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piatkevich.bsky.social @piatkevich.bsky.social · 17d

#OpenCloning opencloning.org now features WeKwikGene integration—streamlining downloading plasmid sequences from wekwikgene.wllsb.edu.cn and downstream cloning and collaborations!

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piatkevich.bsky.social @piatkevich.bsky.social · 22d

Fluorescent protein-based ticker tapes (FPTT) record transcriptional histories of multiple promoters on single cellular fibers. Quantitative readout is available at any time point. Cells become biological archives of their own gene expression biorxiv.org/content/10.1...

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piatkevich.bsky.social @piatkevich.bsky.social · 24d

Major milestone for WeKwikGene! wekwikgene.wllsb.edu.cn now features 1,000+ ready-to-ship plasmids with 800+ more plasmids under QC. We've processed 1,000+ requests & distributed 3,000+ plasmids worldwide.

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piatkevich.bsky.social @piatkevich.bsky.social · 29d

Here is the link to the original study by Satoshi Sawai www.micropublication.org/journals/bio...

Use of blue fluorescent protein Electra2 for live-cell imaging in Dictyostelium discoideum | microPublication

www.micropublication.org

piatkevich.bsky.social @piatkevich.bsky.social · 29d

Our blue FP Electra2 shows excellent brightness & localization in soil-dwelling amoeba D. discoideum as fusion marker, with performance comparable to the golden standard mTagBFP2, all plasmids for Electra2 are available from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

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piatkevich.bsky.social @piatkevich.bsky.social · Sep 9

The hCRAFi-CCR2 sensor reports chemokine release in the mouse visual cortex after LPS challenge using two-photon microscopy www.nature.com/articles/s41.... Plasmids and corresponding test AAVs can be requested from WeKwikGene for free wekwikgene.wllsb.edu.cn/publications...

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piatkevich.bsky.social @piatkevich.bsky.social · Jul 25

hCRAFi-CCR2 is a new chemokine receptor activation-based fluorescent indicator for monitoring spatiotemporal dynamics of CCR2 ligands, including human/mouse CCL2, and CCL7, as well as mouse CXCL1, in culture and in vivo www.nature.com/articles/s41...

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piatkevich.bsky.social @piatkevich.bsky.social · Jul 4

PSmOrange3 works well for long-term mitochondria tracking in cultured mammalian cells. Plasmids for PSmOrange3 can be requested for free from @WeKwikGene here wekwikgene.wllsb.edu.cn/publications...

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piatkevich.bsky.social @piatkevich.bsky.social · Jul 2

Temporal domain multiplexing enables multiplexed imaging in a single channel with spectrally similar FPs using per-pixel unmixing based on differences in photobleaching rates. To facilitate this approach, we developed new far-red FPs www.biorxiv.org/content/10.1...

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piatkevich.bsky.social @piatkevich.bsky.social · Jun 18

mScarlet3-H (aka mYongHong) is on the cover of Nature Methods! All plasmids are available for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

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piatkevich.bsky.social @piatkevich.bsky.social · Jun 14

Here is a link to apply www.piatkevich-lab.com/join-us

Join us | Labsite

www.piatkevich-lab.com

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piatkevich.bsky.social @piatkevich.bsky.social · Jun 10

Using mBaoJin derivative, Peng Zou lab engineered highly photostable green eFRET GEVI, named Vega, which exhibits more than 20-fold slower photobleaching than Ace-mNeon2, and allows for long-term voltage imaging in brain slice and in vivo in mice biorxiv.org/content/10.1...

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piatkevich.bsky.social @piatkevich.bsky.social · Jun 7

to me it was like "try to do as little as possible for as high as possible impact"

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piatkevich.bsky.social @piatkevich.bsky.social · Jun 7

Haha, I remember that Ed said each of these to me many times, as well as "your success depends not on the things you do, but often on things you do not do", now I use them in my group!

piatkevich.bsky.social @piatkevich.bsky.social · Jun 5

Xue Han lab from BU used ElectraOFF to achieve routine cellular-resolution imaging over tens of minutes, occasionally up to eighty minutes, in behaving mice with minimal signal loss across neuron types and brain regions www.biorxiv.org/content/10.1...

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piatkevich.bsky.social @piatkevich.bsky.social · Jun 4

Hour-long voltage imaging in behaving mice! Capitalizing on the high photostability and brightness of mBaoJin, we developed eFRET GEVIs, named ElectraOFF, which extends reliable single spike detection at cellular resolution beyond one hour biorxiv.org/content/10.1...

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piatkevich.bsky.social @piatkevich.bsky.social · May 20

Due to its high chemical stability and brightness, mBaoJin is a perfect FP for Expansion Microscopy. Three-dimensional view of expanded HeLa cell coexpressing H2B-mTurquoise2 and Mito (COX8Ax2)-mBaoJin (www.nature.com/articles/s41...)

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piatkevich.bsky.social @piatkevich.bsky.social · May 19

Soma-targeting of GECIs significantly improves image segmentation, as can be seen from these examples of single-plane 2-photon imaging for FRCaMPi, jRGECO1a, and their soma-localized variant from L2/3 neurons of primary visual cortex in awake mouse journals.plos.org/plosbiology/...

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piatkevich.bsky.social @piatkevich.bsky.social · May 8

mScarlet3-H (aka mYongHong) makes a great pair with mBaoJin (nature.com/articles/s41...) for long-term live cell imaging. All plasmids can be requested for free from WeKwikGene wekwikgene.wllsb.edu.cn

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piatkevich.bsky.social @piatkevich.bsky.social · May 8

A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution in mice and zebrafish journals.plos.org/plosbiology/...

piatkevich.bsky.social @piatkevich.bsky.social · May 3

All plasmids for FRCaMPis and SomaFRCaMPi are avalible from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

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piatkevich.bsky.social @piatkevich.bsky.social · Apr 21

Great News & Views article by Ben Campbell covering recent development in FP field with focus on mScralet3-H (aka mYongHong) www.nature.com/articles/s41...

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piatkevich.bsky.social @piatkevich.bsky.social · Apr 18

mScarlet3-H (aka mYongHong), a highly stable monomeric red fluorescent protein, works well for long-term super-resolution imaging, rapid tissue clearing, and CLEM www.nature.com/articles/s41...

A highly stable monomeric red fluorescent protein for advanced microscopy - Nature Methods

The red fluorescent protein mScarlet3-H is bright, photostable and very robust to high temperature, chaotropic conditions and oxidative environments. mScarlet3-H works well in correlative light and el...

www.nature.com

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piatkevich.bsky.social @piatkevich.bsky.social · Apr 2

We are very happy to see another orange flame lit up for our Electra2 plasmids on Addgene! Btw these plasmids can be also requested for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

piatkevich.bsky.social @piatkevich.bsky.social · Feb 25

New applications of Electra2! Patel et al. used Electra2 to screen for genetically modified nematodes to study dopamine signaling driving skin invasion (www.biorxiv.org/content/10.1...). All plasmids for Elctras can be requested from WeKwikGene wekwikgene.wllsb.edu.cn/publications...

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