Excited to share that our Geneplexer and Multiplexer systems can now achieve spatial mRNA signal detection within 4.5 hrs, including hybridization, secondary amplification, and washes.

Image: A z-slice (~5 µm) of a whole 14 dpf WT zf larva stained against markers nrp1a, gad2, and neuroD.

Happy to collaborate with Ajay Mathuru's team at NUS. "Role of chrna5 in multi-substance preference and phenotypes comorbid with the development of substance dependence." Congratulations to the co-first authors, Tanisha Goel and Joshua Raine. www.biorxiv.org/content/10.1...

Glad to share the final piece of my PhD thesis. We discovered a novel Hox gene promotor that might have facilitated the evolution of phenotypic plasticity across a butterfly subfamily.

www.biorxiv.org/content/10.1...

Happy to share that our recent Bicyclus larval brain multiplex-FISH paper has been featured on the cover of Methods and Protocols.

www.mdpi.com/2409-9279/8/2

Preprint! Minos-mediated transgenesis in the pantry moth
www.biorxiv.org/content/10.1...

By @donyaniyaz.bsky.social

Sharing a user friendly online app (beta phase) to design probes for HCR3.0
tdblab.github.io/hcrprobedesi...

Raw data link: zenodo.org/records/1475...

Happy to share our latest 3D whole-mount multiplex-RNA brain scans of the larval brains of Bicyclus anynana at single-cell resolution...
We mapped multiple targets at single-cell resolution and made the raw data open source for anyone who would like to analyse it.
www.mdpi.com/2409-9279/8/...

New preprint by butterfly wizard
@lucalivraghi.bsky.social

www.biorxiv.org/content/10.1...

We mapped optix (again?!) as the switch gene of a natural polymorphism, this time controlling silver patches of a mountain butterfly. Gorgeous RNAi validation, evidence of selective sweeps, introgression

Our new manuscript describing gene multiplexing (using RAM-FISH) is finally out... We profiled multiple known and novel mRNA candidates during different developmental stages of Bicyclus and zebrafish using the easy-to-use HCR-based protocol that most labs can adapt. www.biorxiv.org/content/10.1...

Happy to be a part of the work by @tianshenbio.bsky.social published @science.org Excellent work and many more to come🎉

🎉🎉🎉

Our miRNA story is now in @science.org ! We found a microRNA, not a protein, that finally solved a long-standing evolutionary mystery of wing coloration in butterflies and moths. (1/n)
www.science.org/doi/10.1126/...