Eli Slenders
@elislenders.bsky.social
110 followers 29 following 10 posts
Molecular microscopy and spectroscopy, Istituto italiano di tecnologia
Posts Media Videos Starter Packs
Reposted by Eli Slenders
vicidominilab.bsky.social
Andrea Bucci and Laurent Le will run two workshops on building a custom #PhotonResolvedMicroscopy system and generating great #ImageScanningMicroscopy images.
Thanks @iitalk.bsky.social @erc.europa.eu @genoainstruments.bsky.social and #PNRR RNA&GeneTheraphy for support. See you at #Mifobio2025
elislenders.bsky.social
Great, Luciano! Thanks a lot for sharing your thoughts!
elislenders.bsky.social
Very happy to be in #ThePhotonics100 list for 2026. Big thanks to everyone in @vicidominilab.bsky.social and to all collaborators for their continuous support!
elislenders.bsky.social
@vicidominilab.bsky.social arrived in Berlin for the single molecule workshop #ws30. Looking forward to the talks and discussions! If you're interested in the use of #spad array detectors for imaging and SM localization, don't miss the poster of my colleague Francesco tonight and my talk on Friday!
elislenders.bsky.social
Thank you very much for your support, @vicidominilab.bsky.social! Looking forward to starting the project!
elislenders.bsky.social
Grateful to my colleagues at @vicidominilab.bsky.social @iitalk.bsky.social for their support in making this research possible, and to @erc.europa.eu and #MSCA for their support! 6/6
elislenders.bsky.social
ISM-FLUX offers more than just a simple experimental setup! Discover how the doughnut-shaped illumination provides a homogeneous localization uncertainty over a large FOV and learn about the self-calibrating procedure of ISM-FLUX. Check out our paper for all the details. 5/6
elislenders.bsky.social
We tested ISM-FLUX on DNA-origami nanorulers, and achieved a median localization uncertainty below 5 nm for single molecules. This precision effortlessly resolved 40 nm and 20 nm nanorulers. 4/6
elislenders.bsky.social
Similar to #MINFLUX, our localization uncertainty scales with L/sqrt(N), with L the TCP diameter and N the number of photons. However, while MINFLUX requires the emitter to be within the scanned area, ISM-FLUX has a much larger localization range, making it more robust. 3/6
elislenders.bsky.social
ISM-FLUX makes use of a #SPAD array detector. This boosts localization range and offers flexibility in the scanned target coordinate pattern (TCP). We chose a circular pattern, enabled by galvanometric scan mirrors found in most LSMs. 2/6
elislenders.bsky.social
Excited to share our paper on ISM-FLUX, our new #MINFLUX-based technology. Achieve sub-10 nm localization uncertainty on single molecules using a straightforward #confocal-based setup with an array detector. 1/6 www.nature.com/articles/s41...
Array detection enables large localization range for simple and robust MINFLUX - Light: Science & Applications
Light: Science & Applications - Array detection enables large localization range for simple and robust MINFLUX
www.nature.com
Reposted by Eli Slenders
zuninoale.bsky.social
Our last paper on s²ISM (super-resolution sectioning #ImageScanningMicroscopy) just got published on Nature Photonics @natphoton.nature.com‬! Discover how structured detection in laser scanning microscopes enhances both resolution and optical sectioning:

www.nature.com/articles/s41...

🧵1/7