#Cytometry
LightNews LightNews
acelineart.bsky.social
Going through one of my old notebooks for details about flow cytometry, and instead of finding that i just see 1500 doodles of my oc pls;........😭
November 7, 2025 at 7:07 PM
lovekat41.bsky.social
Back to the Alamos lab..
Post Cold War-
In 1969, Los Alamos reported the first fluorescence detector apparatus, which accurately measured the number and size of ovarian cells and blood cells, using flow cytometry.
Expanding research from nuclear to medical with research on Cancer,HIV, and vaccines.
November 7, 2025 at 7:50 PM
labliston.bsky.social
It is not often that a new flow cytometry protocol makes the newspaper! I also got a kick out of seeing our science tagged as "quirky"!

www.cambridgeindependent.co.uk/news/fairy-l...
Fairy Liquid unveiled as secret ingredient in University of Cambridge scientists’ near-universal magic formula
It helps them with experiments studying the inside of cells.
www.cambridgeindependent.co.uk
November 5, 2025 at 10:35 AM
acelineart.bsky.social
The flow cytometry station yearns for the doodles
November 7, 2025 at 7:25 PM
davidrach.bsky.social
Happy to officially announce that we are hosting a free “Cytometry in R” weekly mini-course, aimed at coding beginners, both in-person here at the University of Maryland, Baltimore and virtually, starting up in December/January. For details: umgccfcss.github.io/CytometryInR/ #rstats #flowcytometry
Course banner
November 5, 2025 at 9:36 PM
castltrastondrs.bsky.social
This suggests a persistent immune dysregulation in the #postCOVID19 era , which may contribute to long-term health effects.
@bmc.springernature.com
link.springer.com/article/10.1...
🔑 findings from Flow cytometry reveals a unique apoptotic phenotype that could serve as a biomarker
Investigating apoptosis in peripheral blood mononuclear cells among the elderly in the post-COVID-19 era - BMC Immunology
Background and aim The COVID-19 pandemic has left a lasting imprint on immune function, particularly in the elderly—a population already vulnerable to immunosenescence. While acute and long-COVID immu...
link.springer.com
November 6, 2025 at 5:20 AM
ekatrukha.bsky.social
How a trick from flow cytometry can help colocalization visualization for microscopy forum.image.sc/t/cytofluoro...
The image shows two cytofluorograms for the same two images, the left one built with linear axes and the right one with logarithmic axes.
October 31, 2025 at 10:32 AM
gemnov.bsky.social
Yesterday I gave a talk titled "Dividi et impera: Flow-Cytometry for FACS-based CRISPR screens and beyond" during the Flow Cytometry course organised by @embl.org & EMBLRome facilities. It was nice connecting w/ other users & the specialists from the facilities to hear the latest. FC & #FACS rock 🌈
October 30, 2025 at 6:11 PM
enirenberg.bsky.social
I don't think I've ever seen a lymphocyte gate quite like this one
flow cytometry plot showing a polygon covering essentially everything except for the highest side scatter cells and labeling all of them lymphocytes
October 30, 2025 at 9:05 PM
labliston.bsky.social
This paper did the hard-yards of testing various different blocking and signal preservation protocols. For any flow cytometry researchers a thorough read could save months of troubleshooting.

currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
Optimization of the Blocking and Signal Preservation Protocol in High‐Parameter Flow Cytometry
High quality input data is the key to successful interpretation of any scientific assay. In flow cytometry, fluorescently-conjugated antibodies allow us to simultaneously measure an incredible range ...
currentprotocols.onlinelibrary.wiley.com
October 31, 2025 at 9:09 AM
labliston.bsky.social
Huge credit to @colibricytometry.bsky.social, who has spent years working on every aspect of the spectral flow cytometry pipeline. Follow the Colibri blog to get tips in real-time, pre-pre-print!

www.colibri-cytometry.com
Cytometry Colibri | flow cytometry
Cytometry Colibri offers scientific consulting, data analysis, panel design, method development, tutoring and all things related to flow cytometry. Expertise in high parameter flow cytometry, spectra...
www.colibri-cytometry.com
October 28, 2025 at 8:06 AM
biorxiv-immuno.bsky.social
AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level https://www.biorxiv.org/content/10.1101/2025.10.27.684855v1
October 28, 2025 at 2:16 AM
ndevitomd.bsky.social
Extremely useful for spectral flow cytometry enthusiasts- thanks @labliston.bsky.social I will share with our core too
labliston.bsky.social Adrian Liston @labliston.bsky.social · 13d
Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold.

Want to get a tough sample, like lung, looking like this? Read more!

