Cell Biology J-Club
@cellclub.bsky.social
RanBP2-dependent annulate lamellae drive nuclear pore assembly and nuclear expansion www.biorxiv.org/content/10.1...
RanBP2-dependent annulate lamellae drive nuclear pore assembly and nuclear expansion
Nuclear pore complexes (NPCs) enable nucleocytoplasmic transport. While NPCs primarily localize to the nuclear envelope (NE), they also appear in cytoplasmic endoplasmic reticulum (ER) membranes calle...
www.biorxiv.org
November 26, 2025 at 3:20 PM
RanBP2-dependent annulate lamellae drive nuclear pore assembly and nuclear expansion www.biorxiv.org/content/10.1...
Cryo-ET and MD simulations reveal that dynein-2 is tuned for binding to the A-tubule of the ciliary doublet | The EMBO Journal www.embopress.org/doi/full/10....
Cryo-ET and MD simulations reveal that dynein-2 is tuned for binding to the A-tubule of the ciliary doublet | The EMBO Journal
imageimageDynein-2 is a motor protein essential for retrograde intraflagellar transport (IFT)
on the A-tubule of ciliary doublet microtubules. Here, biochemical and cryo-ET analyses
demonstrate that d...
www.embopress.org
November 26, 2025 at 3:17 PM
Cryo-ET and MD simulations reveal that dynein-2 is tuned for binding to the A-tubule of the ciliary doublet | The EMBO Journal www.embopress.org/doi/full/10....
Adherent cells sustain membrane tension gradients independently of migration
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Adherent cells sustain membrane tension gradients independently of migration - Nature Communications
This study shows that adherent cells maintain membrane tension gradients even without moving. Using a fluorescent probe, the authors reveal that actin and adhesion forces shape these gradients, provid...
www.nature.com
November 26, 2025 at 3:16 PM
Adherent cells sustain membrane tension gradients independently of migration
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Sec14L6 is a phosphoinositide transporter that regulates phosphoinositide homeostasis and biogenesis of lipid droplets
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Sec14L6 is a phosphoinositide transporter that regulates phosphoinositide homeostasis and biogenesis of lipid droplets - Nature Communications
This study identifies Sec14L6 as a crucial lipid transporter that balances phosphoinositide levels between the endoplasmic reticulum and lipid droplets, thereby promoting the formation of lipid drople...
www.nature.com
November 26, 2025 at 3:15 PM
Sec14L6 is a phosphoinositide transporter that regulates phosphoinositide homeostasis and biogenesis of lipid droplets
www.nature.com/articles/s41...
www.nature.com/articles/s41...
The neuropathy-linked protein TECPR2 is a Rab5 effector that regulates cargo recycling from early endosomes
www.nature.com/articles/s41...
www.nature.com/articles/s41...
The neuropathy-linked protein TECPR2 is a Rab5 effector that regulates cargo recycling from early endosomes - Nature Communications
Loss-of-function mutations in TECPR2 result in a form of hereditary sensory and autonomic neuropathy. Here, the authors report that TECPR2 is a Rab5 effector that mediates actin-dependent endocytic ca...
www.nature.com
November 26, 2025 at 3:13 PM
The neuropathy-linked protein TECPR2 is a Rab5 effector that regulates cargo recycling from early endosomes
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Homocysteine disrupts lysosomal function by V-ATPase inhibition url: rupress.org/jcb/article-...
Homocysteine disrupts lysosomal function by V-ATPase inhibition
Yang et al. reports that homocysteine, an intermediate in methionine-cysteine metabolism and the cause of the life-threatening disease homocystinuria, disr
rupress.org
November 25, 2025 at 2:55 PM
Homocysteine disrupts lysosomal function by V-ATPase inhibition url: rupress.org/jcb/article-...
Phosphorylation-coupled autoregulation of TANGO1 and Sec16A maintains functional ER exit sites
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Phosphorylation-coupled autoregulation of TANGO1 and Sec16A maintains functional ER exit sites - Nature Communications
The ER exit site is a portal on the endoplasmic reticulum where secretory proteins depart. Here, the authors revealed that a balanced phosphorylation state of the ER exit site proteins TANGO1 and Sec1...
www.nature.com
November 25, 2025 at 2:54 PM
Phosphorylation-coupled autoregulation of TANGO1 and Sec16A maintains functional ER exit sites
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Optogenetic control of mechanotransduction based on light-induced homodimerization of talin url: journals.biologists.com/jcs/article-...
