I'm kind of fascinated with Oct4. That's the gene generally seen as a maintainer of pluripotency in stem cells. Crudely, as long as there's Oct4, the cells can stay pluripotent. It's one of the Yamanaka factors for reprogramming cells to iPSCs. So, a simple story? Get real: this is biology. 1/n
November 11, 2025 at 5:01 PM
I'm kind of fascinated with Oct4. That's the gene generally seen as a maintainer of pluripotency in stem cells. Crudely, as long as there's Oct4, the cells can stay pluripotent. It's one of the Yamanaka factors for reprogramming cells to iPSCs. So, a simple story? Get real: this is biology. 1/n
MIT researchers invent new human brain model to enable disease research, drug discovery news.mit.edu/2025/researc...
Cultured from induced pluripotent stem cells, “miBrains” integrate all major brain cell types and model brain structures, cellular interactions, activity, and pathological features
Cultured from induced pluripotent stem cells, “miBrains” integrate all major brain cell types and model brain structures, cellular interactions, activity, and pathological features
MIT researchers invent new human brain model to enable disease research, drug discovery
A new 3D human brain tissue platform developed by MIT researchers is the first to integrate all major brain cell types, including neurons, glial cells, and the vasculature, into a single culture. Grow...
news.mit.edu
November 8, 2025 at 1:37 PM
MIT researchers invent new human brain model to enable disease research, drug discovery news.mit.edu/2025/researc...
Cultured from induced pluripotent stem cells, “miBrains” integrate all major brain cell types and model brain structures, cellular interactions, activity, and pathological features
Cultured from induced pluripotent stem cells, “miBrains” integrate all major brain cell types and model brain structures, cellular interactions, activity, and pathological features
Professor Darrell Kotton takes the floor to talk to us about pluripotent stem cells in the era of respiratory genomics. #RespGen25
November 4, 2025 at 3:11 PM
Professor Darrell Kotton takes the floor to talk to us about pluripotent stem cells in the era of respiratory genomics. #RespGen25
Kidney organoids derived from human pluripotent stem cells are infused in a normothermic machine perfusion system to condition explanted porcine kidneys, and the feasibility and viability of their in vivo engraftment are demonstrated. www.nature.com/articles/s41...
November 4, 2025 at 12:56 AM
Kidney organoids derived from human pluripotent stem cells are infused in a normothermic machine perfusion system to condition explanted porcine kidneys, and the feasibility and viability of their in vivo engraftment are demonstrated. www.nature.com/articles/s41...
Marie, also known as Lab Skills Academy, walks us through how scientists turn cells into induced pluripotent stem cells, also known as iPSCs, and guide them into becoming cardiomyocytes: heart cells that beat in rhythm just like yours.
November 3, 2025 at 4:41 PM
Marie, also known as Lab Skills Academy, walks us through how scientists turn cells into induced pluripotent stem cells, also known as iPSCs, and guide them into becoming cardiomyocytes: heart cells that beat in rhythm just like yours.
Induced Pluripotent Stem Cells Explained
Induced pluripotent stem cells (iPSCs) are revolutionizing biology-but what are they, and why are they important?
🎧 Watch Episode #2 with Dr. Zafeiriou to explore #StemCells, #Organoids, and #RegenerativeMedicine.
youtube.com/shorts/QN58u...
Induced pluripotent stem cells (iPSCs) are revolutionizing biology-but what are they, and why are they important?
🎧 Watch Episode #2 with Dr. Zafeiriou to explore #StemCells, #Organoids, and #RegenerativeMedicine.
youtube.com/shorts/QN58u...
Induced Pluripotent Stem Cells Explained
YouTube video by Neuroscience and Beyond
youtube.com
November 2, 2025 at 5:11 PM
Induced Pluripotent Stem Cells Explained
Induced pluripotent stem cells (iPSCs) are revolutionizing biology-but what are they, and why are they important?
🎧 Watch Episode #2 with Dr. Zafeiriou to explore #StemCells, #Organoids, and #RegenerativeMedicine.
youtube.com/shorts/QN58u...
