Addgene
@addgene.bsky.social
Sharing speeds science! Addgene helps scientists around the world find and share research materials - plasmids, viral preps, antibodies, data, and more.
🤩 We love seeing agar stabs arrive at the lab! Thanks for the explainer and great tips @bumblingbiochemist.bsky.social
That plasmid you order for your lab will likely come as an agar stab...
youtube.com/shorts/QVMde... bit.ly/plasmid_prep...
youtube.com/shorts/QVMde... bit.ly/plasmid_prep...
That plasmid you order for your lab will likely come as an agar stab...
YouTube video by the bumbling biochemist
youtube.com
December 12, 2025 at 3:21 PM
🤩 We love seeing agar stabs arrive at the lab! Thanks for the explainer and great tips @bumblingbiochemist.bsky.social
pBIG1a-PAF1C(CTR9/CDC73/PAF1/LEO1/WDR61)
Depositor: Benjamin Blencowe
Purpose: Protein expression in insect cells
https://twp.ai/4isUN9
Depositor: Benjamin Blencowe
Purpose: Protein expression in insect cells
https://twp.ai/4isUN9
December 10, 2025 at 5:01 PM
pBIG1a-PAF1C(CTR9/CDC73/PAF1/LEO1/WDR61)
Depositor: Benjamin Blencowe
Purpose: Protein expression in insect cells
https://twp.ai/4isUN9
Depositor: Benjamin Blencowe
Purpose: Protein expression in insect cells
https://twp.ai/4isUN9
pAAV_BiPVe4_eGFP
Depositor: Gordon Fishell
Expressing eGFP driven by chandelier cell-targeting enhancer
In-stock AAV preps available in AAV1 and PHPeB; other serotypes available on demand.
https://twp.ai/4isUN5
Depositor: Gordon Fishell
Expressing eGFP driven by chandelier cell-targeting enhancer
In-stock AAV preps available in AAV1 and PHPeB; other serotypes available on demand.
https://twp.ai/4isUN5
December 8, 2025 at 9:00 PM
pAAV_BiPVe4_eGFP
Depositor: Gordon Fishell
Expressing eGFP driven by chandelier cell-targeting enhancer
In-stock AAV preps available in AAV1 and PHPeB; other serotypes available on demand.
https://twp.ai/4isUN5
Depositor: Gordon Fishell
Expressing eGFP driven by chandelier cell-targeting enhancer
In-stock AAV preps available in AAV1 and PHPeB; other serotypes available on demand.
https://twp.ai/4isUN5
We always love new plasmids coming online! 💖 Thanks for sharing @schmittlab-ucla.bsky.social
Plasmids for PINK1-SPARK are now available on Addgene! Find them here: www.addgene.org/browse/artic...
December 8, 2025 at 7:39 PM
We always love new plasmids coming online! 💖 Thanks for sharing @schmittlab-ucla.bsky.social
🧠🧬 CRISPR editing in postmitotic cells like neurons is hard. But the Fellman, Conklin, @kampmann.bsky.social, @doudna-lab.bsky.social labs and colleagues @innovativegenomics.bsky.social have found strategies to make it work! Find plasmids here: https://twp.ai/4isUMv
December 8, 2025 at 3:00 PM
🧠🧬 CRISPR editing in postmitotic cells like neurons is hard. But the Fellman, Conklin, @kampmann.bsky.social, @doudna-lab.bsky.social labs and colleagues @innovativegenomics.bsky.social have found strategies to make it work! Find plasmids here: https://twp.ai/4isUMv
Reposted by Addgene
Congratulations to @addgene.bsky.social depositors @jourdainlab.bsky.social, @mpercharde.bsky.social, and @alejofraticelli.bsky.social (and maybe some future Addgene depositors?) on this honor!
www.addgene.org/Alexis_Jourd...
www.addgene.org/Michelle_Per...
www.addgene.org/Alejo_Rodrig...
www.addgene.org/Alexis_Jourd...
www.addgene.org/Michelle_Per...
www.addgene.org/Alejo_Rodrig...
December 4, 2025 at 2:21 PM
Congratulations to @addgene.bsky.social depositors @jourdainlab.bsky.social, @mpercharde.bsky.social, and @alejofraticelli.bsky.social (and maybe some future Addgene depositors?) on this honor!
www.addgene.org/Alexis_Jourd...
www.addgene.org/Michelle_Per...
www.addgene.org/Alejo_Rodrig...
www.addgene.org/Alexis_Jourd...
www.addgene.org/Michelle_Per...
www.addgene.org/Alejo_Rodrig...
