stevebrohawn.bsky.social
Steve Brohawn
@stevebrohawn.bsky.social
390 followers 210 following 6 posts
Scientist at UC Berkeley in Molecular and Cell Biology and Neuroscience. Ion channels, electrophysiology, optogenetics, cryo-EM, cryo-ET. https://www.brohawnlab.org/ https://scholar.google.com/citations?user=AFDQBS0AAAAJ&hl=en
www.brohawnlab.org
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stevebrohawn.bsky.social
Steve Brohawn @stevebrohawn.bsky.social · Jan 16
Please spread the word and apply!
jimhurley.bsky.social
Jim Hurley @jimhurley.bsky.social · Jan 15
Scientific Director, Cal-Cryo. We are looking for an outstanding microscopist to run our cryo-EM facility (two Krioses and an Arctica), increase training, engagement, and collaboration on campus, expand our user base, and join our community of cutting edge cryo-EM researchers. tinyurl.com/ykasna3p
Scientific Director, Cal-Cryo@QB3, UC Berkeley’s Cryo-EM Facility
University of California, Berkeley is hiring. Apply now!
tinyurl.com
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stevebrohawn.bsky.social
Steve Brohawn @stevebrohawn.bsky.social · Dec 10
Preprint is up! www.biorxiv.org/content/10.1... Trevor Docter + Ben Sorum found cannabinoids inhibit mechanosensitive K+ channels, including TRAAK mutants that cause epilepsy and FHEIG in humans. #ephys and #cryoem shows CBD competes with lipids to bind inside the channel cavity and block the pore.
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Reposted by Steve Brohawn
mackthompson88.bsky.social
Mack Thompson @mackthompson88.bsky.social · Dec 3
So happy to finally share my final paper from my PhD! By collecting cryo-EM datasets at sub-saturating concentrations of ACh, we were able to determine a mono-liganded state of the muscle-type nAChR. This state has a fully activated alpha subunit where ACh binds…

www.biorxiv.org/content/10.1...
Asynchronous subunit transitions precede acetylcholine receptor activation
Rapid communication at synapses is facilitated by postsynaptic receptors, which convert a chemical signal into an electrical response. In the case of ligand-gated ion channels, agonist binding trigger...
www.biorxiv.org
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stevebrohawn.bsky.social
Steve Brohawn @stevebrohawn.bsky.social · Dec 3
Congrats!
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Steve Brohawn @stevebrohawn.bsky.social · Dec 2
Thanks Anirban!
Reposted by Steve Brohawn
antonylurie.bsky.social
Antony Lurie @antonylurie.bsky.social · Nov 27
The first story from my PhD is now available on bioRxiv!: www.biorxiv.org/content/10.1...

We determined structures of a LRRC8A:D VRAC by cryo-EM and explored the role of pore lipids in their gating. Huge thanks to the whole Brohawn lab, and especially to Steve @stevebrohawn.bsky.social and David!
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stevebrohawn.bsky.social
Steve Brohawn @stevebrohawn.bsky.social · Nov 27
One more from earlier this month! www.biorxiv.org/content/10.1... We found sphingolipid binding to SARS-CoV-2 M protein controls its conformation to coordinate viral assembly and release. Great collaboration with Voth + Stahelin labs led by Kim Dolan, Mandira Dutta, and Souad Amiar #cryoem #SARSCoV2
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stevebrohawn.bsky.social
Steve Brohawn @stevebrohawn.bsky.social · Nov 27
Excited to share our preprint about volume regulated ion channels here! We solved structures of heteromeric LRRC8A:D channels and show how they are gated closed by pore-blocking lipids. Led by @antonylurie.bsky.social + David Kern
#IonChannel #cryoem
www.biorxiv.org/content/10.1...
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