Lightnews — Scholar-powered news

Addgene @addgene.bsky.social · 8h

pBiT3.1-N_DDB1:1-1140
Depositor: Cheryl Arrowsmith
Purpose: Mammalian expression of full-length human DDB1. N-terminal HiBiT tag.

https://twp.ai/4is1ck

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Addgene @addgene.bsky.social · 11h

Find these amino acid reporters here! 🧬🧪https://www.addgene.org/browse/article/28244242/

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Addgene @addgene.bsky.social · 13h

Chonnettia Jones, Addgene’s President & Executive Director, is one of Endpoints News’ 2025 Top Women in Biopharma R&D! She is driving Addgene’s growth to enable more discovery and innovation, powered by the sharing of scientific resources.
🔗

endpoints.news

Meet 20 women reshaping biopharma, from early discovery to patient advocacy

twp.ai

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Reposted by Addgene

Elinne Becket, PhD @bielleogy.bsky.social · 1d

Doing Western Blots in lab classes today. Send me your "best" (aka worst) WBs for me to show my students how not easy they are 😆

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Addgene @addgene.bsky.social · 1d

Choosing the right dye or ligand can make or break an experiment. 💡
This guide from @promegacorporation.bsky.social breaks down the strengths and tradeoffs — helping you make the best match for your assay.
🔗 Read the blog:

blog.addgene.org

Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

twp.ai

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Addgene @addgene.bsky.social · 2d

pGP-AAV-syn-flex-iGABASnFR2(no bind)-WPRE
Depositor: GENIE Project
Syn-driven, Cre-dependent expression of the improved GABA sensor control iGABASnFR2(no bind) (no change in fluorescence).
In-stock AAV preps available in AAV1 and AAV5; other serotypes available on demand.
https://twp.ai/4irnYg

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Reposted by Addgene

Allen Institute @alleninstitute.org · 5d

Happy #FluorescenceFriday! Our #CellLineOfTheWeek is SMC (structural maintenance of chromosomes) protein 1A! 🧬🛠️

Our cell lines & plasmids are available for just the cost of shipping to reduce the barriers to scientific discovery.

📦 Dist. by @coriellinstitute.bsky.social & @addgene.bsky.social

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Addgene @addgene.bsky.social · 2d

New CRISPR libraries from the @johndoench.bsky.social and Root labs — Jacquere (human) and Julianna (mouse).
A perfect pairing for your next screen. 🍷🧀
https://twp.ai/9PX2kT
https://twp.ai/4irnYP

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Addgene @addgene.bsky.social · 3d

Thanks for sharing the plasmids at Addgene! www.addgene.org/browse/artic...

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Addgene @addgene.bsky.social · 6d

HiBiT takes split-luciferase assays to a whole new, highly-sensitive level! 💡 Read about the many applications enabled by HiBiT in this guest blog from our friends at @promegacorporation.bsky.social

blog.addgene.org

Exploring Applications of the Bioluminescent HiBiT Tag

twp.ai

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Addgene @addgene.bsky.social · 6d

Andrew Willoughby from the Nimchuk Lab at UNC wrote this great intro to RUBY for the Addgene blog! blog.addgene.org/ruby-red-sil...

RUBY-Red Siliques

RUBY-red is a new plant marker that uses a bright red color for easy identification of transgenic plants.

blog.addgene.org

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Addgene @addgene.bsky.social · 7d

New AAV tools from @e11bio.bsky.social! PRISM uses protein-tag barcodes for high-throughput reconstruction of neurons and other cell-labeling applications. https://twp.ai/4iruEB

A 3D rendering of a network of neurons from a rectangular volume. Each neuron is filled with a slightly different color, making each cell body and its projections distinct from its neighbors.

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Addgene @addgene.bsky.social · 8d

We've just added 250+ more plasmids to the Promega Collection
• Human ORFs with tags like NanoLuc®, HaloTag®, SmBiT, and LgBiT
• NanoBiT® and NanoBRET® plasmids for live-cell assays
• Reporter vectors for gene expression and pathway analysis
https://twp.ai/4iriLO

Accelerate your research, no MTA required. Blugene mascot with a lightbulb, and Addgene and Promega logos.

