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Multiplexed PCR to measure in situ growth rates of uropathogenic E. coli during experimental urinary tract infection | Applied and Environmental Microbiology

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Sep 8

Multiplexed PCR to measure in situ growth rates of uropathogenic E. coli during experimental UTI journals.asm.org/doi/10.1128/... @proteusqueen.bsky.social This study provides a cost-effective/efficient alternative to WGS for studying bacterial replication dynamics during infection.

Accurately measuring bacterial growth rates in the host, which plays a crucial role in determining the success of pathogens in establishing infections, poses significant challenges. To address this, b...

Multiplexed PCR to measure in situ growth rates of uropathogenic E. coli during experimental urinary tract infection | Applied and Environmental Microbiology

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Aug 5

Multiplexed PCR to measure in situ growth rates of uropathogenic E. coli during exp. UTI journals.asm.org/doi/10.1128/... @proteusqueen.bsky.social Cost-effective/efficient alt. for studying bacterial replication during infection, offering new info re pathogen behavior/host-microbe interactions.

Accurately measuring bacterial growth rates in the host, which plays a crucial role in determining the success of pathogens in establishing infections, poses significant challenges. To address this, b...

Adhesin Als4112 promotes Candida auris skin colonization through interactions with keratinocytes and extracellular matrix proteins

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Jul 7

pmc.ncbi.nlm.nih.gov/articles/PMC... @teresaomeara.bsky.social Study highlights the critical role of Als4112 in C. auris colonization and virulence, and explores potential strategies to reduce the pathogen’s adherence to abiotic surfaces and thus its spread in healthcare settings.

Candida auris is a fungal pathogen notorious for persistent skin colonization and transmission in healthcare settings. Here, we show that a C. auris conserved adhesin, Als4112, is required for skin co...

pmc.ncbi.nlm.nih.gov

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Jun 26

Temporal gating of nuclear import: How Merkel cell polyomavirus exploits the cell cycle for nuclear entry

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Jun 16

@bmoore-beth.bsky.social We are delighted to announce that Dr. Teresa O'Meara ‪was awarded the Henry Russel award at U-M. Our highest honor for an early-mid career faculty member for her outstanding research and teaching! @teresaomeara.bsky.social ‬ @umichmicroimmuno.bsky.social

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U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Jun 16

Checkout this article I found at PLOS: dx.plos.org/10.1371/jour... @drsprggs.bsky.social Highlights a key difference btw MCPyV & other polyomaviruses that will improve our understanding of its pathogenesis & aid in the development of targeted therapeutic strategies vs MCPyV-associated malignancies.

Author summary Merkel cell polyomavirus (MCPyV) causes a rare but deadly form of skin cancer called Merkel cell carcinoma. However, many aspects of its infectious life cycle are unclear, including how...

dx.plos.org

Nephrotoxicity of Intravenous Ganciclovir in Pediatric... : The Pediatric Infectious Disease Journal

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Jun 16

journals.lww.com/pidj/fulltex... @jason-weinberg.bsky.social Ganciclovir should be used with caution and close monitoring. Approaches to mitigate the risks of nephrotoxicity should be investigated.

ion. Methods: We identified children undergoing hematopoietic cell transplantation in the Pediatric Health Information System database and tracked them for 1 year following transplantation. The pri...

journals.lww.com

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Jun 9

www.sciencedirect.com/science/arti... @teresaomeara.bsky.social @drbadbug.bsky.social Overall, our findings illustrate non-canonical IRE1α activation during infection and a function for IRE1α in supporting organelle contact sites to safeguard against rapidly growing microbes.

IRE1α promotes phagosomal calcium flux to enhance macrophage fungicidal activity

The mammalian endoplasmic reticulum (ER) stress sensor inositol-requiring enzyme 1α (IRE1α) is essential for cellular homeostasis and plays key roles …

Deep homology and design of proteasome chaperone proteins in Candida auris

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U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Jun 9

pmc.ncbi.nlm.nih.gov/articles/PMC... @teresaomeara.bsky.social Study prospectively designed de novo chaperones and demonstrate that artificial proteins can rescue complex biological processes in the context of the whole cell.

