@remixcoux.bsky.social
Ça fait longtemps qu'elle est juste islamophobe FBB
January 2, 2026 at 4:03 PM
Ça fait longtemps qu'elle est juste islamophobe FBB
Qu'attendre d'un édito de quatremer si ce n'est de la médiocrité?
December 18, 2025 at 8:20 AM
Qu'attendre d'un édito de quatremer si ce n'est de la médiocrité?
Planquez les mousses de micros!!
December 17, 2025 at 9:48 AM
Planquez les mousses de micros!!
Forgot the ref of Niwa's paper doi: 10.1038/74199
December 12, 2025 at 11:55 PM
Forgot the ref of Niwa's paper doi: 10.1038/74199
So... GATA6 takes advantage of the cis-landscape of DNA motifs and pre-established chromatin states, as well as the trans-compendium of available pioneers (including those supporting pluripotency), to reconfigure gene networks and trigger differentiation. I let you explore all the other details...
December 12, 2025 at 11:42 PM
So... GATA6 takes advantage of the cis-landscape of DNA motifs and pre-established chromatin states, as well as the trans-compendium of available pioneers (including those supporting pluripotency), to reconfigure gene networks and trigger differentiation. I let you explore all the other details...
Oh and we identified a region ~65kb upstream of Gata6, bound early in the conversion by GATA6/SOX17 and where OCT4/SOX2 are ectopically recruited. It exhibits reduced GATA6 binding in Oct4 kd cells suggesting that OCT4 reinforces GATA6 autoregulatory loop…
December 12, 2025 at 11:40 PM
Oh and we identified a region ~65kb upstream of Gata6, bound early in the conversion by GATA6/SOX17 and where OCT4/SOX2 are ectopically recruited. It exhibits reduced GATA6 binding in Oct4 kd cells suggesting that OCT4 reinforces GATA6 autoregulatory loop…
Endogenous Gata6 was also downregulated by Oct4 kd. We resorted to chemical PrE induction in the OCT4-AID line generated by Lawrence Bates and Jose Silva. Depleting OCT4 at differentiation onset resulted in massive cell death, confirming OCT4 requirement x PrE specification.
December 12, 2025 at 11:39 PM
Endogenous Gata6 was also downregulated by Oct4 kd. We resorted to chemical PrE induction in the OCT4-AID line generated by Lawrence Bates and Jose Silva. Depleting OCT4 at differentiation onset resulted in massive cell death, confirming OCT4 requirement x PrE specification.
Oct4 kd upon GATA6 induction decreased conversion efficiency, downregulated genes upregulated early in the ctrl conversion, and reduced GATA6/SOX17 binding at sites bound by PTFs (but increased binding at sites lacking PTFs).
December 12, 2025 at 11:38 PM
Oct4 kd upon GATA6 induction decreased conversion efficiency, downregulated genes upregulated early in the ctrl conversion, and reduced GATA6/SOX17 binding at sites bound by PTFs (but increased binding at sites lacking PTFs).
Considering all the data pointing towards pluripotency TF facilitating PrE specification- starting with Niwa’s seminal work showing that oct4 OE converts ESCs into XENs (but also work of Oren Ram, the Brickman lab and others…), I knocked down Oct4 during GATA6 induction…
December 12, 2025 at 11:38 PM
Considering all the data pointing towards pluripotency TF facilitating PrE specification- starting with Niwa’s seminal work showing that oct4 OE converts ESCs into XENs (but also work of Oren Ram, the Brickman lab and others…), I knocked down Oct4 during GATA6 induction…
SOX17 is rapidly activated by GATA6, but OCT4 and SOX2 are repurposed for differentiation
Many (if not all available pioneer TFs) cooperating to license new enhancers!!!
Many (if not all available pioneer TFs) cooperating to license new enhancers!!!
December 12, 2025 at 11:37 PM
SOX17 is rapidly activated by GATA6, but OCT4 and SOX2 are repurposed for differentiation
Many (if not all available pioneer TFs) cooperating to license new enhancers!!!
Many (if not all available pioneer TFs) cooperating to license new enhancers!!!
This is just an example: top, initial nucleosome states; bottom, final state. In black, regions binding GATA6 alone; in orange sites with pluripotency TFs at d0 but also recruiting SOX17/OCT4/SOX2 during differentiation; in red sites with no PTF at d0 but recruiting everybody!
December 12, 2025 at 11:37 PM
This is just an example: top, initial nucleosome states; bottom, final state. In black, regions binding GATA6 alone; in orange sites with pluripotency TFs at d0 but also recruiting SOX17/OCT4/SOX2 during differentiation; in red sites with no PTF at d0 but recruiting everybody!
while at closed sites GATA6 initiates profound nucleosome remodeling characterized by the acquisition of central fragile nucleosomes flanked by ordered nucleosomal arrays and increased accessibility, this is fully maximized ONLY upon binding of SOX17, OCT4 and SOX2 (and to a lesser extent ESRRB)
December 12, 2025 at 11:35 PM
while at closed sites GATA6 initiates profound nucleosome remodeling characterized by the acquisition of central fragile nucleosomes flanked by ordered nucleosomal arrays and increased accessibility, this is fully maximized ONLY upon binding of SOX17, OCT4 and SOX2 (and to a lesser extent ESRRB)
GATA6 leads to rapid downregulation of the pluripotency network and to the upregulation of PrE genes.
It acts opportunistically and transiently at regions fragilized by pluripotency TFs, even with poor GATAA motifs, and as a pioneer at closed sites with good motifs 🤯
It acts opportunistically and transiently at regions fragilized by pluripotency TFs, even with poor GATAA motifs, and as a pioneer at closed sites with good motifs 🤯
December 12, 2025 at 11:35 PM
GATA6 leads to rapid downregulation of the pluripotency network and to the upregulation of PrE genes.
