In this Perspective, we provide an overview of the core concepts around surface-enhanced Raman spectroscopy (SERS) enhancement factors (EFs), including both theoretical and experimental considerations...

Biochemical analysis of living systems such as single cells benefits greatly from the label-free and low-invasive molecular mapping with Raman microspectroscopy. Sets of Raman spectra at different spatial points are analyzed to generate Raman molecular maps corresponding to specific chemical species. However, human error and subjective data analysis can be technical issues that limit interpretation and its validity. Here, we present an objective data analysis scheme for postprocessing large data sets of Raman spectra for molecular mapping of living cells. The process comprises three steps: (i) Denoising the spectral data set using low-rank approximation; (ii) obtaining an objective background from data points outside the target cell; (iii) subtracting the thus obtained background using Hypothetical Addition Multivariate Analysis with Numerical Differentiation (HAMAND) via an automatically determined coefficient. Through the present analysis, minor Raman peaks, as indiscernible as they are, can be identified and precisely mapped. We demonstrate a quantitative discussion of cellular components after extracting contributions only from a single target cell from a Raman mapping image where multiple cells or parts of other cells are present. This work opens an improved analysis workflow for accurate spectroscopic analysis of living cells with the advantage of identifying minor Raman peaks unambiguously.

Raman spectroscopy is employed to show simultaneous, label-free imaging of RNA, DNA, and proteins, revealing secondary structural features of intracellular LLPS organelles, and condensed chromosome.

Raman spectroscopy is a robust, non-destructive analytical technique that offers detailed insights into the chemical composition, molecular structure,…

Lateral resolution is a key performance parameter for confocal Raman spectroscopy. We propose a protocol using an indium arsenide nanowire reference sample to quantitatively measure the resolution. T....

Quantification of titanium dioxide (TiO2) anatase and rutile polymorphs in binary mixtures by Raman spectroscopy: an interlaboratory comparison, Sacco, Alessio, Mandrile, Luisa, Tay, Li-Lin, Itoh, Nobuyasu, Raj, Ankit, Moure, Alberto, Del Campo, Adolfo, Fernandez, Jose F, Paton, Keith R, Wood, Sebastian, Kwon, Hyuksang, Adel, Tehseen, Hight Walker, Angela R, Martins Ferreira, Erlon Henrique, Theissmann, Ralf, Koch, Thomas, Giovannozzi, Andrea Mario, Portesi, Chiara, Rossi, Andrea Mario

This review describes current progresses of Raman spectroscopy in characterizing and imaging living eukaryotic cells, bacteria, and viruses. The Raman spectrum, specific to different types of unicell...

The market of gas sensors is mainly governed by electrochemical, semiconductor, and non-dispersive infrared absorption (NDIR)-based optical sensors. Despite offering a wide range of detectable gases, ...

Animal blood and semen analysis plays a significant role in national biological resource management, wildlife conservation, and customs security quarantine. Traditional blood analysis methods have dis...

A novel method for the accurate measurement of the dimensions of the focal volumes of Raman microscopes, using the edge of a graphene flake, was devised. In this work, it is explained in detail and a...

Scientific Reports - When cells divide: Label-free multimodal spectral imaging for exploratory molecular investigation of living cells during cytokinesis

Advances in the understanding of Raman processes in graphene have made it an essential tool for studying the properties of this one-atom-thick carbon material.

Subscription and open access journals from Sage, the world's leading independent academic publisher.

Subscription and open access journals from Sage, the world's leading independent academic publisher.

The sections in this article are

A comprehensive study of 35 naturally occurring lipids measured by Raman spectroscopy is presented. Additionally, we investigated the polymorphism/polytypism of triacylglycerols and analyzed the degr....