Using transcript-aware knockdown we found that endogenous ncISG15, and not ISG15, is antiviral against influenza A virus through polymerase restriction. But life finds a way, and influenza A NS1 antagonizes ncISG15. ncISG15 seems widely conserved- we’re excited how this finding shapes the field! 4/4

Seminal work by Lenschow, Krug, and Bogunovic found that ISG15 is antiviral against influenza A and B virus in mice, but in humans ISG15 antagonizes influenza B virus and less so A. This is in part explained by ISG15’s ability to dampen inflammation 3/4

Non-canonical ISG15 (ncISG15) encodes an 8 aa N-terminal truncation. Like ISG15 it post-translationally modifies proteins and is secreted, but ISG15 is 100X more abundant (explaining its lack of ID). Things got spicy when we tested ncISG15’s function during influenza A viral infection 2/4

Also looking forward to what you write!

Hiiiii - final version now available: journals.asm.org/doi/10.1128/.... The work by Himadri, Ariel, and Josh shows that there's no need for glasses to see infected cells with these split-GFP viruses.

Ah I’ll look into GoodNotes. The PDF reader needs to be A+, that’s the most important feature. Paperpile lets you read PDFs in dark mode 🤯 so it might be hard to beat.