Ron Beavis ❌
@ronbeavis.mastodon.social.ap.brid.gy
Doing science for money since 1981.
Putting it online since 1995.
Just say no to cancer research (lesson learned).
QA >> QC
I enjoy doing things I am not very good […]
🌉 bridged from ⁂ https://mastodon.social/@RonBeavis, follow @ap.brid.gy to interact
Putting it online since 1995.
Just say no to cancer research (lesson learned).
QA >> QC
I enjoy doing things I am not very good […]
🌉 bridged from ⁂ https://mastodon.social/@RonBeavis, follow @ap.brid.gy to interact
The polar vortex has re-established itself into a nearly circular pattern. (winds @ 10 hPa)
December 7, 2025 at 1:31 PM
The polar vortex has re-established itself into a nearly circular pattern. (winds @ 10 hPa)
I would have hoped that at least a few "smart" people might have read this before it was published. Reads like an essay about a piece of PK Dick/Harry Turteldove alternate history fiction.
https://www.whitehouse.gov/wp-content/uploads/2025/12/2025-National-Security-Strategy.pdf
https://www.whitehouse.gov/wp-content/uploads/2025/12/2025-National-Security-Strategy.pdf
December 6, 2025 at 10:48 PM
I would have hoped that at least a few "smart" people might have read this before it was published. Reads like an essay about a piece of PK Dick/Harry Turteldove alternate history fiction.
https://www.whitehouse.gov/wp-content/uploads/2025/12/2025-National-Security-Strategy.pdf
https://www.whitehouse.gov/wp-content/uploads/2025/12/2025-National-Security-Strategy.pdf
I would say this about pretty much any field: it is just a little more ironic when discussing applied math.
https://www.theguardian.com/technology/2025/dec/06/ai-research-papers
https://www.theguardian.com/technology/2025/dec/06/ai-research-papers
December 6, 2025 at 7:08 PM
I would say this about pretty much any field: it is just a little more ironic when discussing applied math.
https://www.theguardian.com/technology/2025/dec/06/ai-research-papers
https://www.theguardian.com/technology/2025/dec/06/ai-research-papers
If any gene needs a hard reset on its literature, it is MUC1. It is a confusing mess for a bunch of reasons.
December 6, 2025 at 3:19 PM
If any gene needs a hard reset on its literature, it is MUC1. It is a confusing mess for a bunch of reasons.
I kind of like taking servers completely apart, cleaning them & putting them back together again. It is a reminder of their impermanence & removes any glamour from their operation.
December 4, 2025 at 8:34 PM
I kind of like taking servers completely apart, cleaning them & putting them back together again. It is a reminder of their impermanence & removes any glamour from their operation.
This week's example of completely unnecessary pharmaceutical research coupled with people who really shouldn't have a cat ...
https://abcnews.go.com/GMA/Wellness/weight-loss-drugs-cats-company-launches-clinical-trial/story?id=128054236
https://abcnews.go.com/GMA/Wellness/weight-loss-drugs-cats-company-launches-clinical-trial/story?id=128054236
Weight loss drugs for cats? Company launches clinical trial of GLP-1 implants in cats
The new implants will be for weight loss in cats and dogs
abcnews.go.com
December 4, 2025 at 2:58 PM
This week's example of completely unnecessary pharmaceutical research coupled with people who really shouldn't have a cat ...
https://abcnews.go.com/GMA/Wellness/weight-loss-drugs-cats-company-launches-clinical-trial/story?id=128054236
https://abcnews.go.com/GMA/Wellness/weight-loss-drugs-cats-company-launches-clinical-trial/story?id=128054236
Pretty much a case study of how busted US capitalism is at the moment.
https://www.forbes.com/sites/zoyahasan/2025/12/03/this-25-year-old-founder-wants-to-kick-chinese-drones-out-of-american-skies
https://www.forbes.com/sites/zoyahasan/2025/12/03/this-25-year-old-founder-wants-to-kick-chinese-drones-out-of-american-skies
December 4, 2025 at 2:12 PM
Pretty much a case study of how busted US capitalism is at the moment.
https://www.forbes.com/sites/zoyahasan/2025/12/03/this-25-year-old-founder-wants-to-kick-chinese-drones-out-of-american-skies
https://www.forbes.com/sites/zoyahasan/2025/12/03/this-25-year-old-founder-wants-to-kick-chinese-drones-out-of-american-skies
Name a type of research that is unlikely to be featured at Thermo users meetings.
https://proteomicsnews.blogspot.com/2025/12/opt-tmt-scale-down-everything-so-you.html
https://proteomicsnews.blogspot.com/2025/12/opt-tmt-scale-down-everything-so-you.html
opt-TMT -scale down everything so you aren't wasting so much reagent!
