#scATACseq
But how does this relate to human disease?? Through an awesome collaboration with the @anshulkundaje.bsky.social lab, we trained ChromBPNet models with scATACseq datasets for each cell type and vascular site, and predict human variant effect on a cell type/site basis @soumyakundu.bsky.social
September 10, 2025 at 3:54 PM
MuMu: a sample multiplexing protocol for droplet-based simultaneous single nuclei RNA- and ATAC-seq systems. #SingleNuclei #MultiplexingProtocol #10X #scRNAseq #scATACseq @biorxivpreprint.bsky.social
www.biorxiv.org/content/10.1...
December 1, 2024 at 7:41 PM
#Atherosclerosis #FoamCell heterogeneity 2/2

Meta-analysis 37 scRNAseq libraries + 44 scATACseq datasets->
5 Foam cell states

ICAM1+ inflammatory
FOLR2+ phagocytic
MMP9+ matrix-remodeling
ANGPTL4+ hypoxic
COL1A2+ fibroblastic

#Biomol_MDPI 2024
www.mdpi.com/2218-273X/14...
September 7, 2025 at 12:57 PM
6/
I also did early single-cell work—RNA-seq and ATAC-seq.
That experience paid off when I collaborated with Tao Liu, creator of MACS2 to work on scATACseq.
Tao was my hero during my PhD!
July 26, 2025 at 1:45 PM
September 11, 2025 at 8:31 AM
#SpatialOmics #MultiOMics

MaxFuse

Integration of spatial & single-cell data across modalities with weakly linked features

Fuzzy Smoothing
Iterative cycles of coembedding, graph-smoothing & matching

tri-omic scRNAseq scATACseq CODEX integration

#NatBiotech 2023
www.nature.com/articles/s41...
April 27, 2025 at 12:08 AM
5/ The Power of Single-Cell Experience
8 years ago, I worked on single-cell RNA-seq & ATAC-seq at Harvard FAS Informatics. At Dana-Farber, I collaborated with Tao Liu for scATACseq, creator of MACS2—the most popular peak caller. That experience was invaluable.
February 2, 2025 at 3:48 PM
Integrated #scATACseq and #scRNAseq data reveals that ATF6 plays a pivotal role in maintaining the homeostasis of lens epithelial cells and facilitates EMT in epithelial cells by regulating p-STAT3 expression. #SecondaryCataracts #Cataracts #medsky

#OpenAccess: buff.ly/UXLfbrp
March 30, 2025 at 10:30 PM
Alevin-fry 0.11.0 was just released (last night). This speed up permit-list generation by making it multithreaded, but much more importantly, adds the `atac` command allowing barcode correction and counting & BED generation directly in the main branch of alevin-fry. 1/3 #scRNAseq #scATACseq
December 7, 2024 at 2:46 PM
🚀 Sharing insights on ArchR, a powerful tool for single-cell chromatin accessibility analysis! 🎯 ArchR excels in large datasets and integrates scATACseq with scRNAseq for cutting-edge multi-modal studies.
🎥 Dive deeper with the video: youtu.be/XNZx5Dlp6jY?...
#Bioinformatics #10xGenomics #LIBD
[2024-12-11] ArchR journal club
YouTube video by Leonardo Collado Torres
youtu.be
December 12, 2024 at 3:01 PM
We also saw a shift in TF activity.

Footprinting showed increased NFI binding, especially NFIB, in late-born cohorts. These neurons activate maturation modules faster — possibly guided by chromatin remodeling during neurogenesis.
#TFfootprints #TranscriptionFactors #scATACseq
(5/n)
July 8, 2025 at 12:04 PM
Multiomic Atlas of Human Tonsil
scRNAseq
scATACseq
CITEseq

#SpatialTranscriptomics #Visium

Marginal Reticular Cells, not fibroblastic reticular cells, are high in PDGFRβ, #FibrillarCollagen, Proteoglycan core proteins & DIO2

#Immunity 2024
www.cell.com/immunity/ful...
February 1, 2024 at 12:53 PM
chatomics! PCA analysis on scATACseq data. In this blog post, I will show you how the first PC is correlated with sequencing depth divingintogeneticsandgenomics.com/post/pca-sc...
February 9, 2025 at 3:15 PM
MAGICAL Multiome Accessibility Gene Integration Calling & Looping

