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Reposted by Magnus Kjærgaard

idpgrc.bsky.social @idpgrc.bsky.social · 6d

🚨🚨The GRC on Intrinsically Disordered Proteins #IDPs is back!! 🍝🧵🏔️Join us for cutting-edge IDP science, great discussions, and mountain views 🌄🍝
📅 June 21-26, 2026 | Les Diablerets, Switzerland
👉 Details & registration: grc.org/intrinsicall...

2026 Intrinsically Disordered Proteins Conference GRC

The 2026 Gordon Research Conference on Intrinsically Disordered Proteins will be held in Les Diablerets, Vaud (fr) Switzerland. Apply today to reserve your spot.

grc.org

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Magnus Kjærgaard @proteinmagnus.bsky.social · 9d

Would you like to hear about our work developing a screening pipeline for de novo designed binders us the microfluidic sizing?

Then tune in on Thursday for a virtual seminar. Sign-up below:

fidabio.webinargeek.com/how-to-scree...

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Magnus Kjærgaard @proteinmagnus.bsky.social · 23d

As FIDA relies on changes in hydrodynamics radius, it works best for small targets such as motifs from IDPs.

Luckily, this is what we intended to use it for 😉

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Magnus Kjærgaard @proteinmagnus.bsky.social · 23d

Crucial to the screening is the lab scale protein expression pipeline below.

We use an old trick beloved by the IDR-community for protein purication: Heat lysis.

Due the thermostability of the binders, we get ~95% pure lysate after a heating step in 96-well plates.

dx.doi.org/10.17504/pro...

Medium throughput protein expression and purification for protein design using heat lysis

This protocols describes medium throughput protein expression in E. coli using heat-lysis as a built in purification step. It is intended for preparation of heat-stable de novo...

dx.doi.org

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Magnus Kjærgaard @proteinmagnus.bsky.social · 23d

Experimental verification is increasingly the bottleneck in protein design projects.

In this paper, we develop a new mini-binder screening pipeline based on the microfluidic sizing technique called flow-induced dispersion analysis (FIDA).

doi.org/10.1002/pro....

Screening de novo designed protein binders in unpurified lysate using flow induced dispersion analysis

Computational protein design can create binders against targets of interest, but identifying binders with sufficient affinity still requires biochemical screening of many designs. In this work, we te....

doi.org

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Magnus Kjærgaard @proteinmagnus.bsky.social · Jun 19

The technique works best for small targets with a large change in radius of hydration upon binding – e.g. SLiMs in IDPs 😊 – but also works for larger proteins with only a 10% change in RH upon binding.

Magnus Kjærgaard @proteinmagnus.bsky.social · Jun 19

We find that it works excellently with “boiling lysis” – exploiting the high thermostability of binder designs to remove endogenous proteins – and then simply dilution the supernatant with buffer before measurement.

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Magnus Kjærgaard @proteinmagnus.bsky.social · Jun 19

FIDA measure radius of hydration based on diffusion in a microfluidic capillary.

This allows us to pick out successful binders based on the increase in R_H – a one-point measurement for initial screening and titration for affinity determination.

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Magnus Kjærgaard @proteinmagnus.bsky.social · Jun 19

How do we screen do novo designed binders most efficiently? In this pre-print, we show that the microfluidic sizing technique FIDA allows us to pick successful binders in a single measurement using minimal sample:

www.biorxiv.org/cgi/content/...

www.biorxiv.org

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Reposted by Magnus Kjærgaard

IDPSeminars @idpseminars.bsky.social · Jun 3

IDPSeminars is back with another seminar this week from @sarahshammas.bsky.social and @sonia-longhi.bsky.social!

Come hear about transcriptional regulation and viral pathogenesis!

Thursday June 5th!
PST 10 am EST 1 pm CET 7 pm!

Info: idpseminars.com
Signup form: forms.gle/3yCHeYvuuqLh...

Sarah Shammas (University of Oxford):Opportunities in DNA binding provided by protein disorder

Sonia Longhi (AFMB Marseille): Intrinsic disorder, phase transitions and fibril formation by the Henipavirus V and W proteins: a new hallmark of viral pathogenesis?

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Reposted by Magnus Kjærgaard

Ian Dunt @iandunt.bsky.social · May 30

Reactionaries hate universities because they create open-minded liberated people open.substack.com/pub/iandunt/...

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Reposted by Magnus Kjærgaard

DANDRITE @dandrite.bsky.social · May 26

Our annual report 2024 is out!
Dive into our research highlights, talent development, collaborations, and new events and initiatives.

dandrite.au.dk/annual-repor...

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Magnus Kjærgaard @proteinmagnus.bsky.social · May 21

🚨We have an opening for a postdoc to use protein design techniques to developing and characterizing binders against intrinsically disordered proteins involved in neuroscience.

Experience in protein biochemistry and/or molecular neuroscience preferred.

Share: 🙏

mbg.au.dk/aktuelt/ledi...