Induced pluripotent stem cells (iPSCs) are revolutionizing biology-but what are they, and why are they important?
🎧 Watch Episode #2 with Dr. Zafeiriou to explore #StemCells, #Organoids, and #RegenerativeMedicine.
youtube.com/shorts/QN58u...
How do pluripotent stem cells resist harmful interferon responses to safeguard development? Through total epigenetic lockdown of ligands, sensors and effectors of IFN-I. In our preprint, James Holt shares his PhD discoveries on the ground state of immune evasion 😊: www.biorxiv.org/cgi/content/...
Epigenetic lockdown of type I interferon sensing and signalling in human pluripotent cells.
The Human Silencing Hub (HUSH) complex safeguards genome integrity in human somatic cells by repressing transposable elements and regulating type I interferon (IFN-I) induction. In early development, ...
www.biorxiv.org
October 29, 2025 at 6:03 PM
How do pluripotent stem cells resist harmful interferon responses to safeguard development? Through total epigenetic lockdown of ligands, sensors and effectors of IFN-I. In our preprint, James Holt shares his PhD discoveries on the ground state of immune evasion 😊: www.biorxiv.org/cgi/content/...
📣 New preprint from the Jakobsson lab!
We describe a detailed protocol to inhibit the expression of transposable elements in human pluripotent stem cells, developed thanks to @asapresearch.parkinsonsroadmap.org funding.
Special shoutout to @anitaada.bsky.social!
www.biorxiv.org/content/10.1...
We describe a detailed protocol to inhibit the expression of transposable elements in human pluripotent stem cells, developed thanks to @asapresearch.parkinsonsroadmap.org funding.
Special shoutout to @anitaada.bsky.social!
www.biorxiv.org/content/10.1...
A framework for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cells
This protocol describes the workflow for transcriptional silencing of transposable elements (TEs) in human induced pluripotent stem cells (hiPSCs). The protocol illustrates how to design gRNAs to targ...
www.biorxiv.org
October 29, 2025 at 8:24 AM
📣 New preprint from the Jakobsson lab!
We describe a detailed protocol to inhibit the expression of transposable elements in human pluripotent stem cells, developed thanks to @asapresearch.parkinsonsroadmap.org funding.
Special shoutout to @anitaada.bsky.social!
www.biorxiv.org/content/10.1...
We describe a detailed protocol to inhibit the expression of transposable elements in human pluripotent stem cells, developed thanks to @asapresearch.parkinsonsroadmap.org funding.
Special shoutout to @anitaada.bsky.social!
www.biorxiv.org/content/10.1...
6/ Applying these insights to stem cell differentiation, we show that manipulating P-body assembly or specific microRNA activity can direct pluripotent stem cells toward totipotency or the germ-cell lineage.
October 28, 2025 at 4:02 PM
6/ Applying these insights to stem cell differentiation, we show that manipulating P-body assembly or specific microRNA activity can direct pluripotent stem cells toward totipotency or the germ-cell lineage.
A single sensory neuron grown from an induced pluripotent stem cell (iPSC), labeled for tubulin and actin to reveal its inner architecture.
🧠 This living model is used to study neurodevelopment, regeneration, and disease — how sensory pathways form, fail, and might be repaired.
#FluorescenceFriday
🧠 This living model is used to study neurodevelopment, regeneration, and disease — how sensory pathways form, fail, and might be repaired.
#FluorescenceFriday
October 24, 2025 at 3:47 PM
A single sensory neuron grown from an induced pluripotent stem cell (iPSC), labeled for tubulin and actin to reveal its inner architecture.
🧠 This living model is used to study neurodevelopment, regeneration, and disease — how sensory pathways form, fail, and might be repaired.
#FluorescenceFriday
🧠 This living model is used to study neurodevelopment, regeneration, and disease — how sensory pathways form, fail, and might be repaired.
#FluorescenceFriday
Day 2 of #EESOrgan:
Many different organoids,methods and applications!Highlights:"Generation of urinary tract organoids from human pluripotent stem cells"by Minoru Takasato&"Changing kidney Organoid formats for improved drug development and tissue engineering" by Melissa Little.Can't wait for Day 3!
