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Home - SEVA plasmids - Standard European Vector Architecture

sevaplasmids.bsky.social @sevaplasmids.bsky.social · 2d

⏳ Stop wasting hours searching for the perfect plasmid for your experiment.

👉 Discover @SEVAplasmids — a community-driven, non-profit collection of standardised plasmids.

🔬 Open, reliable, and ready for your research.

Check it out here 👇
seva-plasmids.com

Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

Scaling laws of bacterial and archaeal plasmids - Nature Communications

sevaplasmids.bsky.social @sevaplasmids.bsky.social · 5d

📰 A computational analysis reveals 3️⃣ scaling laws of plasmids. Longer plasmids have:

1️⃣fewer copies
2️⃣more protein-coding
3️⃣more metabolic genes

And with gradual adoption of chromosome-like features as they become longer. For more details 👉 doi.org/10.1038/s414...

The capacity of a plasmid to express genes is constrained by parameters such as its length and copy number. Here, Maddamsetti et al. present a computational method that enables rapid and accurate…

Creating a Genetic Toolbox for the Carbon-Fixing, Nitrogen-Fixing and Dehalogenating Bacterium Xanthobacter autotrophicus

sevaplasmids.bsky.social @sevaplasmids.bsky.social · 7d

🧬🔧X. autotrophicus, despite its metabolic flexibility and biosynthetic potential, has few genetic tools available. Researchers from Northeastern University introduce a new toolbox for this microbe, enabling optimisation of processes like bioremediation👉https://doi.org/10.1021/acssynbio.4c00455

Xanthobacter autotrophicus is a metabolically flexible microorganism with two key features: (1) The organism has adapted to grow on a wide variety of carbon sources including CO2, methanol, formate,…

A framework for complex signal processing via synthetic biological operational amplifiers - Nature Communications

sevaplasmids.bsky.social @sevaplasmids.bsky.social · 9d

📢 A study in Nature Communications presents a new framework that integrates scalable orthogonal amplifiers into standardised biological processes, increasing precision, adaptability, and signal-to-noise ratio in complex genetic circuits. Read the full study here👉 doi.org/10.1038/s414...

Engineering genetic circuits to control gene expression in response to complex signal inputs remains a challenge due to limited precision. This study introduces operational amplifiers that decode…

CONTACT - SEVA plasmids - Standard European Vector Architecture

sevaplasmids.bsky.social @sevaplasmids.bsky.social · 12d

With #SEVAplasmids you’re just 2 steps away from your ideal plasmid!

1️⃣ Design your plasmid
2️⃣ Fill in the super simple order form

That’s it —your customised plasmids will be shipped straight to your lab 🚀 at no cost
Start now👉 seva-plasmids.com/contact/

Order plasmids If you wish to order SEVA plasmids, please fill in the form below. We offer this service pro bono so it is free of charge. Large orders ( > 15 plasmids) may experience shipment delays…

Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling - Microbial Cell Factories

sevaplasmids.bsky.social @sevaplasmids.bsky.social · 14d

🧑🤝🧑CRISPR/Cpf1 × pSEVA: a powerhouse combo in #SynBio! German researchers applied this strategy in Photorhabdus & Xenorhabdus, boosting production of high-value compounds like safracin B, a precursor to the anti-cancer drug ET-743. Read here👉 doi.org/10.1186/s129...

Background Bacteria of the genus Photorhabdus and Xenorhabdus are motile, Gram-negative bacteria that live in symbiosis with entomopathogenic nematodes. Due to their complex life cycle, they produce…

Tom Ellis Lab - Publications, Resources and PhD Thesis from the Synthetic Genomics and Synthetic Biology Research Group at Imperial College London. A bioengineering and biotechnology research group…

sevaplasmids.bsky.social @sevaplasmids.bsky.social · 16d

Meet one of our partners: @proftomellis.bsky.social's Lab, advancing genetically modified living materials. They use bacteria and yeast to create innovative biomaterials and were key in expanding SEVA from bacteria to eukaryotes. Find out more 👉https://www.tomellislab.com/our-research

RESEARCH | Tom Ellis Lab

www.tomellislab.com

Boosting energy metabolism and biosynthesis in diverse organisms by a common bacterial salvage lipoylation protein - Nature Communications

sevaplasmids.bsky.social @sevaplasmids.bsky.social · 20d

#SynBio never ceases to amaze: a new study has succeeded in increasing energy metabolism in mammalian cells, algae, and fungi through the expression of E. coli LplA, a lipoylation protein. The result? Read here 👉 doi.org/10.1038/s414...