Postdoc: De novo protein design for neuroscience - Ledig stilling på Aarhus Universitet

Ledig stilling ved Institut for Molekylærbiologi og Genetik - Neurobiologi, Aarhus Universitet

mbg.au.dk

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Magnus Kjærgaard @proteinmagnus.bsky.social · May 17

The most wholesome Fox News show: 5 fox cubs playing in our garden.

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Reposted by Magnus Kjærgaard

Peter Andersen @germline.bsky.social · May 16

📣 🥳 🧑‍💻 Our department is hiring:

Tenure-Track Assistant Professor / Associate Professor in Bioinformatics at Aarhus University, Denmark

Deadline June 1st

Thanks for sharing!

mbg.au.dk/en/news-and-...

Tenure-Track Assistant Professor / Associate Professor in Bioinformatics at Aarhus University, Denmark - Vacancy at Aarhus University

Vacancy at Department of Molecular Biology and Genetics - BiRC - Bioinformatics Research Center, Aarhus University

mbg.au.dk

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Magnus Kjærgaard @proteinmagnus.bsky.social · May 15

Baby fox in the garden!

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Magnus Kjærgaard @proteinmagnus.bsky.social · May 13

Trigger warning: FOMO

How often do you go to a conference where you are excited about EVERY SINGLE TALK! Well, I guess it helps if you get to make the program. 😀

"Biomolecular condensates: From fundamental principles to applications in health and biotech" kicking off at 9 AM!

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Magnus Kjærgaard @proteinmagnus.bsky.social · May 12

Probably the best conference venue in Denmark?

Tomorrow, we kick-off our condensate meeting in the Carlsberg Academy ⬇️.

Thank you to the Novo Nordisk Foundation for the grant to host our dream meeting, and to the Carlsberg Foundation for hosting us at this beautiful site.

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Magnus Kjærgaard @proteinmagnus.bsky.social · Apr 8

We have a new review/perspective on the potential of biomolecular condensates in microbial cell factories. The emphasis is on the trade-offs that have to be navigated when designing such synthetic organelles.

Alain André @alain-andre.bsky.social · Apr 8

🚀Excited to share our latest review in Adv Biol!

Discover how biomolecular condensates enhance biochemical processes and their potential in biotech.
Shout out to Nikita, Ankush, and Magnus for making this possible.🌟

doi.org/10.1002/adbi...

@proteinmagnus.bsky.social & @nikitarehnberg.bsky.social

Toward Design Principles for Biomolecular Condensates for Metabolic Pathways

Biomolecular condensates are membrane-less compartments found through-out nature which can serve as reaction crucibles for biochemical processes. This review explores the design strategies underlying...

doi.org

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Magnus Kjærgaard @proteinmagnus.bsky.social · Mar 29

Since the Trump team are making videos about US-Greenland history, maybe the next one could be about that time when USAF crashed a B-52 spilling four nuclear bombs - one of which his probably never been recovered:
en.m.wikipedia.org/wiki/1968_Th...

1968 Thule Air Base B-52 crash - Wikipedia

en.m.wikipedia.org

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Reposted by Magnus Kjærgaard

Lars Løkke Rasmussen @lars-loekke.bsky.social · Mar 28

Dear American friends.

We agree that status quo in the Artcic is not an option.

So let’s talk about how we can fix it - together.

Lars Løkke Rasmussen
Danish Foreign Minister

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Magnus Kjærgaard @proteinmagnus.bsky.social · Mar 19

The bulk of the prize comes in the form of a grant for educational development.

We plan to use this to integrate computational skills - mainly python and R - across the whole curriculum. The prize allows us to hire someone the spearhead this across all the courses in our department!

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Magnus Kjærgaard @proteinmagnus.bsky.social · Mar 19

This is part of a new series of teaching prizes spanning from primary school to university level by the Novo Nordisk Foundation.

Seen from the outside, it looks like a 💰-push to increase the public and institutional recognition of teaching. Interesting to see how this will evlve.

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Magnus Kjærgaard @proteinmagnus.bsky.social · Mar 19

Surprised - in a good way - to find myself as a recipient of a big teaching national prize within Natural Science together with good colleagues @ditlevbrodersen.bsky.social and Gregers Rom Andersen.

nat.au.dk/en/about-the...

Three Educators from MBG Receive the Novo Nordisk Foundation Prize for Natural Science Teachers at Universities

Magnus Kjærgaard, Gregers Rom Andersen, and Ditlev Egeskov Brodersen teach biochemistry and structural biology to students at the Department of Molecular Biology and Genetics. Now, they are receiving ...

nat.au.dk

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Reposted by Magnus Kjærgaard

IDPSeminars @idpseminars.bsky.social · Mar 5

IDPSeminars is back this week (Tomorrow, March 6th)! Come hear about nucleolar organization and IDRs in host-pathogen interactions!

PST 10 am
EST 1 pm
CET 7 pm!

Info: idpseminars.com
Signup form: forms.gle/3yCHeYvuuqLh...

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