Many different organoids,methods and applications!Highlights:"Generation of urinary tract organoids from human pluripotent stem cells"by Minoru Takasato&"Changing kidney Organoid formats for improved drug development and tissue engineering" by Melissa Little.Can't wait for Day 3!
October 23, 2025 at 4:10 PM
Day 2 of #EESOrgan:
Many different organoids,methods and applications!Highlights:"Generation of urinary tract organoids from human pluripotent stem cells"by Minoru Takasato&"Changing kidney Organoid formats for improved drug development and tissue engineering" by Melissa Little.Can't wait for Day 3!
Many different organoids,methods and applications!Highlights:"Generation of urinary tract organoids from human pluripotent stem cells"by Minoru Takasato&"Changing kidney Organoid formats for improved drug development and tissue engineering" by Melissa Little.Can't wait for Day 3!
Everything else aside…
How in the pluripotent FUCK does a candidate positioning themselves as a progressive champion for change get THIS FAR into the primary process without somebody else saying “dude that thing is a Liability. WTF were you thinking?”
How in the pluripotent FUCK does a candidate positioning themselves as a progressive champion for change get THIS FAR into the primary process without somebody else saying “dude that thing is a Liability. WTF were you thinking?”
October 21, 2025 at 8:48 PM
Everything else aside…
How in the pluripotent FUCK does a candidate positioning themselves as a progressive champion for change get THIS FAR into the primary process without somebody else saying “dude that thing is a Liability. WTF were you thinking?”
How in the pluripotent FUCK does a candidate positioning themselves as a progressive champion for change get THIS FAR into the primary process without somebody else saying “dude that thing is a Liability. WTF were you thinking?”
'This multicellular system with induced pluripotent stem cell-derived neurons, microglia, oligodendroglia, astrocytes, pericytes, and brain microvascular endothelial cells has potential for opening possibilities for disease modeling, drug discovery and development'
#Immunology #Neuroimmunology
#Immunology #Neuroimmunology
Engineered 3D immuno-glial-neurovascular human miBrain model | PNAS
Patient-specific, human-based cellular models integrating a biomimetic blood–brain
barrier, immune, and myelinated neuron components are critically...
www.pnas.org
October 18, 2025 at 5:04 PM
'This multicellular system with induced pluripotent stem cell-derived neurons, microglia, oligodendroglia, astrocytes, pericytes, and brain microvascular endothelial cells has potential for opening possibilities for disease modeling, drug discovery and development'
#Immunology #Neuroimmunology
#Immunology #Neuroimmunology
For #FluorescenceFriday - human iPSC-derived cerebral organoid - labeled w/ MAP2🟢, CTIP2🔴, PAX6⚪, Hoechst🔵 from our Molecular Neurogenetics team. Image credit to @tsawada1.bsky.social and JHU Human Genetics grad student Bailey Spiegelberg 🔬🧠🧪
October 17, 2025 at 5:17 PM
For #FluorescenceFriday - human iPSC-derived cerebral organoid - labeled w/ MAP2🟢, CTIP2🔴, PAX6⚪, Hoechst🔵 from our Molecular Neurogenetics team. Image credit to @tsawada1.bsky.social and JHU Human Genetics grad student Bailey Spiegelberg 🔬🧠🧪
Cultured from induced pluripotent stem cells, ‘miBrains’ integrate all major brain cell types and model brain structures, cellular interactions, activity, and pathological features. picower.mit.edu/news/mit-inv... #neuroscience
MIT invents human brain model with six major cell types to enable personalized disease research, drug discovery
Cultured from induced pluripotent stem cells, ‘miBrains’ integrate all major brain cell types and model brain structures, cellular interactions, activity, and pathological features.
