Thank you Minya!

Thank you Ali!

Thank you Sarah!

Thank you Jim!

Thank you Bob!

Thank you Sebastián!

Thank you Arif!

Thank you very much Jennifer!

Thank you Jose! I have just forwarded the full manuscript to you.

Thank you to all authors and collaborators on this project. This work would not have been possible without your hard work, insights, and fantastic teamwork.

Finally, five additional PME mRNAs were found to localize in the nucleus, and this bimodal pectin methylesterification is seen across several plant species. We propose that spatiotemporal cell wall patterning regulates stem cell dynamics and shoot meristem development.

During cell division, nuclear envelope breakdown (NEBD) releases PME5 mRNA for translation in the cytoplasm, driving active demethylesterification of new cross wall pectins, whereas nuclear sequestration of PME5 mRNA maintains high methylesterification in the surrounding walls.

Nuclear sequestration of protein-coding mRNA is unusual. But PME5 is not alone: our prior work also revealed nuclear localization of CDC20 and CCS52B (plant CDH1 ortholog) mRNAs, both encoding key cell cycle regulators (www.sciencedirect.com/science/arti...).

During the analysis of pectin modification, we found that mature wall is highly methylesterified while new cross wall is de-esterified. We discover that PME5, which removes the methyl group, is only expressed in dividing cells, and its mRNA is strictly sequestered in the nucleus.

Recently we showed that CSLD5 proteins, together with CESAs, rapidly accumulate at the cell division plane, contributing to robust wall synthesis and cell plate formation (www.nature.com/articles/s41...).

About a decade ago we began to look at the walls of stem cells by systematically analysing the expression patterns of glycosyltransferase (GTs) genes and polysaccharide composition in the shoot apical meristem (SAM） (www.cell.com/current-biol....

Thanks a lot Juan.

🤩 BEAUTIFULL highlight of our work by Sabine Muller now published in @currentbiology.bsky.social

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doi.org/10.1016/j.cu...