Lipoylated proteins are essential for energy metabolism of both prokaryotes and eukaryotes. Here, the authors showed that the E. coli lipoate-protein ligase A (LplA) can boost the lipoylation of key…

Home - SEVA plasmids - Standard European Vector Architecture

sevaplasmids.bsky.social @sevaplasmids.bsky.social · 22d

Why choose between standardisation and adaptability when you can have both? With #SEVA, you can mix and match:

✅ Antibiotic resistance marker
✅ Origins of replication
✅ Cargo modules for your experiment

Build your reproducible #SynBio project here👉 seva-plasmids.com

Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

Tom Ellis Lab - Publications, Resources and PhD Thesis from the Synthetic Genomics and Synthetic Biology Research Group at Imperial College London. A bioengineering and biotechnology research group…

sevaplasmids.bsky.social @sevaplasmids.bsky.social · 26d

Meet one of our partners: @proftomellis.bsky.social's Lab, advancing genetically modified living materials. They use bacteria and yeast to create innovative biomaterials and were key in expanding SEVA from bacteria to eukaryotes. Find out more 👉https://www.tomellislab.com/our-research

RESEARCH | Tom Ellis Lab

www.tomellislab.com

sevaplasmids.bsky.social @sevaplasmids.bsky.social · 28d

🚨 News for marine biosafety: Chinese researchers engineered a Vibrio natriegens strain, capable of degrading five water organic contaminants: biphenyl, phenol, naphthalene, dibenzofuran, and toluene. Find out how they did it 👉https://doi.org/10.1038/s41586-025-08947-7

Bioremediation of complex organic pollutants by engineered Vibrio natriegens - Nature

A synthetic biology approach was used to engineer Vibrio natriegens into a strain capable of bioremediating complex organic pollutants in saline wastewater and soils, thereby addressing notable…

A single-plasmid-based, easily curable CRISPR/Cas9 system for rapid, iterative genome editing in Pseudomonas putida KT2440 - Microbial Cell Factories

sevaplasmids.bsky.social @sevaplasmids.bsky.social · Sep 8

⏳Looking for fast genome editing systems? Researchers from @uwaterloo.ca & Chinese Academy of Sciences created an all-in-one plasmid CRISPR/Cas9 system for Pseudomonas putida KT2440, enabling a genome edit in less than 1.5 days. Explore the work 👉 doi.org/10.1186/s129...

Background Pseudomonas putida KT2440, a non-pathogenic soil bacterium, is a key platform strain in synthetic biology and industrial applications due to its robustness and metabolic versatility.…

FIND YOUR PLASMID - SEVA plasmids - Standard European Vector Architecture

sevaplasmids.bsky.social @sevaplasmids.bsky.social · Sep 4

3️⃣ 2️⃣ 1️⃣... Not a countdown—it’s the standard code of one of our plasmids!

3️⃣: Cm resistance
2️⃣: RK2 origin of replication
1️⃣: MCS-default cargo

Put them together, and voilà: pSEVA321 plasmid. Simple as that!

Find more standardised plasmids here👉 seva-plasmids.com/find-your-pl...

Canonical SEVA plasmids that use Golden Gate assembly to build complex genetic circuits. Find your canonical plasmid In the dropdown menu below, you can select a combination of choice for your…

Designing pathways for bioproducing complex chemicals by combining tools for pathway extraction and ranking - Nature Communications

sevaplasmids.bsky.social @sevaplasmids.bsky.social · Sep 1

Creating complete biosynthetic pathways from scratch can be a nightmare. SubNetX is a tool that helps you design and integrate them into whole-cell models, optimising the production of complex biochemical compounds. Discover how it works 👉 doi.org/10.1038/s414...

Synthesis of complex molecules requires reactions from multiple pathways operating in balanced subnetworks. Here, the authors report a computational algorithm that combines the strength of…

Modular plasmid design for autonomous multi-protein expression in Escherichia coli - Journal of Biological Engineering

sevaplasmids.bsky.social @sevaplasmids.bsky.social · Aug 29

Could lignin waste inspire the biomanufacturing of the future?
An international team evolved Acinetobacter baylyi to consume multiple lignin-derived aromatics simultaneously, paving the way for circular bio-based production. Read the article:
👉 doi.org/10.1093/jamb...

sevaplasmids.bsky.social @sevaplasmids.bsky.social · Aug 27

SynBio still needs tools enabling independent & precise co-expression of multiple genes. Researchers at Wrocław University presented a new plasmid set with 4 regulatory systems applied in E.coli, enabling independent induction of up to 3 genes in a single vector. Learn more 👉 doi.org/10.1186/s130...

