, who gave a spectacular seminar at UC Berkeley on his work on axonemes
8
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jan 12
The Cryo-EM: the next 50 years Symposium held yesterday at @ucberkeleyofficial.bsky.social was a smashing success!!
3 16 82
nogaleslab.bsky.social
Nogales Lab @nogaleslab.bsky.social · Jan 8
These two distinct inhibition mechanisms are determined by the unique location of H3K4me3 and H3K36me3 along the histone H3 tail and target two essential requirements for efficient trimethylation of histone H3K27 to exclude PRC2 activity from actively transcribe genes