There is another optTMT, but that one doesn't have a dash and it's for designing smart multi-batch mutiplexed experiments. You can read about that one here.
This new one is about how a lot of TMT labs are labeling 400 pounds of peptide (181 kg) and then injecting 200 micrograms per injection on their Orbitraps and 1000 micrograms on their Astrals.
If you wanted to just label 10x more peptide than you'd possibly use instead of 10 million times more peptide, how would you do it? That's what the dash is for!
While this might seem just a little silly since there are protocols out there that have been replicated dozens of times for labeling single human cells, they are actually a lot more convenient than you'd think. We know how much reagent in our lab to use for 1 cell or 25 cells and it's a drag when we have to break out the peptide quan kits and borrow someone's plate reader. This study gives you that in-between concentration fully optimized.
proteomicsnews.blogspot.com
December 2, 2025 at 3:46 PM
Name a type of research that is unlikely to be featured at Thermo users meetings.
https://proteomicsnews.blogspot.com/2025/12/opt-tmt-scale-down-everything-so-you.html
https://proteomicsnews.blogspot.com/2025/12/opt-tmt-scale-down-everything-so-you.html
The polar vortex (winds @ 10 hPa) is beginning to close up & detach its circulation from the high pressure system that is sliding south down the Pacific coast.
December 2, 2025 at 1:14 PM
The polar vortex (winds @ 10 hPa) is beginning to close up & detach its circulation from the high pressure system that is sliding south down the Pacific coast.
Does anybody know if an N-degron mechanism has been discovered for proteins with unblocked N-terminal alanines? I can't seem to find one, but finding anything in "the literature" has become a hit or miss proposition.
December 1, 2025 at 5:13 PM
Does anybody know if an N-degron mechanism has been discovered for proteins with unblocked N-terminal alanines? I can't seem to find one, but finding anything in "the literature" has become a hit or miss proposition.
Nuclear receptor subfamily 3 group C member 1 (NR3C1:p) has 2 rigid features: a C-terminal ligand binding (in this case glucocorticoid) domain & a smaller zinc finger domain. The rest is a large dynamic/active region divvied up by a grid of PUZZLE domains (6 pS & 1 pT). #proteomics
December 1, 2025 at 1:03 PM
Nuclear receptor subfamily 3 group C member 1 (NR3C1:p) has 2 rigid features: a C-terminal ligand binding (in this case glucocorticoid) domain & a smaller zinc finger domain. The rest is a large dynamic/active region divvied up by a grid of PUZZLE domains (6 pS & 1 pT). #proteomics
A new feature I'm sure everyone will appreciate.
https://www.forbes.com/sites/zakdoffman/2025/11/30/google-starts-sharing-all-your-text-messages-with-your-employer/?ss=cybersecurity
https://www.forbes.com/sites/zakdoffman/2025/11/30/google-starts-sharing-all-your-text-messages-with-your-employer/?ss=cybersecurity
Google Starts Sharing All Your Text Messages With Your Employer
Warning — what happens on your Android phone, doesn’t stay on your Android phone. Not if it's a work phone.
www.forbes.com
November 30, 2025 at 7:52 PM
A new feature I'm sure everyone will appreciate.
https://www.forbes.com/sites/zakdoffman/2025/11/30/google-starts-sharing-all-your-text-messages-with-your-employer/?ss=cybersecurity
https://www.forbes.com/sites/zakdoffman/2025/11/30/google-starts-sharing-all-your-text-messages-with-your-employer/?ss=cybersecurity
"N-terminal methionine excision from newly synthesized proteins, catalyzed cotranslationally by methionine aminopeptidases (METAPs), is an essential and universally conserved process that plays a key role in cell homeostasis and protein biogenesis." (Science, 22 Jun 2023, 380:6651, 1238-1243).
November 30, 2025 at 6:09 PM
"N-terminal methionine excision from newly synthesized proteins, catalyzed cotranslationally by methionine aminopeptidases (METAPs), is an essential and universally conserved process that plays a key role in cell homeostasis and protein biogenesis." (Science, 22 Jun 2023, 380:6651, 1238-1243).
The combination of a low pressure system centred in Michigan, a high off-shore of Maine are pumping up some wet air from the Atlantic & the Gulf of the Mad King is generating a storm in the upper Midwestern states & Southern Ontario.
November 30, 2025 at 3:14 PM
The combination of a low pressure system centred in Michigan, a high off-shore of Maine are pumping up some wet air from the Atlantic & the Gulf of the Mad King is generating a storm in the upper Midwestern states & Southern Ontario.