Work for true or sample-paired scRNAseq + scATACseq

Bayesian framework to learn hidden TF activity & generate high-confidence TF-peak-gene circuits

vs TRIPOD FigR

#NatComputSci 2023
www.nature.com/articles/s43...
May 29, 2024 at 11:49 AM
Human brain #Organoid
scRNAseq + scATACseq + single-cell transcription factor perturbation (CROP-seq, lentiviral gRNA library for 20 TFs, 3 gRNAs/TF, 1 gRNA/cell in >22k cells) in

GLI3-HES1 antagonism for dorsoventral patterning in human telencephalon

#Nature 2023
www.nature.com/articles/s41...
September 6, 2025 at 7:28 PM
Want to visualize & create a website for your omics data (incld CITEseq, scATACseq, #SpatialTranscriptomics)?

Try ShinyCell2!😆

Compatible with data formats Seurat Signac ArchR Scanpy

vs cellxgene WebAtlas Vitessce

github.com/the-ouyang-l...

bioRxiv 2024
www.biorxiv.org/content/10.1...
May 5, 2025 at 6:50 PM
Single-cell omics🐭#OvarianAging
scRNAseq scATACseq
12- vs 3-mo

#Aging
1⃣Mesenchymal Phox2B/ESRRγ/HoxB7-TGFβ2➡️ #Fibrosis (steroidogenic stroma, fibroblast, theca, but NOT pericyte)
2⃣Granulosa cell HSPA5 #ATF3 ATF4➡️#ERStress

Any #VascularAging?

#GeroScience 2025
link.springer.com/article/10.1...
March 11, 2025 at 1:09 AM
Great conversations and inspiring vibes at @events.embl.org — felt amazing to share my work and hear from incredible speakers!
#EESAgeing #SingleCell #Aging #ATACseq #RNAseq #Immunology #Multiomics #scRNAseq #scATACseq
June 11, 2025 at 3:45 PM
How is the progression of maturation regulated in forebrain inhibitory neurons? Excited to share our new study from the Mayer lab — stable differentiation, dynamic maturation, and stage-specific chromatin changes in GABAergic neurons.

#GABA #Neuroscience #scATACseq #scRNAseq
July 9, 2025 at 6:20 AM
This shift was mirrored in chromatin accessibility.

scATAC-seq revealed stage-specific enhancer activation, with late-born cells opening distinct regulatory elements — especially in distal regions. A dynamic change in the regulatory landscape.
#Chromatin #Epigenetics #scATACseq
(4/n)
July 8, 2025 at 12:04 PM
🔬 Your immune system doesn’t just weaken with age—it evolves. We mapped how.
📊 We used single-cell multi-omics (RNA + ATAC) + bulk ATAC on PBMCs to build a cell-by-cell map of aging.
👉 Poster 83, EMBO | EMBL Symposium
#SingleCell #Aging #EMBL #Multiomics #scRNAseq #scATACseq
June 10, 2025 at 10:39 AM
Superb work by Wouter @Zouters & @OlgaPushkarev developing ChromatinHD, two scale-adaptive #machinelearning models & interpretation tools that use #scRNAseq + #scATACseq data to better understand how chromatin accessibility relates to gene expression doi.org/10.1038/s414...

Happy 2025 everyone 🎆 !
ChromatinHD connects single-cell DNA accessibility and conformation to gene expression through scale-adaptive machine learning - Nature Communications
Functional chromatin changes occur at different scales. Here, the authors introduce ChromatinHD, a method that characterises differential and predictive chromatin accessibility changes in a scale-adap...
doi.org
January 6, 2025 at 2:05 PM
Hi ! I am new here and I like to present my interests with this video from our Journal Club! This is an overview of the multiome analysis for scATACseq and scRNAseq using several
#CellRanger pipelines. This is inspired by our work at #LIBD and resources from 10x Genomics. youtu.be/fHVLi9-sftM?...
[2024-10-11] An overview of the CellRanger-ARC Pipeline Analysis
YouTube video by Leonardo Collado Torres
youtu.be
November 8, 2024 at 8:29 PM
Spatially-resolved MultiOmics of🐭fetal #Cardiomyocyte
snRNAseq scATACseq 10X Visium
13 CM subsets
NFYa marks CM4-like subsets

CM NFYa KO🐭->
Embryonic death
#CardiacNoncompaction/Hypertrabeculation
⏬Proliferation
⏬Mitochondrial respiration

#DevCell 2023
www.sciencedirect.com/science/arti...
December 2, 2023 at 8:08 PM