This system excels at creating large genomic fragment deletions, making it a powerful tool for knocking out non-coding regulatory elements. These edits are stably inherited by the next generation.

The performance jump is staggering. At single-site targets, Ymu1-WFR boosted editing efficiency by up to 9.8x compared to the wild-type. When integrated into their optimized "HDV+tRNA" array, they achieved average editing efficiencies of 41.3% to 48.9% at specific loci.

While the original TnpB (Ymu1) proved the concept, the team engineered a high-activity variant called Ymu1-WFR to push the limits. It’s designed specifically to overcome the low-efficiency hurdles typical of early-stage microbial nucleases.

By combining an engineered TnpB variant (Ymu1-WFR) with a TRV viral delivery system, they’ve boosted editing efficiency by nearly 10x! 📈
This "Swiss Army Knife" approach overcomes superinfection exclusion, allowing for multiple precision edits in a single go. Stable, heritable, and DNA-free. 🛠️🌱