Nuclear receptor interacting protein 1 (NRIP1:p) is believed to interact with the ligand binding domains of nuclear receptors, modulating their transcription factor activity. It does so without any 2°/3° structure, but uses a grid of pS PUZZLE domain controls. #proteomics
November 30, 2025 at 1:19 PM
Nuclear receptor interacting protein 1 (NRIP1:p) is believed to interact with the ligand binding domains of nuclear receptors, modulating their transcription factor activity. It does so without any 2°/3° structure, but uses a grid of pS PUZZLE domain controls. #proteomics
What happens when a cat sits on a laptop keyboard.
November 29, 2025 at 4:39 PM
What happens when a cat sits on a laptop keyboard.
Have any #proteomics information analysis systems been developed that try to explain experimental results in terms of transcription factors & E3 ligases rather than metabolic/functional pathways?
November 29, 2025 at 3:59 PM
Have any #proteomics information analysis systems been developed that try to explain experimental results in terms of transcription factors & E3 ligases rather than metabolic/functional pathways?
[Contains proteomics information involving splice variants]
Human ligand dependent nuclear receptor corepressor (LCOR:p) has 2 easily observed splice variants, 433 aa & 1557 aa, in which the 3rd exon gets swapped out for a very different sequence. The […]
[Original post on mastodon.social]
Human ligand dependent nuclear receptor corepressor (LCOR:p) has 2 easily observed splice variants, 433 aa & 1557 aa, in which the 3rd exon gets swapped out for a very different sequence. The […]
[Original post on mastodon.social]
November 29, 2025 at 12:48 PM
[Contains proteomics information involving splice variants]
Human ligand dependent nuclear receptor corepressor (LCOR:p) has 2 easily observed splice variants, 433 aa & 1557 aa, in which the 3rd exon gets swapped out for a very different sequence. The […]
[Original post on mastodon.social]
Human ligand dependent nuclear receptor corepressor (LCOR:p) has 2 easily observed splice variants, 433 aa & 1557 aa, in which the 3rd exon gets swapped out for a very different sequence. The […]
[Original post on mastodon.social]
Ring finger protein 187 (RNF187:p) initiates translation at a "CGT" triplet (Leu), rather than the much more commonly used "AGT" (Met). Interestingly (for me) is the fact that the N-terminal L is co-translationally removed & N-acetyl is added to alanine exposed by that operation. #proteomics
November 28, 2025 at 8:07 PM
Ring finger protein 187 (RNF187:p) initiates translation at a "CGT" triplet (Leu), rather than the much more commonly used "AGT" (Met). Interestingly (for me) is the fact that the N-terminal L is co-translationally removed & N-acetyl is added to alanine exposed by that operation. #proteomics
I have heard several podcasts this week in which a Canadian commentator hosts an American commentator regarding the "51st state" meme. Unfortunately in the ones I have heard so far, the Canadian has never lived in the US & the American has never lived in Canada, so nothing of value can be discussed.
November 28, 2025 at 3:37 PM
I have heard several podcasts this week in which a Canadian commentator hosts an American commentator regarding the "51st state" meme. Unfortunately in the ones I have heard so far, the Canadian has never lived in the US & the American has never lived in Canada, so nothing of value can be discussed.
When Gemini is asked: "What is the best brand of mass spectrometer to use in proteomics experiments?", after a lot of slop it answers:
November 28, 2025 at 1:48 PM
When Gemini is asked: "What is the best brand of mass spectrometer to use in proteomics experiments?", after a lot of slop it answers:
The polar vortex is continuing to open up over the North Atlantic, with the counter-rotating system now centred on the northern shore of the Yukon. (winds @ 10 hPa)
November 28, 2025 at 12:53 PM
The polar vortex is continuing to open up over the North Atlantic, with the counter-rotating system now centred on the northern shore of the Yukon. (winds @ 10 hPa)
Like NCOR1:p, nuclear receptor corepressor 2 (NCOR2:p) has ~5% of its sequence tied up in rigid structure (2× homeobox domains of unknown DNA specificity). The remaining 95% of the protein is chopped up into >10 evolutionarily conserved dynamic PUZZLE domains that do the work here. #proteomics
November 28, 2025 at 12:47 PM
Like NCOR1:p, nuclear receptor corepressor 2 (NCOR2:p) has ~5% of its sequence tied up in rigid structure (2× homeobox domains of unknown DNA specificity). The remaining 95% of the protein is chopped up into >10 evolutionarily conserved dynamic PUZZLE domains that do the work here. #proteomics