This study presents an innovative Trapped Ion Mobility Spectrometry-Mass Spectrometry (TIMS-MS) approach employing rifampicin (RFP) and Ba2+ coordination chemistry to address the analytical challenges...

Structural isomerism in monounsaturated fatty acids (MUFAs) presents a persistent challenge in lipidomics due to limited chromatographic resolution and indistinguishable mass spectral profiles. Moreover, many methods that overcome these limitations require specialized instruments or complex photochemistry typically not accessible in for those using standard lipidomics core facilities. We report a streamlined LC-MS/MS method that integrates N-(4-aminomethylphenyl)pyridinium (AMP)-based charge-switch derivatization with meta-chloroperoxybenzoic acid (mCPBA) epoxidation to enable rapid and sensitive profiling of MUFA double bond positional isomers. Charge-switching enables enhanced detection of fatty acids while epoxidation improves chromatographic resolution and produces diagnostic fragmentation patterns upon collision-induced dissociation (CID), enabling precise localization of double bond positions. The optimized workflow achieves baseline separation of 16:1, 18:1, and 20:1 MUFA isomers within a 20-minute gradient, with reproducible retention times and consistent epoxide yields. The method also supports partial resolution of polyunsaturated fatty acids (PUFA) and cis/trans isomers. Application to prostate cancer cell lines revealed distinct MUFA isomer profiles associated with aberrant fatty acid desaturase 2 (FADS2) activity, including elevated n-10 and n-12 isomers in tumorigenic lines. These results align with prior studies using other isomer-resolving techniques, validating the method’s analytical performance. This accessible and robust strategy expands the toolkit for lipidomics research, providing a method that is compatible with conventional LC-MS/MS workflows, positioning it as a practical solution for high-resolution isomer analysis in complex biological samples

The human exposome features a highly expansive chemical space and substantial individual variability. Although screenings of xenobiotic compounds have revealed exposure landscapes of specific compound...

Terrestrial analog environments and their microbial communities are key elements to prepare for the exploration of extraterrestrial planetary environments that potentially sustained, or even may still...

INITIALISE was represented at the conference of Scandianavian Society of the Study of Diabetes(SSSD) Annual Meeting held on May 22-24, 2024 in Oulu, Finland. At the conference, new data on type 1 diab...

The exposome encompasses environmental exposures throughout life and significantly impacts health and disease. Exposure chemicals, present at trace levels, are frequently quantified using targeted LC–...

Bovine subclinical mastitis (SCM), an asymptomatic mammary inflammation, can lead to milk spoilage, premature culling of animals, and compromised milk quality. Our study aimed to identify and establis...

Monotropa uniflora L. (“ghost pipe”), is a North American traditional medicinal plant which has recently seen an increase in consumption nationwide. However, a single 1889 study reported the presence ...

Ion mobility-mass spectrometry has shown tremendous potential for improving the analysis of various subclasses of steroids. Its speed and ability to separate isobaric and isomeric species makes it…

Consumption of bioactive milk proteins enhances preterm infant development. Preterm infants are often supplemented with donor human milk (DHM) when parents’ own milk is unavailable in sufficient quantity. DHM is normally Holder pasteurized (HoP) to inactivate pathogens; however, it denatures many bioactive proteins. Alternatively, ultraviolet-C (UV–C) irradiation can inactivate pathogens while preserving milk proteins. This study examined the effects of HoP and UV–C on the proteome of DHM (n = 1 pool, processing in triplicate). Protein counts in HoP samples (n = 198) were lower than in raw DHM (n = 228) and UV–C at 6000 J/L (n = 239) samples. HoP resulted in significant reductions in relative abundance for 80 proteins (p-values <0.05, >0.5-fold decrease), whereas UV–C at 6000 J/L resulted in only 5 proteins significantly decreasing. Proteins reduced by HoP that were preserved by UV–C treatment had a broad array of functions, including antimicrobial, iron-binding, immunomodulatory, and proteolytic. Applying UV–C processing for donor milk processing could improve outcomes in preterm infants.

Harmful algal blooms (HABs) pose severe threats to aquatic ecosystems, yet rapid and accurate prediction of algal density remains challenging. As integrated metabolites are released throughout the alg...

Shell matrix proteins (SMPs) are fundamental biological macromolecules for mollusk shell formation, yet fewer than 400 SMPs in mollusks have been previously identified, hindering our understanding of ...

This study investigates the phenolic and fatty acid profiles of olives from four Olea europaea cultivars (Arbequina, Arbosana, Frantene, and Koroneiki), widely grown in the Mediterranean region and co...