Optogenetic control of mechanotransduction based on light-induced homodimerization of talin
Integrin-based adhesion complexes serve as primary sites for actomyosin force transmission to the extracellular matrix, providing traction that drives cell mechanical responses including adhesion, mig...
journals.biologists.com
November 24, 2025 at 4:49 PM
Optogenetic control of mechanotransduction based on light-induced homodimerization of talin url: journals.biologists.com/jcs/article-...
Apical and basolateral plasma membranes in epithelial cells have distinct lipidomes and biophysical properties www.biorxiv.org/content/10.1...
Apical and basolateral plasma membranes in epithelial cells have distinct lipidomes and biophysical properties
Epithelial cell polarization is essential for many physiological processes, including tissue morphogenesis, nutrient absorption, barrier integrity, and directional secretion. A defining feature of suc...
www.biorxiv.org
November 24, 2025 at 4:45 PM
Apical and basolateral plasma membranes in epithelial cells have distinct lipidomes and biophysical properties www.biorxiv.org/content/10.1...
A partner switch from CDS2 to RAB1A redirects MBOAT7 to inhibit DGAT2-mediated lipid droplet growth www.biorxiv.org/content/10.1...
www.biorxiv.org
November 24, 2025 at 4:43 PM
A partner switch from CDS2 to RAB1A redirects MBOAT7 to inhibit DGAT2-mediated lipid droplet growth www.biorxiv.org/content/10.1...
In @elife: Human dynein-dynactin is a fast processive motor in living cells doi.org/10.7554/eLif...
Human dynein-dynactin is a fast processive motor in living cells
doi.org
November 24, 2025 at 4:33 PM
In @elife: Human dynein-dynactin is a fast processive motor in living cells doi.org/10.7554/eLif...
Polyamines sustain epithelial regeneration in aged intestines by modulating protein homeostasis
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Polyamines sustain epithelial regeneration in aged intestines by modulating protein homeostasis - Nature Cell Biology
Minetti, Omrani et al. report that delayed intestinal regeneration results from protein homeostasis stress and can be improved by modulation of the polyamine pathway dynamics.
www.nature.com
November 24, 2025 at 4:31 PM
Polyamines sustain epithelial regeneration in aged intestines by modulating protein homeostasis
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Super-resolved imaging and Bayesian analysis of single exocytosis events reveals molecular-scale patterning by cortical microtubule arrays www.biorxiv.org/content/10.1...
Super-resolved imaging and Bayesian analysis of single exocytosis events reveals molecular-scale patterning by cortical microtubule arrays
The microtubule cytoskeleton organizes exocytosis to enable cellular morphogenesis, but how non-centrosomal arrays control exocytotic site positioning remains poorly understood. Using elongating plant...
www.biorxiv.org
November 21, 2025 at 4:15 PM
Super-resolved imaging and Bayesian analysis of single exocytosis events reveals molecular-scale patterning by cortical microtubule arrays www.biorxiv.org/content/10.1...
A microscopy-based CRISPR screening platform enables organellar functional genomics and illuminates ciliary biology: Developmental Cell www.cell.com/developmenta...
A microscopy-based CRISPR screening platform enables organellar functional genomics and illuminates ciliary biology
Sun et al. develop a microscopy-based CRISPR screening platform that combines ease
of implementation with flexible analysis of live-cell or antibody-based markers. Screens
in human cells using a serie...
www.cell.com
November 21, 2025 at 3:40 PM
A microscopy-based CRISPR screening platform enables organellar functional genomics and illuminates ciliary biology: Developmental Cell www.cell.com/developmenta...
Inhibition of oligomeric BAX by an anti-apoptotic dimer: Cell www.cell.com/cell/fulltex...
Inhibition of oligomeric BAX by an anti-apoptotic dimer
A full-length dimeric form of the anti-apoptotic protein BCL-w can block oligomeric
BAX-driven mitochondrial permeabilization and apoptosis, revealing a new layer of
cell-death control.
www.cell.com
November 21, 2025 at 3:38 PM
Inhibition of oligomeric BAX by an anti-apoptotic dimer: Cell www.cell.com/cell/fulltex...
