Tsai-Hsuan Weng
@thweng.bsky.social
Structural biologist @mpibp.bsky.social | Exploring membrane transporter structures with #cryoEM ❄️🔬
Beautiful work! Congrats 🎉
May 28, 2025 at 9:30 PM
Beautiful work! Congrats 🎉
I see. So probably the first frame affects more on a clean, homogeneous particle stack? And yes there's usually ~0.2 Å improvement after polishing for the high-res structures.
November 29, 2024 at 2:26 PM
I see. So probably the first frame affects more on a clean, homogeneous particle stack? And yes there's usually ~0.2 Å improvement after polishing for the high-res structures.
Interesting. With 1 e-/Å2/fm we constantly get 2-3.5 Å res (depending on samples) without discarding the first frame using MotionCor2. In your experience, how much improvement can benifit from discarding the first frame? Or is this only for MotionCor3?
November 28, 2024 at 6:29 PM
Interesting. With 1 e-/Å2/fm we constantly get 2-3.5 Å res (depending on samples) without discarding the first frame using MotionCor2. In your experience, how much improvement can benifit from discarding the first frame? Or is this only for MotionCor3?