A central tenet of biology is that protein structure mediates the sequence-function relationship. Recently, there has been excitement about the promise of advances in protein structure modeling to gen...

pmc.ncbi.nlm.nih.gov

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U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Jun 9

Macropinosomes are a site of HIV-1 entry into primary CD4+ T cells | PNAS www.pnas.org/doi/10.1073/... @kingrasa1.bsky.social Altogether, this study identifies macropinocytosis as one pathway for HIV-1 entry into primary CD4+ T cells.

Macropinosomes are a site of HIV-1 entry into primary CD4+ T cells | PNAS

HIV-1 has been observed to enter target cells at both the plasma membrane and endosomes. However, which pathways mediate its entry into primary CD4...

www.pnas.org

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · May 20

www.sciencedirect.com/science/arti... @drbadbug.bsky.social @teresaomeara.bsky.social Findings illustrate non-canonical IRE1α activation during infection and a function for IRE1α in supporting organelle contact sites to safeguard against rapidly growing microbes.

IRE1α promotes phagosomal calcium flux to enhance macrophage fungicidal activity

The mammalian endoplasmic reticulum (ER) stress sensor inositol-requiring enzyme 1α (IRE1α) is essential for cellular homeostasis and plays key roles …

Acinetobacter Baumannii Secreted Protease CpaA Inhibits Factor XII-Mediated Bradykinin Generation and Neutrophil Activation | Circulation Research

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · May 20

www.ahajournals.org/doi/10.1161/... @drbadbug.bsky.social A role for FXII in responding to bac. infection & suggests that by inhibiting the contact & KKSs & impairing neutrophil activation, CpaA may blunt the innate immune response/help prevent the elimination of A baumannii from the human host.

BACKGROUND: FXII (coagulation factor XII) is best known for its roles in the contact and kallikrein-kinin pathways. FXII is converted to its active enzyme (FXIIa [activated factor XII]) by PKa (plasma...

www.ahajournals.org

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U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · May 12

www.sciencedirect.com/science/arti... @drbadbug.bsky.social @teresaomeara.bsky.social Overall, our findings illustrate non-canonical IRE1α activation during infection and a function for IRE1α in supporting organelle contact sites to safeguard against rapidly growing microbes.

IRE1α promotes phagosomal calcium flux to enhance macrophage fungicidal activity

The mammalian endoplasmic reticulum (ER) stress sensor inositol-requiring enzyme 1α (IRE1α) is essential for cellular homeostasis and plays key roles …

Metabolic adaptability and nutrient scavenging in Toxoplasma gondii: insights from ingestion pathway-deficient mutants | mSphere

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U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · May 5

Metabolic adaptability and nutrient scavenging in Toxoplasma gondii: insights from ingestion pathway-deficient mutants MSphere journals.asm.org/doi/10.1128/... @verncarruthers.bsky.social This work advances our understanding of the metabolic adaptability of Toxoplasma.

Toxoplasma gondii is an obligate intracellular pathogen that infects virtually any nucleated cell in most warm-blooded animals. Infections are asymptomatic in most cases, but people with weakened immu...

HIV-1 single-transcription start-site mutants display complementary replication functions that are restored by reversion | Journal of Virology

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U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Apr 22

HIV-1 single-transcription start-site mutants display complementary replication functions restored by reversion journals.asm.org/doi/full/10.... @telesnitsky.bsky.social When mutants that expressed only one RNA were forced to revert, they regained functions associated with the second RNA.

HIV-1 generates two RNAs during its replication that differ by only two nucleotides in length. Despite this very minor difference, the RNAs perform different and complementary replication functions. W...

Metabolic adaptability and nutrient scavenging in Toxoplasma gondii: insights from ingestion pathway-deficient mutants | mSphere

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Apr 8

Metabolic adaptability/nutrient scavenging in T gondii: insights from ingestion pathway-deficient mutants Msphere journals.asm.org/doi/10.1128/... @verncarruthers.bsky.social This work advances our understanding of the metabolic adaptability of Toxoplasma.

Toxoplasma gondii is an obligate intracellular pathogen that infects virtually any nucleated cell in most warm-blooded animals. Infections are asymptomatic in most cases, but people with weakened immu...