A unified mechanism for mitochondrial damage sensing in PINK1-Parkin–mediated mitophagy | The EMBO Journal www.embopress.org/doi/full/10....
A unified mechanism for mitochondrial damage sensing in PINK1-Parkin–mediated mitophagy | The EMBO Journal
imageimageDamaged mitochondria can be eliminated from the cell by the PINK1-Parkin mitophagy
pathway, but whether there is a convergent trigger for mitophagy has been unclear.
This report shows that d...
www.embopress.org
November 21, 2025 at 3:36 PM
A unified mechanism for mitochondrial damage sensing in PINK1-Parkin–mediated mitophagy | The EMBO Journal www.embopress.org/doi/full/10....
Talin–tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation url: rupress.org/jcb/article/...
Talin–tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation | Journal of Cell Biology | Rockefeller University Press
Li et al. show that multidomain interactions between talin and tensin3 regulate integrin activity, fibrillar adhesion formation, fibronectin assembly, and
rupress.org
November 21, 2025 at 3:35 PM
Talin–tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation url: rupress.org/jcb/article/...
ATP-dependent actin barbed-end fluctuations at steady state | PNAS www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
November 20, 2025 at 6:49 PM
ATP-dependent actin barbed-end fluctuations at steady state | PNAS www.pnas.org/doi/10.1073/...
Joubert syndrome 26 protein enforces compartmentalized motility of a ciliary kinesin | PNAS www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
November 20, 2025 at 6:48 PM
Joubert syndrome 26 protein enforces compartmentalized motility of a ciliary kinesin | PNAS www.pnas.org/doi/10.1073/...
The nonfibrillar multiplexin collagen CLE-1 defines cholinergic synapse identity | Science Advances www.science.org/doi/10.1126/...
The nonfibrillar multiplexin collagen CLE-1 defines cholinergic synapse identity
A nonfibrillar collagen organizes cholinergic synapses by guiding receptor localization and ensuring synaptic identity.
www.science.org
November 20, 2025 at 1:50 PM
The nonfibrillar multiplexin collagen CLE-1 defines cholinergic synapse identity | Science Advances www.science.org/doi/10.1126/...
Structural basis of regulated N-glycosylation at the secretory translocon
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structural basis of regulated N-glycosylation at the secretory translocon - Nature
The structural mechanism of a natively isolated GRP94 folding intermediate tethered to a CCDC134-bound translocon is described, revealing how the nascent chain remodels the translocon to control N-gly...
www.nature.com
November 19, 2025 at 5:16 PM
Structural basis of regulated N-glycosylation at the secretory translocon
www.nature.com/articles/s41...
www.nature.com/articles/s41...
The lactate sensor NDRG3 decelerates ER-to-Golgi transport through interaction with the long isoform of syntaxin-5 | PNAS www.pnas.org/doi/10.1073/...
The lactate sensor NDRG3 decelerates ER-to-Golgi transport through interaction with the long isoform of syntaxin-5 | PNAS
BET1, GOSR2, and STX5 variants can cause fatal inherited diseases, including epilepsies,
muscular dystrophy, and multisystemic disorders. Together ...
www.pnas.org
November 19, 2025 at 3:09 PM
The lactate sensor NDRG3 decelerates ER-to-Golgi transport through interaction with the long isoform of syntaxin-5 | PNAS www.pnas.org/doi/10.1073/...
High-accuracy protein complex structure modeling based on sequence-derived structure complementarity
www.nature.com/articles/s41...
www.nature.com/articles/s41...
High-accuracy protein complex structure modeling based on sequence-derived structure complementarity - Nature Communications
In this work the authors present DeepSCFold, a pipeline which improves the protein complex structure prediction accuracy by capturing intrinsic and conserved protein-protein interaction patterns throu...
www.nature.com
November 19, 2025 at 3:03 PM
High-accuracy protein complex structure modeling based on sequence-derived structure complementarity
www.nature.com/articles/s41...
www.nature.com/articles/s41...