picower.mit.edu
October 17, 2025 at 7:20 PM
Cultured from induced pluripotent stem cells, ‘miBrains’ integrate all major brain cell types and model brain structures, cellular interactions, activity, and pathological features. picower.mit.edu/news/mit-inv... #neuroscience
from workshop blueprints 2025:
Balcone by Trinkay
Balcone by Trinkay
October 15, 2025 at 9:12 PM
from workshop blueprints 2025:
Balcone by Trinkay
Balcone by Trinkay
Multi-Cellular Human Liver Organoids Enable Complete Maturation of Induced Pluripotent Hepatocyte-like Cells Through Purely Endogenous Signals https://www.biorxiv.org/content/10.1101/2025.10.09.681454v1
October 11, 2025 at 1:46 AM
Multi-Cellular Human Liver Organoids Enable Complete Maturation of Induced Pluripotent Hepatocyte-like Cells Through Purely Endogenous Signals https://www.biorxiv.org/content/10.1101/2025.10.09.681454v1
Honouring the life of Sir John Gurdon, who died earlier this week: awarded the Nobel Laureate in Physiology or Medicine for seminal work carried out in Oxford Zoology (now @biology.ox.ac.uk) showing that mature cells could be reprogrammed to become pluripotent.
www.nobelprize.org/prizes/medic...
www.nobelprize.org/prizes/medic...
Nobel Prize in Physiology or Medicine 2012
The Nobel Prize in Physiology or Medicine 2012 was awarded jointly to Sir John B. Gurdon and Shinya Yamanaka "for the discovery that mature cells can be reprogrammed to become pluripotent"
www.nobelprize.org
October 10, 2025 at 12:23 PM
Honouring the life of Sir John Gurdon, who died earlier this week: awarded the Nobel Laureate in Physiology or Medicine for seminal work carried out in Oxford Zoology (now @biology.ox.ac.uk) showing that mature cells could be reprogrammed to become pluripotent.
www.nobelprize.org/prizes/medic...
www.nobelprize.org/prizes/medic...
🍂 🧪 John Gurdon 1933-2025
2012 Nobel Prize in Physiology or Medicine
For pioneering work transferring nuclei into frog eggs, demonstrating that eggs can reprogram differentiated cell nuclei to a pluripotent, embryonic state
2012 Nobel Prize in Physiology or Medicine
For pioneering work transferring nuclei into frog eggs, demonstrating that eggs can reprogram differentiated cell nuclei to a pluripotent, embryonic state
Very sad to learn of the death of Sir John Gurdon. He made a huge contribution to developmental biology & should be an inspiration to anyone whose potential is dismissed in school. It was a pleasure to work with him when he was Chair of the COB Board. www2.mrc-lmb.cam.ac.uk/john-gurdon-...
John Gurdon (1933 – 2025) - MRC Laboratory of Molecular Biology
Renowned developmental biologist John Gurdon, former Group Leader and Head of the LMB’s Cell Biology Division, has died aged 92.
www2.mrc-lmb.cam.ac.uk
October 8, 2025 at 9:53 PM
🍂 🧪 John Gurdon 1933-2025
2012 Nobel Prize in Physiology or Medicine
For pioneering work transferring nuclei into frog eggs, demonstrating that eggs can reprogram differentiated cell nuclei to a pluripotent, embryonic state
2012 Nobel Prize in Physiology or Medicine
For pioneering work transferring nuclei into frog eggs, demonstrating that eggs can reprogram differentiated cell nuclei to a pluripotent, embryonic state
John Gurdon, former Group Leader and Head of @cellbiol-mrclmb.bsky.social has died.
Despite calling himself a "total non-intellectual” his work to reprogramme somatic cells to pluripotent stem cells transformed the field of developmental biology.
More: www2.mrc-lmb.cam.ac.uk/john-gurdon-...
#LMBNews
Despite calling himself a "total non-intellectual” his work to reprogramme somatic cells to pluripotent stem cells transformed the field of developmental biology.
More: www2.mrc-lmb.cam.ac.uk/john-gurdon-...
#LMBNews
October 8, 2025 at 8:49 AM
John Gurdon, former Group Leader and Head of @cellbiol-mrclmb.bsky.social has died.
Despite calling himself a "total non-intellectual” his work to reprogramme somatic cells to pluripotent stem cells transformed the field of developmental biology.