Lung CD4+ resident memory T cells use airway secretory cells to stimulate and regulate onset of allergic airway neutrophilic disease

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Mar 31

www.sciencedirect.com/science/arti... @anukulshenoy.bsky.social Using our model of neutrophilic asthma, we identify lung epithelial-CD4+ TRM cell crosstalk as a key rheostat of allergic airway neutrophilia.

Neutrophilic asthma is a vexing disease, but mechanistic and therapeutic advancements will require better models of allergy-induced airway neutrophili…

JCI Insight - CD103+ dendritic cell–fibroblast crosstalk via TLR9, TDO2, and AHR signaling drives lung fibrogenesis

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Mar 31

JCI Insight - CD103+ dendritic cell–fibroblast crosstalk via TLR9, TDO2, and AHR signaling drives lung fibrogenesis insight.jci.org/articles/vie... @bmoore-beth.bsky.social For the first time to our knowledge, evidence that AHR exon 2 floxed mice retain the capacity for ncAHR signaling.

insight.jci.org

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Mar 31

Toxoplasma gondii PROP1 is critical for autophagy and parasite viability during chronic infection MSphere journals.asm.org/doi/full/10.... @verncarruthers.bsky.social Results reveal a critical role for TgPROP1 in autophagy and underscore the importance of this pathway in parasite persistence.

Toxoplasma gondii PROP1 is critical for autophagy and parasite viability during chronic infection | mSphere

It is estimated that up to a third of the human population is chronically infected with Toxoplasma gondii; however, little is known about how this parasite persists long term within its hosts. Autopha...

A membrane lipid signature unravels the dynamic landscape of group 1 innate lymphoid cells across the health-disease continuum

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Mar 24

www.sciencedirect.com/science/arti... @verncarruthers.bsky.social The detection of AsGM1 prior to Eomes expression, and its persistence in known transitional states, positions AsGM1 as a pivotal marker for tracing NK-to-ILC1 transitions, transcending ambiguity inherent to the NK-to-ILC1 continuum.

In an era where established lines between cell identities are blurred by intra-lineage plasticity, distinguishing stable from transitional states is c…

Environmental and Molecular Mutagenesis | EMGS Journal | Wiley Online Library

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Mar 18

onlinelibrary.wiley.com/doi/10.1002/... @jason-weinberg.bsky.social Mutagenicity is not considered a plausible initial key event in the mode of action for styrene-induced mouse lung tumors as these data support that styrene is not an in vivo mutagen.

Styrene has been shown to induce lung tumors in mice, but not in rats. The current study investigated the potential role of genotoxicity as an initial key event in the mode of action for styrene-indu....

onlinelibrary.wiley.com

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Mar 10

pubmed.ncbi.nlm.nih.gov/40035516/ @telesnitsky.bsky.social Despite this very minor difference, the RNAs perform different and complementary replication functions. When mutants that expressed only one RNA were forced to revert, they regained functions associated with the second RNA.

HIV-1 single-transcription start-site mutants display complementary replication functions that are restored by reversion - PubMed

HIV-1 transcription initiates at two positions, generating RNAs with either <sup>cap</sup>1G or <sup>cap</sup>3G 5' ends. The replication fates of these RNAs differ, with viral particles encapsidating...

pubmed.ncbi.nlm.nih.gov

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Mar 10

pubmed.ncbi.nlm.nih.gov/40053607/ @drbadbug.bsky.social Results highlight Z-DNA and ZBP1 as critical mediators for UVB-induced inflammation and uncover how type I IFNs prime for cutaneous inflammation in photosensitivity.

Epidermal ZBP1 stabilizes mitochondrial Z-DNA to drive UV-induced IFN signaling in autoimmune photosensitivity - PubMed

Photosensitivity is observed in numerous autoimmune diseases and drives poor quality of life and disease flares. Elevated epidermal type I interferon (IFN) production primes for photosensitivity and e...

pubmed.ncbi.nlm.nih.gov

A call for the United States to continue investing in science | mSphere

U. Mich Micro-Immuno Dept @umichmicroimmuno.bsky.social · Mar 4

A call for the United States to continue investing in science | mSphere journals.asm.org/doi/full/10.... @patschloss.bsky.social

The U.S. life science research mission is critical not only to human health and understanding the natural world but also to agriculture and food production, technological innovations, socioeconomic pr...