Congrats! Thanks for sharing your tools with so many scientists around the world. Sharing speeds science!
@addgene.bsky.social We odeposit plasmids even when we don’t publish the work. After 40 years in this field, I still believe that,despite competition and personal ambitions,science is one of the most collaborative and remarkable enterprises humans have created. It is satisfying to see this milestone
December 3, 2025 at 7:28 PM
Congrats! Thanks for sharing your tools with so many scientists around the world. Sharing speeds science!
AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA
Depositor: Allen Institute for Brain Science, Jonathan Ting
Nuclear SYFP2 labeling in neurons, for nuclear isolation and scRNA-seq applications
In-stock AAV preps available in PHPeB; other serotypes available on demand.
https://twp.ai/4isPbK
Depositor: Allen Institute for Brain Science, Jonathan Ting
Nuclear SYFP2 labeling in neurons, for nuclear isolation and scRNA-seq applications
In-stock AAV preps available in PHPeB; other serotypes available on demand.
https://twp.ai/4isPbK
December 1, 2025 at 9:00 PM
AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA
Depositor: Allen Institute for Brain Science, Jonathan Ting
Nuclear SYFP2 labeling in neurons, for nuclear isolation and scRNA-seq applications
In-stock AAV preps available in PHPeB; other serotypes available on demand.
https://twp.ai/4isPbK
Depositor: Allen Institute for Brain Science, Jonathan Ting
Nuclear SYFP2 labeling in neurons, for nuclear isolation and scRNA-seq applications
In-stock AAV preps available in PHPeB; other serotypes available on demand.
https://twp.ai/4isPbK
New at Addgene! Photoswitchable HaloTag, enabling robust and selective labeling of HaloTag on the surface of living cells using visible-light activation. From the @clairedeo.bsky.social lab.
Walterspiel et al. Angew. Chem. Int. Ed. 2025
https://twp.ai/4isOad
Walterspiel et al. Angew. Chem. Int. Ed. 2025
https://twp.ai/4isOad
December 1, 2025 at 6:01 PM
New at Addgene! Photoswitchable HaloTag, enabling robust and selective labeling of HaloTag on the surface of living cells using visible-light activation. From the @clairedeo.bsky.social lab.
Walterspiel et al. Angew. Chem. Int. Ed. 2025
https://twp.ai/4isOad
Walterspiel et al. Angew. Chem. Int. Ed. 2025
https://twp.ai/4isOad
Reposted by Addgene
Happy #FluorescenceFriday! Our #CellLineOfTheWeek is PCNA - illuminating a protein involved in DNA replication and repair!
Our cell lines & plasmids are available at cost to support discovery: https://www.allencell.org/cell-catalog.html
📦 @coriellinstitute.bsky.social and @addgene.bsky.social
Our cell lines & plasmids are available at cost to support discovery: https://www.allencell.org/cell-catalog.html
📦 @coriellinstitute.bsky.social and @addgene.bsky.social
November 28, 2025 at 4:19 PM
Happy #FluorescenceFriday! Our #CellLineOfTheWeek is PCNA - illuminating a protein involved in DNA replication and repair!
Our cell lines & plasmids are available at cost to support discovery: https://www.allencell.org/cell-catalog.html
📦 @coriellinstitute.bsky.social and @addgene.bsky.social
Our cell lines & plasmids are available at cost to support discovery: https://www.allencell.org/cell-catalog.html
📦 @coriellinstitute.bsky.social and @addgene.bsky.social
Thank you to the 6,700 researchers who deposit their plasmids with us — your contributions help build a more connected scientific community & unlock the full potential of research!
Addgene will be closed Thurs., Nov. 27 & Fri., Nov. 28 for the Thanksgiving holiday.
Addgene will be closed Thurs., Nov. 27 & Fri., Nov. 28 for the Thanksgiving holiday.
November 26, 2025 at 3:00 PM
Thank you to the 6,700 researchers who deposit their plasmids with us — your contributions help build a more connected scientific community & unlock the full potential of research!
Addgene will be closed Thurs., Nov. 27 & Fri., Nov. 28 for the Thanksgiving holiday.