www.biorxiv.org/content/10.1...
October 28, 2025 at 3:22 PM
plosbiology.org
What are the #antiviral barriers to cross-species transmission of #zoonotic #influenza A viruses? @jordanbeckerphd.bsky.social @langloislab.bsky.social &co show that CpG-enriched avian #viruses are restricted by several mammalian proteins, including ZAP & KHNYN @plosbiology.org 🧪 plos.io/47fkMVp
Prenylated human ZAP and mammalian KHNYN can independently restrict naturally CpG-enriched ROSV. Top: CpG content of retroviruses (ROSV in black, ROSV-mCherry reporter virus in pink dashed line, MLV in orange, and HIV-1 in blue) calculated as # CpG per 100 nucleotides over a sliding window (graph). Schematic of retrovirus open reading frame (ORF) organization (top). Bottom left: Retroviral assembly and single-cycle infectivity results of human ZAP-L, ZAP-S, and mutants effects on ROSV showing relative percent ROSV infected cells (% mCherry-positive; % mCh+) as determined by flow cytometry. DF-1 cells transfected with increasing plasmid amounts of indicated host proteins (100, 200, or 400 ng), 1,500 ng ROSV, and 200 ng VSV-G. Infectivity is relative to backbone pcDNA3.2 transfections (Mock). Below, western blots detecting ROSV cleaved Gagp19 in supernatant virions as well as full-length ROSV GagPr76, ZAP proteins, and ACTIN loading controls from cellular lysates. Bottom right: Retroviral assembly and single-cycle infectivity results of human KHNYN, mutants, and mammalian KHNYN homologs (from pig, dog, and platypus) on ROSV infectivity. Below, western blots detecting ROSV cleaved Gagp19 in supernatant virions as well as full-length ROSV GagPr76, human KHNYN proteins, and ACTIN loading controls from cellular lysates. Note mammalian KHNYN orthologues were not detected by human KHNYN antibodies.
October 29, 2025 at 10:10 AM
simoncleary.bsky.social
Assaying lung samples using spectral flow cytometry? Check out AutoSpectral:
labliston.bsky.social Adrian Liston @labliston.bsky.social · 13d
Pre-print alert! And this one really is a must read for anyone that does spectral #flowcytometry. It is a complete, fully-automated spectral unmixing pipeline that reduces error up to 9000-fold.

Want to get a tough sample, like lung, looking like this? Read more!

www.biorxiv.org/content/10.1...
October 28, 2025 at 8:25 AM
drbradbrad.bsky.social
Postdoc vacancy: Microbial dormancy in the cryosphere
@erc.europa.eu #ERC_SIESTA
📢 PLEASE RT

🧬 Single cell microbial activity measurements, flow cytometry, cell sorting, omics, ecological interpretation

☀️ Marseille, France
‼️ Apply ASAP & before 7 Nov
Link: emploi.cnrs.fr/Offres/CDD/U...
October 21, 2025 at 3:48 PM
castltrastondrs.bsky.social
#Medsky🧪 #IDsky #oncosky #immunosky #publichealth Using spectral flow cytometry, researchers have defined the metabolic profiles of antigen-specific CD8+ T cells in various contexts, from infections to cancer . This method has been applied to analyze #Tcells responding to viruses like
October 21, 2025 at 4:25 AM
cypathlung.bsky.social
Day 2 at #CHEST2025! Great conversations with clinicians & researchers advancing noninvasive lung diagnostics.
Our team at Booth 2242 is sharing how #CyPathLung uses flow cytometry + #AI to support earlier, more accurate detection of lung cancer.
#bioAffinityTechnologies #LungHealth #Diagnostics
October 21, 2025 at 8:54 PM
swaledalemutton.bsky.social
I stopped being a postdoc back in 2007, but still do a fair amount of cytometry training in various labs, and I always brace myself if the PI wants to join in...
October 12, 2025 at 4:19 PM
sasscarpone.bsky.social
no one ever makes flow cytometry jokes ‼️
dsamorod.bsky.social Dan Samorodnitsky @dsamorod.bsky.social · Sep 26
Unlike the naysayers in the commentariat I think this is a good idea. Buy a used centrifuge coated with radioactivity on eBay. Drink ethidium bromide. Set up a flow cytometer where your baby's crib used to be. Go crazy buds.
September 26, 2025 at 1:48 AM
jcamthrash.bsky.social
Interested in removing particle-associated microbes from particles for eg flow cytometry, culturing, or other single-cell microbiology purposes? Led by graduating PhD stdnt Jordan Coelho:

Detergent-based separation of microbes from marine particles journals.asm.org/doi/10.1128/... #jcampubs 🌊
September 25, 2025 at 4:29 PM
drtesutherland.bsky.social
We're looking for a Lead Scientist to join Flow Cytometry Centre in Aberdeen! Fantastic opportunity to deliver services, work with varied research groups, promote cytometry and further develop the facility. Aberdeen is a great place to live/work- APPLY NOW www.abdnjobs.co.uk/vacancy/cyto...
Cytometry Facility Lead Scientist (IMS313A) | University of Aberdeen
A Lead Cytometry expert post is available for a highly motivated flow cytometrist to deliver cytometry services, promote and develop the Iain Fraser Cytometry Centre (IFCC).  This post is for a highly...
www.abdnjobs.co.uk
September 25, 2025 at 7:19 AM
biocompare.bsky.social
How can we measure the functionality of the innate immune system at scale? Join our webinar to learn how a novel omics assay using spectral flow cytometry generates thousands of readouts to link innate function with vaccine responses.

Register here: www.biocompare.com/620762-A-Nov...
September 22, 2025 at 12:23 PM
natmicrobiol.nature.com
#NewResearch

Using Salmonella strains genetically engineered to express minimal effector subsets and mass cytometry to characterise infection of the spleen, bottlenecks overcome by these effectors are revealed

#MicroSky 🦠

www.nature.com/articles/s41...
Single-cell analysis of genetically minimized Salmonella reveals effector gene cooperation in vivo - Nature Microbiology
A minimal effector subset enables Salmonella Typhimurium to overcome bottlenecks regulated by the early innate immune response and establish infection within a CD62L+ monocyte niche in the spleen.
www.nature.com
September 12, 2025 at 3:43 PM
drbradbrad.bsky.social
2 Postdoc vacancies: Microbial dormancy in the cryosphere
@erc.europa.eu #ERC_SIESTA
📢 PLEASE RT

Experimental:
🧬 Single cell microbial activity measurements, flow cytometry, omics, biogeochem

Modelling:
🖥️ Bioenergetics, thermodynamics, ecological, biogeochem

☀️ Marseille, France
‼️ Apply by 30 Sept
September 10, 2025 at 8:11 AM