Susanna Teppo
@sustep.bsky.social
Bioscientist (PhD) | Molecular Biology | Biomedicine | STEM teacher
Highlighting the latest advancements in stem cell biology and regenerative medicine.
Highlighting the latest advancements in stem cell biology and regenerative medicine.
How do you address genomic instability in your hPSC cultures?
Direct links to the publications:
Krivec et al. 2024 Nature - Cell Death & Disease: doi.org/10.1038/s414...
Stavish et al. 2024 Stem Cell Reports: doi.org/10.1016/j.st...
Jacobs et al. 2016 Stem Cell Reports: doi.org/10.1016/j.st...
Direct links to the publications:
Krivec et al. 2024 Nature - Cell Death & Disease: doi.org/10.1038/s414...
Stavish et al. 2024 Stem Cell Reports: doi.org/10.1016/j.st...
Jacobs et al. 2016 Stem Cell Reports: doi.org/10.1016/j.st...
Gain of 1q confers an MDM4-driven growth advantage to undifferentiated and differentiating hESC while altering their differentiation capacity - Cell Death & Disease
Cell Death & Disease - Gain of 1q confers an MDM4-driven growth advantage to undifferentiated and differentiating hESC while altering their differentiation capacity
doi.org
November 24, 2024 at 3:43 PM
How do you address genomic instability in your hPSC cultures?
Direct links to the publications:
Krivec et al. 2024 Nature - Cell Death & Disease: doi.org/10.1038/s414...
Stavish et al. 2024 Stem Cell Reports: doi.org/10.1016/j.st...
Jacobs et al. 2016 Stem Cell Reports: doi.org/10.1016/j.st...
Direct links to the publications:
Krivec et al. 2024 Nature - Cell Death & Disease: doi.org/10.1038/s414...
Stavish et al. 2024 Stem Cell Reports: doi.org/10.1016/j.st...
Jacobs et al. 2016 Stem Cell Reports: doi.org/10.1016/j.st...
How do we best ensure genomic stability while culturing on defined and xeno-free conditions?
Jacobs et al. (2016) provided practical insights especially for high-density cultures:
• Cultures on full-length laminin-521 exhibited reduced DNA damage
• Frequent medium changes rescued genomic integrity
Jacobs et al. (2016) provided practical insights especially for high-density cultures:
• Cultures on full-length laminin-521 exhibited reduced DNA damage
• Frequent medium changes rescued genomic integrity
Gain of 1q confers an MDM4-driven growth advantage to undifferentiated and differentiating hESC while altering their differentiation capacity - Cell Death & Disease
Cell Death & Disease - Gain of 1q confers an MDM4-driven growth advantage to undifferentiated and differentiating hESC while altering their differentiation capacity
doi.org
November 24, 2024 at 3:43 PM
How do we best ensure genomic stability while culturing on defined and xeno-free conditions?
Jacobs et al. (2016) provided practical insights especially for high-density cultures:
• Cultures on full-length laminin-521 exhibited reduced DNA damage
• Frequent medium changes rescued genomic integrity
Jacobs et al. (2016) provided practical insights especially for high-density cultures:
• Cultures on full-length laminin-521 exhibited reduced DNA damage
• Frequent medium changes rescued genomic integrity
Impact of culture conditions – how to prevent instability?
Recently, Stavish et al. (2024) suggested that 1q-gained cells can only take over the culture when the cells are cultured on feeder-free conditions (such as E8/VTN).
Recently, Stavish et al. (2024) suggested that 1q-gained cells can only take over the culture when the cells are cultured on feeder-free conditions (such as E8/VTN).
Gain of 1q confers an MDM4-driven growth advantage to undifferentiated and differentiating hESC while altering their differentiation capacity - Cell Death & Disease
Cell Death & Disease - Gain of 1q confers an MDM4-driven growth advantage to undifferentiated and differentiating hESC while altering their differentiation capacity
doi.org
November 24, 2024 at 3:43 PM
Impact of culture conditions – how to prevent instability?
Recently, Stavish et al. (2024) suggested that 1q-gained cells can only take over the culture when the cells are cultured on feeder-free conditions (such as E8/VTN).
Recently, Stavish et al. (2024) suggested that 1q-gained cells can only take over the culture when the cells are cultured on feeder-free conditions (such as E8/VTN).
Elevated levels of MDM4 (in 1q) inhibit p53-mediated apoptosis, allowing damaged 1q-gained cells to persist and outcompete genetically balanced cells.
Krivec and colleagues show that 1q-gained hPSCs can also take over during differentiation and result in more immature hepatocytes and cardiomyocytes
Krivec and colleagues show that 1q-gained hPSCs can also take over during differentiation and result in more immature hepatocytes and cardiomyocytes
November 24, 2024 at 3:43 PM
Elevated levels of MDM4 (in 1q) inhibit p53-mediated apoptosis, allowing damaged 1q-gained cells to persist and outcompete genetically balanced cells.
Krivec and colleagues show that 1q-gained hPSCs can also take over during differentiation and result in more immature hepatocytes and cardiomyocytes
Krivec and colleagues show that 1q-gained hPSCs can also take over during differentiation and result in more immature hepatocytes and cardiomyocytes