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Bryan Bryson @thebrysonlab.bsky.social · 13d

Congrats to the Carpenter Lab on this exciting study!

I might now sound like a broken record, but check the abstract!!!

Let’s go type 7 secretion system substrates! #tuberculosis #tbsky

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Bryan Bryson @thebrysonlab.bsky.social · 21d

Happy to see more data pointing to an important role for type 7 secretion substrates in TB protective immunity

Cell - a Cell Press journal @cp-cell.bsky.social · 23d

Now online! Mining the CD4 antigen repertoire for next-generation tuberculosis vaccines

Mining the CD4 antigen repertoire for next-generation tuberculosis vaccines

Systematic antigen selection is an enduring challenge in tuberculosis (TB) vaccine development. An antigen screen ranks protective CD4 T cell antigens and yields a novel trivalent mRNA vaccine that protects against TB challenges in mice.

dlvr.it

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Reposted by Bryan Bryson

Journal of Experimental Medicine @jem.org · Aug 18

New video summary 🎥 from Leddy et al. @thebrysonlab.bsky.social (rupress.org/jem/article/... ) on a new workflow called PathMHC that helps find pathogen-derived antigens presented on MHCs by infected cells that could be targets for T cell priming vaccines:

Targeting infection-specific peptides in immunopeptidomics studies for vaccine target discovery

Original paper: https://rupress.org/jem/article/222/10/e20250444/278135/Targeting-infection-specific-peptides-in Video summary by Owen Leddy (Massachusetts Institute of Technology) Description: One…

youtu.be

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Bryan Bryson @thebrysonlab.bsky.social · Jul 21

Congrats to the amazing Owen Leddy for this tour de force which was inspired by reviewer comments from our previous paper and thanks to our amazing friends and collaborators for making the project a reality.

And thanks to JEM for a very pleasant and efficient review process!

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Bryan Bryson @thebrysonlab.bsky.social · Jul 21

Importantly, for Mtb, the results are consistent with our previous study (doi.org/10.7554/eLif...) where we showed that Mtb-derived peptides on MHC-I are enriched for type VII secretion system substrates underscoring our enthusiasm for including these antigens in TB vaccines.

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Bryan Bryson @thebrysonlab.bsky.social · Jul 21

Newest paper from the lab out today (doi.org/10.1084/jem....)! Here, we describe PathMHC, a novel MS-based approach to identify rare pathogen-derived peptides on MHC-I and MHC-II. We apply this to a range of pathogens Mtb, SARS-CoV-2, and Listeria.

Targeting infection-specific peptides in immunopeptidomics studies for vaccine target discovery

MS-based immunopeptidomics can facilitate vaccine antigen discovery, but self-MHC peptides greatly outnumber pathogen-derived MHC peptides, presenting a ch

doi.org

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Bryan Bryson @thebrysonlab.bsky.social · Jul 9

Anyone have experience with IV mRNA-LNP vaccination in animals? I am specifically interested in LNP formulations and what the preferred formulation for IV is.

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Bryan Bryson @thebrysonlab.bsky.social · May 5

Pictured: glass of champagne I treated myself to at DCA upon learning the lab has been tenured!

Grateful to my lab family, my extended science family, and my family for the amazing support and love offered on the journey so far.

Much more work left to do but taking this moment to say thank you!

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Bryan Bryson @thebrysonlab.bsky.social · Apr 5

Does anyone know of a CRO that produces MVA-based vaccines?

Bryan Bryson @thebrysonlab.bsky.social · Mar 28

Do any of my #tbsky friends have interests in T cell responses in people with evidence of prior Mtb infection?

If so, would you have any interest in looking at T cell responses to Mtb-derived peptides that we have detected by mass spectrometry? LMK 🙏🏽

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Reposted by Bryan Bryson

tcwarner.bsky.social @tcwarner.bsky.social · Mar 27

First post here! To inaugurate this account, I'd like to share my recent work, now published in JACS.

Intercepting a Mycobacterial Biosynthetic Pathway with Covalent Labeling | Journal of the American Chemical Society pubs.acs.org/doi/10.1021/...

Intercepting a Mycobacterial Biosynthetic Pathway with Covalent Labeling

The mycobacterial cell envelope plays both infectious and protective roles. Understanding its structure is crucial for unlocking the molecular basis underlying these functions. Studying glycans, the p...

pubs.acs.org

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Bryan Bryson @thebrysonlab.bsky.social · Jan 9

Thank you!

Bryan Bryson @thebrysonlab.bsky.social · Jan 3

Does anyone have a favorite protocol for in vitro priming of human T cells from PBMCs? #immunosky

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Bryan Bryson @thebrysonlab.bsky.social · Jan 2

So glad to see this out! I learned a lot in this project about how to think creatively about customizing our approach to using language models for immune receptors

Rohit Singh @rohitsingh8080.bsky.social · Jan 2

A bit over 3 years in the making, with first preprint out ~2 years ago, AbMAP has been a labor of love. Joint work with Chiho Im, an amazing MIT undergrad and now a fantastic Stanford grad student, and with Bonnie Berger and @thebrysonlab.bsky.social and @samsl.io and Lena Erlach and ...

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Bryan Bryson @thebrysonlab.bsky.social · Dec 12

Immunopeptidomics (impep) friends, have you ever done impep to ID peptides after soluble protein delivery to phagocytes to look at peptides generated by XP?

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Bryan Bryson @thebrysonlab.bsky.social · Dec 7

Before I write my own analysis script, does anyone in #tbsky have a tool for pathway analysis for Mtb genes?

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Bryan Bryson @thebrysonlab.bsky.social · Dec 6

A lovely day to celebrate the amazing people I work with every day

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Bryan Bryson @thebrysonlab.bsky.social · Nov 27

Yes! Can work with that!

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Bryan Bryson @thebrysonlab.bsky.social · Nov 27

Thanks! Would love any suggestions!

Bryan Bryson @thebrysonlab.bsky.social · Nov 27

Hadn’t seen this — thank you — will read!

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Bryan Bryson @thebrysonlab.bsky.social · Nov 27

Omg! This is the best news — congratulations Chuck!!!

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Bryan Bryson @thebrysonlab.bsky.social · Nov 27

Any of y’all doing expansion on bacteria specifically those that are historically difficult to permeability? If so, I have a bacterium for you ;-)

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Bryan Bryson @thebrysonlab.bsky.social · Nov 27

What is your favorite cytosolic pathogen that (a) infects macrophages and (b) is genetically tractable?

Looking for someone who’d like to team up on a small but impactful project (we would do the heavy lifting!)

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Bryan Bryson @thebrysonlab.bsky.social · Nov 25

He’s been very helpful for our project!

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Bryan Bryson @thebrysonlab.bsky.social · Nov 24

Would love to chat sometime — have been thinking about innate cells and bacteria!

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