It acts opportunistically and transiently at regions fragilized by pluripotency TFs, even with poor GATAA motifs, and as a pioneer at closed sites with good motifs 🤯
It acts opportunistically and transiently at regions fragilized by pluripotency TFs, even with poor GATAA motifs, and as a pioneer at closed sites with good motifs 🤯
I let you go through this massive paper, but we tried to summarize all findings in the cartoon ⬇️⬇️⬇️
December 12, 2025 at 11:34 PM
I let you go through this massive paper, but we tried to summarize all findings in the cartoon ⬇️⬇️⬇️
Massive work, ~250 libraries and 60 billion reads (it took @pablonavarrolab.bsky.social and me >6 months to analyze all the data and try to make sense of it)
a man sits at a desk in front of a computer and the word work is visible
ALT: a man sits at a desk in front of a computer and the word work is visible
media.tenor.com
December 12, 2025 at 11:32 PM
Massive work, ~250 libraries and 60 billion reads (it took @pablonavarrolab.bsky.social and me >6 months to analyze all the data and try to make sense of it)
Warning: thread heavy on epigenomics. I profiled TFs (GATA6, SOX17, OCT4, SOX2 & ESRRB) accessibility (ATAC-seq), nucleosomes (MNase-seq), and gene expression (RNA-seq) over days of GATA6 induction, combined with Oct4 kd or AID-mediated degradation.
December 12, 2025 at 11:31 PM
Warning: thread heavy on epigenomics. I profiled TFs (GATA6, SOX17, OCT4, SOX2 & ESRRB) accessibility (ATAC-seq), nucleosomes (MNase-seq), and gene expression (RNA-seq) over days of GATA6 induction, combined with Oct4 kd or AID-mediated degradation.
The 🌟 of the paper is GATA6, key to make primitive endoderm in blastocysts and from ES cells
GATA6 belongs to a family with two of the first ever recognized pioneer TFs as shown by:
1/ G.Felsenfeld (doi.org/10.1006/jmbi...)
2/ K.Zaret (doi.org/10.1016/s109...
GATA6 belongs to a family with two of the first ever recognized pioneer TFs as shown by:
1/ G.Felsenfeld (doi.org/10.1006/jmbi...)
2/ K.Zaret (doi.org/10.1016/s109...
December 12, 2025 at 11:30 PM
The 🌟 of the paper is GATA6, key to make primitive endoderm in blastocysts and from ES cells
GATA6 belongs to a family with two of the first ever recognized pioneer TFs as shown by:
1/ G.Felsenfeld (doi.org/10.1006/jmbi...)
2/ K.Zaret (doi.org/10.1016/s109...
GATA6 belongs to a family with two of the first ever recognized pioneer TFs as shown by:
1/ G.Felsenfeld (doi.org/10.1006/jmbi...)
2/ K.Zaret (doi.org/10.1016/s109...
I (further) showed that pioneer TFs conspire among themselves to:
1/ remodel nucleosomes
2/ rewire gene regulatory networks
3/ elicit cell differentiation
One is good, but many is better! (Inspired by @pablonavarrolab.bsky.social original twittorial)
1/ remodel nucleosomes
2/ rewire gene regulatory networks
3/ elicit cell differentiation
One is good, but many is better! (Inspired by @pablonavarrolab.bsky.social original twittorial)
December 12, 2025 at 11:30 PM
I (further) showed that pioneer TFs conspire among themselves to:
1/ remodel nucleosomes
2/ rewire gene regulatory networks
3/ elicit cell differentiation
One is good, but many is better! (Inspired by @pablonavarrolab.bsky.social original twittorial)
1/ remodel nucleosomes
2/ rewire gene regulatory networks
3/ elicit cell differentiation
One is good, but many is better! (Inspired by @pablonavarrolab.bsky.social original twittorial)
Il a aussi été l'avocat de mila il me semble, à croire que tous les racismes / antisémitismes ne le dérangent pas...
November 29, 2025 at 8:10 AM
Il a aussi été l'avocat de mila il me semble, à croire que tous les racismes / antisémitismes ne le dérangent pas...
polémique initiée par damien rieu qui n'est pas du tout facho ni mascu d'ailleurs. Il manque aussi le début de la vidéo dans lequel on voit les actrices (brillantes) de la comédie musicale, décidément à part la calomnie et le mensonge qu'avez vous comme argument?
November 24, 2025 at 3:00 PM
polémique initiée par damien rieu qui n'est pas du tout facho ni mascu d'ailleurs. Il manque aussi le début de la vidéo dans lequel on voit les actrices (brillantes) de la comédie musicale, décidément à part la calomnie et le mensonge qu'avez vous comme argument?
C'est pas plutôt le camp des racistes et autres droitards extrêmes avec qui tu t'affiches le masculinisme et le racisme? L'inversion accusatoire aussi au passage 🤡🤡🤡🤡
November 24, 2025 at 2:50 PM
C'est pas plutôt le camp des racistes et autres droitards extrêmes avec qui tu t'affiches le masculinisme et le racisme? L'inversion accusatoire aussi au passage 🤡🤡🤡🤡
Elle est toujours employée par le CNRS et ne manque pas de le rappeler (comme argument d'autorité). Ce n'est pas mon domaine mais il me semble que sa production scientifique récente est faible et de plus très critiquée
November 14, 2025 at 7:10 PM
Elle est toujours employée par le CNRS et ne manque pas de le rappeler (comme argument d'autorité). Ce n'est pas mon domaine mais il me semble que sa production scientifique récente est faible et de plus très critiquée