#NatMethods
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Subcellular resolution (333 nm) Imaging Mass Cytometry🤠

Oversampling + point spread function image deconvolution

Executable on Hyperion XTi or Hyperion+ system

Resolving Nucleoli, neighboring B-T cells

25 plex Ab

#NatMethods 2025
www.nature.com/articles/s41...
November 1, 2025 at 10:54 AM
haoyin.bsky.social
#SpatialOmics

MCMICRO
Multiple Choice Microscopy pipeline
mcmicro.org

Multi-channel whole-slide images▶️
Single-cell data + Spatial Feature Tables

Multimodal (CyCIF CODEX mIHC) registration + HE IMC MIBI

#NatMethods 2022
www.nature.com/articles/s41...
October 12, 2025 at 12:21 PM
realbiomassspec.bsky.social
Uncovering hidden protein modifications with native top-down mass spectrometry #NatMethods www.nature.com/articles/s41...
Uncovering hidden protein modifications with native top-down mass spectrometry - Nature Methods
precisION discovers, localizes and quantifies protein modifications within complex proteoform assemblies through data-driven analysis of native top-down mass spectra.
www.nature.com
September 30, 2025 at 6:10 PM
realbiomassspec.bsky.social
MSnLib: efficient generation of open multi-stage fragmentation mass spectral libraries #NatMethods www.nature.com/articles/s41...
MSnLib: efficient generation of open multi-stage fragmentation mass spectral libraries - Nature Methods
MSnLib is a large-scale, open MSn spectral library featuring >2.3 million MSn and >357,000 MS2 spectra for 30,008 unique small molecules.
www.nature.com
September 16, 2025 at 3:05 PM
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🚨 New in Nat Methods: "Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing"

DOI: doi.org/10.1038/s415...

#Genomics #NatMethods #Transcriptomics #SDRseq #Science #SingleCell
Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing - Nature Methods
This study introduces SDR-seq, a droplet-based single-cell DNA–RNA sequencing platform, enabling the study of gene expression profiles linked to both noncoding and coding variants.
doi.org
September 9, 2025 at 9:04 AM
realbiomassspec.bsky.social
Unified mass imaging maps the lipidome of vertebrate development #NatMethods #MassSpec www.nature.com/articles/s41...
Unified mass imaging maps the lipidome of vertebrate development - Nature Methods
uMAIA is an analytical framework designed to enable the construction of metabolic atlases at high resolution using mass spectrometry imaging data.
www.nature.com
September 4, 2025 at 12:03 PM
realbiomassspec.bsky.social
A transformer model for de novo sequencing of data-independent acquisition mass spectrometry data #NatMethods www.nature.com/articles/s41...
A transformer model for de novo sequencing of data-independent acquisition mass spectrometry data - Nature Methods
Cascadia is a mass spectrometry-based de novo sequencing model that uses a transformer architecture to handle data-independent acquisition data and achieves substantially improved performance across a...
www.nature.com
July 2, 2025 at 1:25 PM
realbiomassspec.bsky.social
Empowering chemists to mine high-throughput mass spectrometry datasets #NatMethods www.nature.com/articles/s41...
Empowering chemists to mine high-throughput mass spectrometry datasets - Nature Methods
The Mass Query Language (MassQL) enables scientists to precisely express and reproducibly search for mass spectrometry (MS) peak patterns in large MS datasets. MassQL has been adopted across the most ...
www.nature.com
May 14, 2025 at 5:47 PM
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A universal language for finding mass spectrometry data patterns #NatMethods www.nature.com/articles/s41...
A universal language for finding mass spectrometry data patterns - Nature Methods
The Mass Spectrometry Query Language (MassQL) is an open-source language that enables instrument-independent searching across mass spectrometry data for complex patterns of interest via concise and ex...
www.nature.com
May 14, 2025 at 5:47 PM
haoyin.bsky.social
Spatial mechano-transcriptomics👹

Image-based cell mechanics inference
Segmentation Multi-junctional molecules▶️
Circular arc polygon tiling for cell contacts▶️
Cellular pressure, junctional tension, stress tensor

+Imaging-based #SpatialTranscriptomics

#NatMethods 2025
www.nature.com/articles/s41...
April 9, 2025 at 12:02 PM