Lankey Kong from Donkey Kong 64
Tetra from The Legend of Zelda: The Wind Waker
Eslo from The Legend of Zelda: The Minish Cap
Roulette Boy, Judgement Boy, Death, Poor Inductor and Public Phone from Gregory Horror Show

Atsuko, Atsuko (mouse form), Sucy, Amanda and Ursula from Little Witch Academia
Waluigi

Queen from Deltarune

One I just now remembered the name of:
Marsupilami from Marsupilami

Forgot one:
Maestro from Globi and the Stolen Shadows

Oggy and the Cockroaches from Oggy and the Cockroaches
Felix der Hase from Briefe von Felix
Werner from Werner
Käpt'n Blaubär from Käpt'n Blaubärs Seemannsgarn
Asterix and Obelix from Asterix
Bruno from Ottos Ottifanten
Tom from Tom und das Erdbeermarmeladebrot mit Honig

Brigel der Busch and Chili das Schaf from Bernd das Brot
Globi from the Globi books
The Guide and The Dryad from Terraria
Rolli and Rita from Hallo aus Berlin
Toss Boys from Rhytm Heaven
Stitch from Lilo & Stitch
Tom Turbo from Tom Turbo
Helmi from Helmi

Blue from Foster's Home for Imaginary Friends
Wander and Yonder from Wander Over Yonder
Dan and Dan* *Imposter from Dan Vs.
Peter Griffin from Family Guy
Charles and Ellie from Henry Stickmin
Haselhörnchen and Jammerlappen from Haselhörnchen - Hier knallt die Ente

Dave Strider, Jade Harley, Dr. Scratch and Teresi from Homestuck
Twilight Sparkle, Maud Pie, Discord, Thorax (changeling form), Boulder and Tom from MLP:FiM
Izzy Moonbow from MLP gen 5
Stanley Spadowski from UHF
Harvey The Wonder Hamster from The Weird Al Show
Garnet from Steven Universe

ur welcome!!

Because you are gifting a lot of gifts this merry holiday :)

bonus

So does chiitan think

the blondie with the cool shades and the record shirt

they do be

Enough! My ship sails in the morning.

Someone should make a mod where the player with the second Famicom can pause the games by screaming into the microphone