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Reposted by Ian Monk

Conlon Lab @bconlon.bsky.social · 2d

Delighted to share our latest paper - A host-directed adjuvant sensitizes intracellular bacterial persisters to antibiotics. Excellently led by Dr. Kuan-Yi Lu. We think it's a great proof-of-concept that altering immune cell behavior can make antibiotics work better www.nature.com/articles/s41...

A host-directed adjuvant sensitizes intracellular bacterial persisters to antibiotics - Nature Microbiology

The authors developed a screen to find compounds that modulate intracellular Staphylococcus aureus metabolism and discovered KL1, which sensitizes persisters to antibiotics by reversing host-induced tolerance.

www.nature.com

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Reposted by Ian Monk

Angie Rasmussen @angierasmussen.bsky.social · 2d

I don’t know who needs to hear this but the CDC is being eviscerated right now. America is not going to have any kind of outbreak response capacity after tonight. Americans’ health data is no longer secure. Say goodbye to federal public health in any capacity. It’s a disaster. We won’t recover.

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Reposted by Ian Monk

Josh Mattila @joshmattila.bsky.social · 2d

Very cool.

www.nature.com/articles/s41...

A host-directed adjuvant sensitizes intracellular bacterial persisters to antibiotics - Nature Microbiology

The authors developed a screen to find compounds that modulate intracellular Staphylococcus aureus metabolism and discovered KL1, which sensitizes persisters to antibiotics by reversing host-induced tolerance.

www.nature.com

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Reposted by Ian Monk

Katherine Lemon @kathlemon.bsky.social · 11d

Happy to share this new article online at #mSphere journals.asm.org/doi/10.1128/...
Human nasal organoids (HNOs) are a new model system for studying bacterial colonization and interspecies interactions. A big congratulations to the whole team, especially co-first authors: Andrea Boyd and Leah Kafer

Nasal microbionts differentially colonize and elicit cytokines in human nasal epithelial organoids | mSphere

Human nasal microbiota often includes highly pathogenic members, many of which are antimicrobial resistance threats, e.g., methicillin-resistant Staphylococcus aureus and drug-resistant Streptococcus ...

journals.asm.org

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Reposted by Ian Monk

Contagion® @contagionlive.bsky.social · 5d

The center’s Chief Scientific Officer David Perlin, PhD, provides some insights on the evolution of their antiviral program from its foundation in treating COVID-19 to addressing all pancoronaviruses. #IDsky #Medsky

The Center for Discovery and Innovation: Harnessing the Cutting Edge of Medical Science

The center’s Chief Scientific Officer David Perlin, PhD, provides some insights on the evolution of their antiviral program from its foundation in treating COVID-19 to addressing all pancoronaviruses.

www.contagionlive.com

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Reposted by Ian Monk

Microbiology Society @microbiologysociety.org · 5d

Phosphate sensing by PhoPR regulates the cytotoxicity of Staphylococcus aureus. Published Open Access and fee-free in Microbiology using a Publish and Read agreement: doi.org/10.1099/mic.... #MicrobioJ #PublishAndRead

Fig. 1.
Cytotoxicity is altered in transposon mutants of TCSs in S. aureus. To quantify cytotoxicity, the bacterial supernatant from transposon mutants in each non-essential HK and RR gene was incubated with THP-1 cells, and the percentage of dead cells was quantified using trypan blue exclusion. THP-1 killing was calculated relative to the THP-1 killing of JE2 (WT) in each assay. Each dot represents one biological replicate (n = 3), error bars represent the sd and statistical significance was determined using multiple t-tests with an FDR of 1% applied. Significant hits, which had an adjusted P-value ≤0.01, are highlighted in purple.

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Reposted by Ian Monk

Saskia Popescu @saskiapopescu.bsky.social · 6d

The NIH ordered me to stop my ‘dangerous’ gain-of-function research. It isn’t dangerous at all www.statnews.com/2025/10/06/g... via @statnews.com

The NIH ordered me to stop my ‘dangerous’ gain-of-function research. It isn’t dangerous at all

Safe gain-of-function research is necessary to identify new treatments for diseases like for tuberculosis — but the NIH has imposed unfounded stops.

www.statnews.com

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Reposted by Ian Monk

Nature Reviews Disease Primers @natrevdiseaseprimers.nature.com · 8d

#Listeria monocytogenes causes serious infections by invading cells and crossing intestinal, blood–brain, and placental barriers, leading to #bacteraemia, CNS, and maternal–neonatal complications https://www.nature.c...