Background Molecular and synthetic biology tools enable the design of new-to-nature biological systems, including genetically engineered microorganisms, recombinant proteins, and novel metabolic…

Home - SEVA plasmids - Standard European Vector Architecture

sevaplasmids.bsky.social @sevaplasmids.bsky.social · Aug 25

🧬 Simplify your genetic engineering! #SEVAplasmids offers a growing collection of modular, “plug-and-play” plasmids designed to accelerate #SynBio workflows. Choose, combine, and customise with confidence thanks to our clear standards. 👉 seva-plasmids.com

Standard European Vector Architecture 4.0 Welcome to the CNB-hosted database and material repository of standard and modular plasmid vectors for (de/re) constructing complex bacterial phenotypes WHAT…

sevaplasmids.bsky.social @sevaplasmids.bsky.social · Aug 22

New #SynBio tool! Juan Nogales' lab presents GENIO, a platform for precise genome integration in Pseudomonas putida inspired by #SEVAplasmids’ modular design. It helps you optimise genetic circuit performance for complex synbio design. Learn more: doi.org/10.1111/1751...

sevaplasmids.bsky.social @sevaplasmids.bsky.social · Aug 20

🎯 Looking for a plasmid with specific features? Use our #SEVAplasmids selector to filter by origin, antibiotic resistance, or cargo. 👉 Find your right tool in seconds: seva-plasmids.com/select-a-pla...

Engineered microbial platform confers resistance against heavy metals via phosphomelanin biosynthesis - Nature Communications

sevaplasmids.bsky.social @sevaplasmids.bsky.social · Aug 18

🔬 A new microbial platform shows enhanced resistance to heavy metals thanks to phosphomelanin biosynthesis—plus it degrades metallised plastic waste! This could offer promising options for environmental cleanup. Read more 👉 doi.org/10.1038/s414...

Heavy metals and plastics are two major environmental pollutants. Here, the authors engineer microbial platforms capable of endogenously biosynthesizing phosphomelanin for simultaneously heavy metals…

BACKBONE MODULES & NOMENCLATURE - SEVA plasmids - Standard European Vector Architecture

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sevaplasmids.bsky.social @sevaplasmids.bsky.social · Aug 13

🧬 Understanding a plasmid’s structure at a glance? Our interactive map makes it possible. Explore SEVA’s modular architecture and its standardised nomenclature.🔍 ORI, markers, cargo… all just one click away 👉 seva-plasmids.com/backbone-mod...

Plasmid Backbone The colored arrows above the cargo and AbR modules represent the directionality of the transcription flow of that element SEVA Plasmid Nomenclature The figure sketches the expanded…

COLLABORATORS & RESOURCES - SEVA plasmids - Standard European Vector Architecture

sevaplasmids.bsky.social @sevaplasmids.bsky.social · Aug 8

🤝 Meet Lars M. Blank, partner of #SEVAplasmids community. His laboratory works to advance the bioeconomy by exploring microbial metabolism to transform CO₂, biomass, and plastic waste into valuable chemicals. Meet other collaborators: seva-plasmids.com/the-seva-com...

The SEVA commonwealth Laboratory of Dr. Sven Panke. Bioprocess laboratory. ETH. Switzerland. Laboratory of Dr. Nediljko Budisa.

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sevaplasmids.bsky.social @sevaplasmids.bsky.social · Aug 6

🌱Researchers at the University of Minnesota engineered a strain of Azotobacter vinelandii to release nitrogen as urea. According to their paper, they achieved a 43× higher yield than before. A promising advance for sustainable agriculture and next-gen biofertilisers 👉 doi.org/10.1111/1751...

sevaplasmids.bsky.social @sevaplasmids.bsky.social · Aug 5

🌞 Summer break is here!
The activity of @sevaplasmids.bsky.social is paused for the holidays, so plasmid shipments are on hold until September.
🧬 You can still place your orders — we'll get back to you when we return.
See you in September!