More: www2.mrc-lmb.cam.ac.uk/john-gurdon-...
#LMBNews
Despite calling himself a "total non-intellectual” his work to reprogramme somatic cells to pluripotent stem cells transformed the field of developmental biology.
More: www2.mrc-lmb.cam.ac.uk/john-gurdon-...
#LMBNews
"Surprisingly, individual cells—from splenic lymphocytes to pluripotent stem cells—transcribe only ∼0.02%–3.1% of the genome, versus >80% in bulk, revealing limited genome engagement and profound cell-type and cell-to-cell heterogeneity"
www.cell.com/cell/abstrac...
www.cell.com/cell/abstrac...
Single-cell nascent transcription reveals sparse genome usage and plasticity
scFLUENT-seq is a single-cell method that sensitively captures nascent nuclear transcriptomes
to reveal mRNA and ncRNA dynamics, heterogeneity across chromatin compartments, and
transcriptional divers...
www.cell.com
October 7, 2025 at 12:23 PM
"Surprisingly, individual cells—from splenic lymphocytes to pluripotent stem cells—transcribe only ∼0.02%–3.1% of the genome, versus >80% in bulk, revealing limited genome engagement and profound cell-type and cell-to-cell heterogeneity"
www.cell.com/cell/abstrac...
www.cell.com/cell/abstrac...
🪱 Planarian (Schmidtea mediterranea)
✨ Planarians can regenerate a complete body from tiny fragments thanks to their amazing pluripotent stem cells (neoblasts) 🧬 A star of regeneration and stem cell biology
📸 Image by Rachel Gandee #ModelMonday #DevBio
📸 Image by Rachel Gandee #ModelMonday #DevBio
October 6, 2025 at 4:42 PM
🪱 Planarian (Schmidtea mediterranea)
✨ Planarians can regenerate a complete body from tiny fragments thanks to their amazing pluripotent stem cells (neoblasts) 🧬 A star of regeneration and stem cell biology
📸 Image by Rachel Gandee #ModelMonday #DevBio
📸 Image by Rachel Gandee #ModelMonday #DevBio
Many enhancers that drive tissue-specific gene expression are already connected to gene promoters in human pluripotent cells.
In a new preprint, we share some clues about when, how, and why this happens!
www.biorxiv.org/content/10.1...
In a new preprint, we share some clues about when, how, and why this happens!
www.biorxiv.org/content/10.1...
Poising and connectivity of emergent human developmental enhancers in the transition from naive to primed pluripotency
In primed human pluripotent stem cells (hPSCs) resembling post-implantation epiblast, numerous lineage-specific enhancers assume the poised chromatin state, co-marked by H3K4me1 and Polycomb-associate...
www.biorxiv.org
October 3, 2025 at 3:09 PM
Many enhancers that drive tissue-specific gene expression are already connected to gene promoters in human pluripotent cells.
In a new preprint, we share some clues about when, how, and why this happens!
www.biorxiv.org/content/10.1...
In a new preprint, we share some clues about when, how, and why this happens!
www.biorxiv.org/content/10.1...
Nice work from the labs of @katemiro.bsky.social @sarawickstrom.bsky.social
Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells
www.nature.com/articles/s41...
Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells
www.nature.com/articles/s41...
Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells - Nature Cell Biology
McCreery, Stubb et al. show that mechano-osmotic changes in the nucleus induce general transcriptional repression and prime chromatin for cell fate transitions by relieving repression of specific differentiation genes.
www.nature.com
September 30, 2025 at 5:50 AM
Nice work from the labs of @katemiro.bsky.social @sarawickstrom.bsky.social
Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells
www.nature.com/articles/s41...
Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells
www.nature.com/articles/s41...
Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells @natcellbio.nature.com @sarawickstrom.bsky.social @akistubb.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
www.nature.com/articles/s41...
www.nature.com/articles/s41...
September 29, 2025 at 8:28 PM
Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells @natcellbio.nature.com @sarawickstrom.bsky.social @akistubb.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
www.nature.com/articles/s41...
www.nature.com/articles/s41...