Schematics showing the mechanisms of Listeria monocytogenes infection and dissemination.

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Reposted by Ian Monk

Jörg Stülke @joergstuelke.bsky.social · 8d

Identification of genetic interactions in Bacillus subtilis cell division. Congratulations, Byoung-Mo Koo, Carol Gross, and all involved!
@cp-cellsystems.bsky.social #subtiwiki

www.cell.com/cell-systems...

Comprehensive genetic interaction analysis of the Bacillus subtilis envelope using double-CRISPRi

Koo et al. apply genome-scale double-CRISPRi to map cell envelope gene interactions in Bacillus subtilis, revealing >1,000 genetic interactions and uncovering gene networks in envelope biogenesis and ...

www.cell.com

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Reposted by Ian Monk

Jan-Willem Veening @veeninglab.com · 8d

Finally out, such an awesome piece of work, very proud of this one: www.cell.com/cell-systems... #MicroSky

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Reposted by Ian Monk

Prof Jenny Rohn @jennyrohn.bsky.social · 8d

This is pretty amazing. Aside from everything else, can finally start to scratch the surface of understanding compensatory pathways/redundancy in bacteria

Jan-Willem Veening @veeninglab.com · Aug 17

Genome-wide dual CRISPRi-seq! Knocking down every gene with every other gene in the pneumococcus, identifying > 4000 genetic interactions (GIs), most of which have never been reported. www.biorxiv.org/content/10.1...

Dual CRISPRi-Seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle

bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

www.biorxiv.org

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Reposted by Ian Monk

Dutchscientist (the real one) @dutchscientist.bsky.social · 10d

#LOL

If Chuck Norris was a Jedi caption.

Photo of Chuck Norris holding the lightsaber by the saber part.

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Reposted by Ian Monk

Jeremy Berg @jeremymberg.bsky.social · 13d

When I was at NIH and shutdowns loomed, it was discovered that the administration was planning to shut down PubMed. The head of NCBI called the powers that be and pointed out that this would threaten patient safety

Who knows what will happen now

Just in case, remember Euruope-PMC
(europepmc.org )

a cartoon of donald trump with the words be prepared above him

ALT: a cartoon of donald trump with the words be prepared above him

media.tenor.com

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Reposted by Ian Monk

Jeremy Berg @jeremymberg.bsky.social · 13d

This may just be paranoia on my part, but I have learned not to discount those feelings over the past 8 months.

Always good to be prepared in any case.

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Reposted by Ian Monk

Jason Yang @jasonyanglab.org · 12d

Very happy to share some more good news. Our collaboration with Jeffrey Boyd's lab is now published at J Inorganic Biochemistry! Here, Jeff found that iron limitation reprograms S. aureus metabolism towards fermentation. We're grateful to be part of this study.

www.sciencedirect.com/science/arti...

Fermentative growth decreases the iron demand of Staphylococcus aureus

Iron (Fe) is an essential nutrient for S. aureus survivability and pathogenesis, but excess Fe can catalyze the formation of toxic oxygen radicals, em…

www.sciencedirect.com

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Reposted by Ian Monk

Antimicrobial resistance #AMR @antimicrobial.bsky.social · 13d

Horizon Europe has launched a €253M AMR partnership to accelerate innovation and move potential therapies closer to market. The first funding call, focused on treatments, will open in November following a pre-announcement in mid-October.
sciencebusiness.net/news/r-d-fun...

Horizon Europe’s new antimicrobial resistance partnership looks to the market

Horizon Europe’s partnership for antimicrobial resistance (AMR) research launched last week with a plan to bring Europe closer to finding new anti-microbial therapies in the next decade. With more of ...

sciencebusiness.net

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Reposted by Ian Monk

Andy Edwards @bugsinblood.bsky.social · 13d

Now published! www.nature.com/articles/s41...

Very nice collaboration with Hoogenboom ( @ucl.ac.uk ) and Bonev ( @uniofnottingham.bsky.social ) labs.