Addgene will be closed Thurs., Nov. 27 & Fri., Nov. 28 for the Thanksgiving holiday.
pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH
Depositor: Ivo Spiegel
Kir2.1 expression for silencing neurons and mScarlet-KASH expression
In-stock AAV preps available in AAV8; other serotypes available on demand.
https://twp.ai/4itAvh
Depositor: Ivo Spiegel
Kir2.1 expression for silencing neurons and mScarlet-KASH expression
In-stock AAV preps available in AAV8; other serotypes available on demand.
https://twp.ai/4itAvh
November 24, 2025 at 9:00 PM
pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH
Depositor: Ivo Spiegel
Kir2.1 expression for silencing neurons and mScarlet-KASH expression
In-stock AAV preps available in AAV8; other serotypes available on demand.
https://twp.ai/4itAvh
Depositor: Ivo Spiegel
Kir2.1 expression for silencing neurons and mScarlet-KASH expression
In-stock AAV preps available in AAV8; other serotypes available on demand.
https://twp.ai/4itAvh
New at Addgene! MitoTRACER, a cre-lox based reporter of cell-to-cell mitochondrial transfer. Used to study transfer from nerve to cancer cells and its impact on metabolism and metastasis.
By @greletlab.bsky.social
🔗 https://twp.ai/4itAyW
By @greletlab.bsky.social
🔗 https://twp.ai/4itAyW
November 24, 2025 at 4:01 PM
New at Addgene! MitoTRACER, a cre-lox based reporter of cell-to-cell mitochondrial transfer. Used to study transfer from nerve to cancer cells and its impact on metabolism and metastasis.
By @greletlab.bsky.social
🔗 https://twp.ai/4itAyW
By @greletlab.bsky.social
🔗 https://twp.ai/4itAyW
Reposted by Addgene
Happy #FlourescenceFriday! Our #CellLineOfTheWeek is SON, a cell line and plasmid that illuminates ✨ nuclear speckles ✨ a real science word for nuclear proteins involved in gene expression.
📦 @coriellinstitute.bsky.social and @addgene.bsky.social
📦 @coriellinstitute.bsky.social and @addgene.bsky.social
November 22, 2025 at 12:22 AM
Happy #FlourescenceFriday! Our #CellLineOfTheWeek is SON, a cell line and plasmid that illuminates ✨ nuclear speckles ✨ a real science word for nuclear proteins involved in gene expression.
📦 @coriellinstitute.bsky.social and @addgene.bsky.social
📦 @coriellinstitute.bsky.social and @addgene.bsky.social
Anti-Kvbeta2/KCNAB2 K+ channel [K17/70R]
Depositor: James Trimmer
Purpose: Anti-Kvbeta2/KCNAB2 K+ channel (Mouse) recombinant mouse monoclonal antibody
https://twp.ai/4it5U8
Depositor: James Trimmer
Purpose: Anti-Kvbeta2/KCNAB2 K+ channel (Mouse) recombinant mouse monoclonal antibody
https://twp.ai/4it5U8
November 21, 2025 at 7:00 PM
Anti-Kvbeta2/KCNAB2 K+ channel [K17/70R]
Depositor: James Trimmer
Purpose: Anti-Kvbeta2/KCNAB2 K+ channel (Mouse) recombinant mouse monoclonal antibody
https://twp.ai/4it5U8
Depositor: James Trimmer
Purpose: Anti-Kvbeta2/KCNAB2 K+ channel (Mouse) recombinant mouse monoclonal antibody
https://twp.ai/4it5U8
Brie and Brunello, Calabrese and Dolcetto, Julianna and Jacquere! 🧀🍷 Are we hosting a wine tasting? Not exactly… These are all pooled plasmid libraries for genome-wide screening!
November 20, 2025 at 5:00 PM
Brie and Brunello, Calabrese and Dolcetto, Julianna and Jacquere! 🧀🍷 Are we hosting a wine tasting? Not exactly… These are all pooled plasmid libraries for genome-wide screening!
5'-3xFLAG Msl1-exon minimal CMV pCDNA5
Depositor: Benjamin Blencowe
Purpose: LR vector for integration of Msl1-exon into N2a FRT rtTA3 expression cells
https://twp.ai/4it5R3
Depositor: Benjamin Blencowe
Purpose: LR vector for integration of Msl1-exon into N2a FRT rtTA3 expression cells
https://twp.ai/4it5R3
November 19, 2025 at 5:00 PM
5'-3xFLAG Msl1-exon minimal CMV pCDNA5
Depositor: Benjamin Blencowe
Purpose: LR vector for integration of Msl1-exon into N2a FRT rtTA3 expression cells
https://twp.ai/4it5R3
Depositor: Benjamin Blencowe
Purpose: LR vector for integration of Msl1-exon into N2a FRT rtTA3 expression cells
https://twp.ai/4it5R3
Who doesn't love a library? A pooled library, that is! Grab a hot drink and your reading glasses and enjoy browsing the libraries Addgene has to offer:
https://twp.ai/4it3AO
https://twp.ai/4it3AO
November 19, 2025 at 3:00 PM
Who doesn't love a library? A pooled library, that is! Grab a hot drink and your reading glasses and enjoy browsing the libraries Addgene has to offer:
https://twp.ai/4it3AO
https://twp.ai/4it3AO
Addgene's site is functional once again! 👩💻 We will provide an update if we identify any other issues.
