It is postulated that the PDB should use the CAVEAT record more prominently to warn scientists using its archives of potential risks and errors.

The construction of a new experimental station for macromolecular crystallography at NanoTerasu under the AMED/BINDS project is presented.

This work demonstrates that multi-position data acquisition mitigates radiation damage in electron diffraction of three-dimensional protein crystals and that merging data from selected crystals in distinct orientations enhances completeness. Application of the two approaches enables high-quality structural models to be obtained from limited or sensitive samples.

YouTube video by International Union of Crystallography

Regulation of pharmaceutical lead compounds does not yet involve the need for patient-relevant macromolecular structures determined at 37°C; nevertheless, we highlight the advantages of these to ensure chemical relevance and the identification of changes which may occur under physiological conditions. The PDB and CSD entries for diffraction data measured at ≥37°C, as well as a cryoEM 37°C freeze-trapped structure, are considered and the description includes structures obtained well above 37°C that are relevant to thermophile and hyperthermophile structural biology.

In situ cryo-EM coupled with 2D template matching (2DTM) holds the potential to visualize the cellular structureome in context, but further developments are required to make this a reality. We describe Leopard-EM (Location and orientation of particles found using two-dimensional tEmplate Matching), an extensible Python package for 2DTM to accelerate in situ structural biology.

In situ cryo-EM coupled with 2D template matching (2DTM) holds the potential to visualize the cellular structureome in context, but further developments are required to make this a reality. We describ...

We present the crystal structure of a segment of human ADAMTS-5 that includes part of the cysteine-rich domain and the spacer domain.

This study presents the first crystal structure of a hydroxycinnamoyl-CoA:tartaric acid hydroxycinnamoyl transferase from E. purpurea (EpHTT), a specialized BAHD acyltransferase central to the biosynthesis of chicoric acid. We successfully expressed, purified and crystallized the full-length EpHTT protein (residues 1–424) and determined its high-resolution structure at 2.38 Å.

This article reviews the growth over the last ten years of the CCP-EM Spring Symposium. The conference is now established as a popular meeting for practitioners of life-science cryoEM and highlights new methods and exemplar applications.

Xtricorder is a tool for analysing crystallographic data, featuring a likelihood-enhanced self-rotation function and a novel `composite-section diagram' thereof, which aids interpretation and enables a machine learning-enhanced prediction of asymmetric unit content.

We present CryoLike, a user-friendly Python workflow for calculating the image-to-structure likelihood in cryo-electron microscopy.

A surface-entropy reduction approach for human Polo-like kinase 1 (PLK1) enabled a reproducible and robust crystallization system for this cancer drug target. Co-crystal structures with representatives of three different small-molecule inhibitor classes were elucidated. For two of them, co-crystal structures with PLK1 were also determined via a previously published crystallization approach using a designed ankyrin-repeat protein (DARPin), and the two approaches are compared.

Registration fee to attend the Structural Biology Meeting 2025, to be held at the Dorothy Crowfoot Hodgkin Building in Oxford, on Friday 12th December 2025

The effect of radiation damage in MicroED has been studied. By only including data collected at low accumulated electron dose during data processing, the effects of radiation damage can be reduced.