The @SabetiLab is excited to share our EID journal paper “Establishing methods to monitor H5N1 influenza virus in dairy cattle milk.” A collaboration led by @drelyse.bsky.social and a partnership between @broadinstitute.org , Mass DPH, Mass Dept. of Agr., & the CDC bit.ly/42gKYLo

Want a primer on the @sabetilab.bsky.social latest publication? See the thread I wrote on the preprint 👇

Now live at Emerging Infectious Diseases! "Establishing methods to monitor Influenza (A)H5N1 virus in dairy cattle milk, Massachusetts, USA" wwwnc.cdc.gov/eid/article/...

🐦‍⬛🔬Bird Flu (H5N1) – what’s the deal? 🤔
@pardissabeti.bsky.social joined @scishow.bsky.social
to break it all down—its origins, where it stands today, and why it matters. 🦠🐦 Here’s a sneak peek! 👀 Want the full story? Head to the SciShow's YouTube channel: youtu.be/5CyVi4UzKxE?... 🎥✨

What better way to kick off our Bluesky journey than with our annual holiday card? 🌟 Celebrate unity, humanity, and the milestones shaping a brighter future with us. Happy Holidays! 🎄✨ bit.ly/3Dsr6wk

😅

All cattle dairy farms remain NEGATIVE for the presence of H5N1 to date! We continue to test farms monthly so that if the outbreak should spread to the state we can detect it early and help to inform interventions. (6/FIN)

Validating all these methods allowed us to be in the position to support government mandated testing of all 95 dairy cattle farms in Massachusetts, led by MDAR and MDPH (5/n)

We also generated genomes (even from ultra-pasteurized milk!) from as little as 14 H5N1 cp/uL! Hybrid selection of RNASeq libraries resulted in the most complete genomes, but had a high input need (>500 cp/uL). Below that, we had success using an Amp-Seq protocol. (4/n)

With these samples, we were able to fully validate both dPCR and qPCR assays for H5N1 and RNAseP Bovine as a positive process control. (3/n)

With the current H5N1 outbreak in US dairy 🐄, we wanted to validate PCR and sequencing methods to deploy for surveillance efforts. Since there were no positives in NE, we sourced milk from states with active outbreaks. We screened 214 cartons in total! (2/n)

Excited to share our new preprint “Establishing methods to monitor H5N1 influenza virus in dairy cattle milk”. A HUGE collaborative effort between the Sabeti lab @broadinstitute.org and the Mass DPH and MDAR 🧵👇 (1/n)
medrxiv.org/cgi/content/...

Single mutation in H5N1 bird flu virus may make it more infectious to humans www.statnews.com/2024/12/05/h...

Guilty as charged 🙋🏼‍♀️ stay tuned for the preprint drop soon though 👀

If I have to call carjivirus by it's government name then fine but that guy will always be crAssphage in my heart

Can you add me please!

Here's a new starter pack for researchers at the Broad Institute of MIT and Harvard!

👋 blue sky! Interests include:

🦠Infectious diseases
🔎Diagnostics
🧫 Bacteriophages
💊 Antibiotic resistance
🏥 Public health
🌳Environmental engineering
💩 Wastewater