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Reposted by Rémi Hocq, PhD

Bart Pander @bartpander.bsky.social · Jul 4

We cannot have enough hot acetogens. : Revisiting carbon monoxide metabolism in the Moorella genus and isolation of a novel Moorella species,
Systematic and Applied Microbiology www.sciencedirect.com/science/arti...

Revisiting carbon monoxide metabolism in the Moorella genus and isolation of a novel Moorella species

Thermophilic acetogens hold great potential for syngas fermentation, as they efficiently convert CO and H2/CO2 into added-value chemical building bloc…

www.sciencedirect.com

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Rémi Hocq, PhD @remihocq.bsky.social · May 14

We evolved T. kivui to grow fast on CO (doubling time ~2.8 h). The trick? A megatransposon rewired its redox and metabolic circuits.
#Transposons: not just genetic noise, but engines of innovation!
Full story: doi.org/10.1038/s414...

#Biotechnology #Extremophiles #MetabolicEngineering

A megatransposon drives the adaptation of Thermoanaerobacter kivui to carbon monoxide - Nature Communications

Carbon monoxide (CO) present in syngas severely inhibits growth of many acetogens. Here, the authors adapt, characterize, and engineer utilization of CO as sole carbon and energy source by the thermop...

doi.org

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Rémi Hocq, PhD @remihocq.bsky.social · May 12

Our work opens the door to real #MetabolicEngineering in T. kivui.

It grows fast on CO₂ (∼2 h doubling time) with high carbon efficiency—ideal for sustainable fuel and chemical production.

With rapid, iterative genome editing now possible, these applications are within reach!

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Rémi Hocq, PhD @remihocq.bsky.social · May 12

✨ New publication! ✨

We made genome editing in Thermoanaerobacter kivui fast, reliable—and even cloning-free—by harnessing its own CRISPR system.

A clean edited strain can now be obtained in just 12 days!

Full story: doi.org/10.1186/s130...

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#CRISPR #SyntheticBiology #Extremophiles

Hi-TARGET: a fast, efficient and versatile CRISPR type I-B genome editing tool for the thermophilic acetogen Thermoanaerobacter kivui - Biotechnology for Biofuels and Bioproducts

Background Due to its ability to grow fast on CO2, CO and H2 at high temperatures and with high energy efficiency, the thermophilic acetogen Thermoanaerobacter kivui could become an attractive host fo...

doi.org

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Rémi Hocq, PhD @remihocq.bsky.social · Mar 11

Not sure you need any, PCR without the Ori with or without recircularization, and voilà ! But sacB is for sure a great approach ;)

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Rémi Hocq, PhD @remihocq.bsky.social · Mar 11

Looks cool! Was actually wondering: if you had a non-replicative plasmid, you could perhaps save tons of time?

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