addgenestatus.org
addgenestatus.org
November 18, 2025 at 3:23 PM
Addgene's site is functional once again! 👩💻 We will provide an update if we identify any other issues.
addgenestatus.org
addgenestatus.org
Addgene's site is currently unavailable due to the issues with Cloudfare's cybersecurity service. You can check the status of our site as Cloudfare works to resolve these issues:
addgenestatus.org
addgenestatus.org
November 18, 2025 at 2:01 PM
Addgene's site is currently unavailable due to the issues with Cloudfare's cybersecurity service. You can check the status of our site as Cloudfare works to resolve these issues:
addgenestatus.org
addgenestatus.org
pAAV_BiVIPe4_ChR2_mCherry
Depositor: Gordon Fishell
Expresses mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer.
In-stock AAV preps available in AAV1 and PHPeB; other serotypes available on demand.
https://twp.ai/4it7qP
Depositor: Gordon Fishell
Expresses mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer.
In-stock AAV preps available in AAV1 and PHPeB; other serotypes available on demand.
https://twp.ai/4it7qP
November 17, 2025 at 9:00 PM
pAAV_BiVIPe4_ChR2_mCherry
Depositor: Gordon Fishell
Expresses mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer.
In-stock AAV preps available in AAV1 and PHPeB; other serotypes available on demand.
https://twp.ai/4it7qP
Depositor: Gordon Fishell
Expresses mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer.
In-stock AAV preps available in AAV1 and PHPeB; other serotypes available on demand.
https://twp.ai/4it7qP
Happening soon at #SfN25! 🧠
Come chat about antibodies with @ipiproteins.bsky.social during IPI office hours — booth #3625, 11–3.
Let’s talk axon guidance, the synaptic cleft, and the next antibodies you want to see from Addgene × IPI.
Come chat about antibodies with @ipiproteins.bsky.social during IPI office hours — booth #3625, 11–3.
Let’s talk axon guidance, the synaptic cleft, and the next antibodies you want to see from Addgene × IPI.
November 17, 2025 at 4:00 PM
Happening soon at #SfN25! 🧠
Come chat about antibodies with @ipiproteins.bsky.social during IPI office hours — booth #3625, 11–3.
Let’s talk axon guidance, the synaptic cleft, and the next antibodies you want to see from Addgene × IPI.
Come chat about antibodies with @ipiproteins.bsky.social during IPI office hours — booth #3625, 11–3.
Let’s talk axon guidance, the synaptic cleft, and the next antibodies you want to see from Addgene × IPI.
Heading to #SfN25? Look for some of the labs and hot #neuroskyence tools featured in our latest blog post!
Fresh batch of Hot Plasmids here! Find new tools for:
🧠 Neural cell-surface proteomics
🔋 Nerve-to-cancer mitochondrial transfer @greletlab.bsky.social
🔬 Red calcium biosensors @massecklab.bsky.social & @piatkevich.bsky.social
✂️ CRISPRa-AAV for adipocytes @nadavahituv.bsky.social
& more!
🧠 Neural cell-surface proteomics
🔋 Nerve-to-cancer mitochondrial transfer @greletlab.bsky.social
🔬 Red calcium biosensors @massecklab.bsky.social & @piatkevich.bsky.social
✂️ CRISPRa-AAV for adipocytes @nadavahituv.bsky.social
& more!
November 16, 2025 at 1:58 PM
Heading to #SfN25? Look for some of the labs and hot #neuroskyence tools featured in our latest blog post!
MAGIC Toolbox: MultiSite Assembly of Gateway Induced Clones ✨🧬 Thanks for sharing @wythelab.bsky.social!
Find the plasmids here: www.addgene.org/browse/artic...
Find the plasmids here: www.addgene.org/browse/artic...
November 14, 2025 at 7:28 PM
MAGIC Toolbox: MultiSite Assembly of Gateway Induced Clones ✨🧬 Thanks for sharing @wythelab.bsky.social!
Find the plasmids here: www.addgene.org/browse/artic...
Find the plasmids here: www.addgene.org/browse/artic...