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Dorottya Nagy @dotnagy.bsky.social · 14d

To summarise our recent pre-print: Autocycler, the automated consensus assembler, when used with Nanopore long-read only Enterobacterales assemblies, produces more complete chromosomes and plasmids, with an accuracy comparable to hybrid assemblies.

Figure 2 from my recently pre-printed manuscript on the completeness and accuracy of Nanopore long-read only bacterial genome assembly for Enterobacterales. a) tile plot of chromosome circularisation, with assembler on the x-axis and sample on the y-axis, shows that the consensus long-read only assembler, Autocycler, circularised more chromosomes at 95% (87/92) than any other long-read or hybrid assembler. b) complex upset plot of plasmid reconstruction, showing that the best plasmid reconstruction was achieved by long-read assemblers incorporating the separate plasmid assembly tool, Plassembler, namely Autocycler and Hybracter, reconstructing >96% of plasmids.

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Reposted by Ian Monk

Ryan Wick @rrwick.bsky.social · 14d

Happy to share that the paper describing Autocycler is now 100% up:
doi.org/10.1093/bioi...
(1/3)

Autocycler: long-read consensus assembly for bacterial genomes

AbstractMotivation. Long-read sequencing enables complete bacterial genome assemblies, but individual assemblers are imperfect and often produce sequence-l

doi.org

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Reposted by Ian Monk

ᐯIᑕTOᖇ ᑎIᘔET, ᗰᗪ @nizet.bsky.social · 16d

Pleased to share the new article from UCSD Skaggs School of Pharmacy PharmD/PhD student Daniel Sun focused on repurposing FDA-approved agents to inhibit virulence, restore antibiotic susceptibility, bolster host defenses, and modulate inflammation v. S. aureus
www.annualreviews.org/content/jour...

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Reposted by Ian Monk

Staph papers @staph-papers.bsky.social · 17d

Evolution under vancomycin selection drives divergent collateral sensitivity patterns in Staphylococcus aureus

Evolution under vancomycin selection drives divergent collateral sensitivity patterns in Staphylococcus aureus - PubMed

<span><i>Staphylococcus aureus</i> bacteremia is typically treated empirically with vancomycin, with therapy later tailored based on susceptibility results. However, these tests occur before vancomycin...

t.co

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Reposted by Ian Monk

George O'Toole (he/him/his) @geiselbiofilm.bsky.social · 17d

New in JB: Ma, Wang & Li review the role of DNA methylation in regulating pathogenesis, drug resistance, biofilm formation, adhesion, and motility and more!
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology

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Reposted by Ian Monk

🧬Jacob L Steenwyk @jlsteenwyk.bsky.social · 17d

Apple's approach to protein structure is great for accessibility - & potentially biological realism - reasons.

Eg, prediction could be achieved w/ smaller compute & the generative nature of prediction allows for multiple conformations

A summary here: genomely.substack.com/p/simplefold...

SimpleFold and the Future of Protein Folding

A Generative Shift in Protein Folding

genomely.substack.com

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Reposted by Ian Monk

Jeremy Barr @jeremyjbarr.bsky.social · 18d

Excited to annouce the latest research published by our lab, which is led by Dinesh Subedi and is out today in Nature Microbiology.

www.nature.com/articles/s41...

Rational design of a hospital-specific phage cocktail to treat Enterobacter cloacae complex infections - Nature Microbiology

The Entelli-02 phage product containing five phages has frontline potential to address infections caused by the multidrug-resistant Enterobacter cloacae complex.

www.nature.com

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Reposted by Ian Monk

Kumaran Ramamurthi @ramamurthilab.bsky.social · Sep 12

Immunization with peptides can elicit antigen-specific T cell responses, but they're not widely used because they are poorly immunogenic and are rapidly cleared. Domenico D'Atri solves this by using our "SSHEL" platform (synthetic bacterial spores) .
journals.asm.org/doi/10.1128/...

Immunization with peptide encapsulated within synthetic spores activates T cell responses and reduces tumor growth | mBio

Effective delivery of antigens to the immune system is essential for activating the adaptive immune system. Synthetic Spore Husk-Encased Lipids (SSHELs) are synthetic bacterial spore-like particles, w...

journals.asm.org

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