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Addgene @addgene.bsky.social · 9d

AiP13987: pAAV-AiE0452h_3xC2-minBG-jRGECO1a-WPRE3-BGHpA
Depositor: AIBS, Jonathan Ting
Expression of jRGECO1a in striatal indirect pathway medium spiny neurons.
In-stock AAV preps available in PHPeB; other serotypes available on demand.
https://twp.ai/4iriLD

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Addgene @addgene.bsky.social · 9d

Newly released plasmids from @vikpc.bsky.social, Phil Sharp, and Robert Langer: next-generation prime editors (vPE) with strikingly low error rates.
https://twp.ai/4iriKs

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Addgene @addgene.bsky.social · 12d

Anti-FTH1 [GP5]
Depositor: Melina Fan
Anti-Ferritin heavy chain 1 (Human) recombinant mouse monoclonal antibody

https://twp.ai/4iqKGE

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Addgene @addgene.bsky.social · 12d

Baby KJ's metabolic disorder was treated through an intense six-month effort to create a personalized CRISPR base editor — made possible by the preceding nine years of base editing research. Learn about the advances that went into this year's major milestone.

blog.addgene.org

The Advances Behind The World's First Personalized CRISPR Treatment

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Addgene @addgene.bsky.social · 13d

Protein labeling made simple.
Self-labeling tags like HaloTag® let you visualize proteins in real time with the dye or ligand of your choice. Find out how these systems expand your experimental toolkit.
🔗 Read the blog:

blog.addgene.org

Live and Let Dye: Self-Labeling Protein Tags

twp.ai

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Addgene @addgene.bsky.social · 14d

LjUBQ:MtN26_:T35s
Depositor: Wolf Scheible
Purpose: Overexpression of Peptide coding gene (Binary vector)

https://twp.ai/4iqKDp

Addgene @addgene.bsky.social · 15d

Visit our IPI antibody collection to find IPI's epitope tag antibodies and many more!
www.addgene.org/antibodies/p...

🔬 Explore real application data from actual experiments with every antibody.

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Addgene @addgene.bsky.social · 15d

Cool science alert! A plasmid encoding a photodegradable hydrogel-forming protein called PhoCoil. Find it here:
https://twp.ai/4iqKMK
@coledeforest.bsky.social

Addgene: PhoCoil

Plasmid PhoCoil from Dr. Cole DeForest's lab contains the insert PhoCoil and is published in Sci Adv. 2025 Aug 29;11(35):eadx3472. doi: 10.1126/sciadv.adx3472. Epub 2025 Aug 27. This plasmid is available through Addgene.

twp.ai

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Reposted by Addgene

abberior @abberior.rocks · 19d

3 color confocal image of a #Drosophila 🪰embryo stained for chitin (🟦STAR RED), tubulin (🟥STAR ORANGE), and DNA (🟩PicoGreen)

#fluorescencefriday

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Reposted by Addgene

abberior @abberior.rocks · 15d

A plasmid is available from Addgene (www.addgene.org/104586/) to create the protein which can then be conjugated with a SNAP-ligand of choice (see link.abberior.rocks/xgtw9).

Addgene: pYZ205

Plasmid pYZ205 from Dr. Yinhua Zhang's lab contains the insert SNAP-6His-CBD and is published in Unpublished This plasmid is available through Addgene.

www.addgene.org

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Addgene @addgene.bsky.social · 15d

Want to control when and how a protein is degraded?
Degron tags are powerful tools for probing protein stability and function. Learn how they work — and how they can accelerate your research.
🔗 Read the blog:

blog.addgene.org

Plasmids 101: Degron Tags

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Addgene @addgene.bsky.social · 15d

Tags, reporters, luciferase — oh my!
To celebrate the expansion of the Promega Plasmid Collection, we’re spotlighting some of our most popular blogs on the technologies researchers rely on every day — tools you can access through Addgene.

Learn the Science Behind the Tools, with Addgene and Promega logos and the Blugene mascot holding a light bulb

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