Optimization starts with proper generation & handling of spheroids.

Tumour development and progression are associated with biophysical alterations that manifest across multiple spatial scales, from the subcellular to multicellular tissue scale. While cells can dynamic...

The success of chimeric antigen receptor (CAR) T cells in blood cancers has intensified efforts to develop CAR T therapies for solid cancers. In the solid tumor

This preclinical study suggests that tumor iso-efficacy with UHDR may not hold true for all cancer types and is associated with oxygen level.

A wireless skin-wound care platform for photothermal-stem cell-light (PTSCL) therapy, introduced here, leverages a fully wireless and battery-free optoelectronic module, gold nanoturf-mediated photothermal interface, and human adipose-derived stem cell (hADSC) spheroids to synergistically enhance wound healing. Besides accelerated skin wound closure, the proposed system shows efficacy regarding cell delivery, skin maturation, and angiogenesis. Abstract 3D stem cell aggregates at the sub-millimeter feature, known as spheroids, have garnered significant interest for their capability to effectively heal and close wound areas. Nevertheless, further efforts to unlock their maximal therapeutic potential and to address engineering challenges are required for medical/clinical translation. Herein, a wireless skin wound care platform designed for multimodal therapy, combining photothermal, stem cell, and light (PTSCL) effects to enhance therapeutic efficacy, is presented. The synergy is achieved by integrating a wireless LED module that irradiates visible light (630 nm-wavelength) and human adipose-derived stem cells (hADSCs) spheroid-laden polydimethylsiloxane (PDMS) patch embedded with a light-to-heat converting membrane (i.e., Au-coated nanoturf). This wireless platform primarily enables photothermal stimulation at the wound site, facilitating hADSC spheroid formation and upregulation via heat shock protein-mediated mechanism, and effective delivery of spheroids from the patch. Additionally, the photothermal approach, comprising mild hyperthermia and light stimulation, independently utilized to promote wound healing, exhibits a synergistic therapeutic effect. Collected data from visual/computational analysis, in vitro assays, and in vivo experiments in a mouse wound model (2 cm-by-2 cm) provide evidence for the synergistic effect of the proposed PTSCL treatment and suggest its potential to be implemented for tissue regeneration for severe wound patients.