Jana Kroll
@janakroll.bsky.social
Postdoc at MDC Berlin
cryo-electron tomography, neurons, optogenetics
cryo-electron tomography, neurons, optogenetics
Pinned
Jana Kroll
@janakroll.bsky.social
· Dec 13
Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons - Nature Communications
Synaptic vesicle fusion is essential for neuronal communication, yet its nanoscale sequence has not been directly confirmed. Here, the authors use timed in situ cryo-electron tomography to visualize t...
www.nature.com
❄️🔬⏱️
I am excited to share that our study „Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons” has been published in Nature Communications www.nature.com/articles/s41...
👇 Short summary of our key findings (1/8)
I am excited to share that our study „Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons” has been published in Nature Communications www.nature.com/articles/s41...
👇 Short summary of our key findings (1/8)
Reposted by Jana Kroll
Please share🙏:
Our lab opens 2 PhD positions to investigate fungi's evolution: #Barcelona
Wet lab in Biochemistry/Structural Biology of cryo-tolerant fungi
www.upf.edu/documents/d/...
Computational in Physical Chemistry of cellular processes under extrem environments
www.upf.edu/documents/d/...
Our lab opens 2 PhD positions to investigate fungi's evolution: #Barcelona
Wet lab in Biochemistry/Structural Biology of cryo-tolerant fungi
www.upf.edu/documents/d/...
Computational in Physical Chemistry of cellular processes under extrem environments
www.upf.edu/documents/d/...
www.upf.edu
January 26, 2026 at 11:53 AM
Please share🙏:
Our lab opens 2 PhD positions to investigate fungi's evolution: #Barcelona
Wet lab in Biochemistry/Structural Biology of cryo-tolerant fungi
www.upf.edu/documents/d/...
Computational in Physical Chemistry of cellular processes under extrem environments
www.upf.edu/documents/d/...
Our lab opens 2 PhD positions to investigate fungi's evolution: #Barcelona
Wet lab in Biochemistry/Structural Biology of cryo-tolerant fungi
www.upf.edu/documents/d/...
Computational in Physical Chemistry of cellular processes under extrem environments
www.upf.edu/documents/d/...
Reposted by Jana Kroll
🔬🔦 Imaging spotlight on FocalPlane
@janakroll.bsky.social and colleagues highlight their research visualising synaptic vesicle fusion using cryo-ET combined with plunge freezing and optogenetics.
focalplane.biologists.com/2026/01/21/i...
@janakroll.bsky.social and colleagues highlight their research visualising synaptic vesicle fusion using cryo-ET combined with plunge freezing and optogenetics.
focalplane.biologists.com/2026/01/21/i...
Imaging spotlight: visualising synaptic vesicle fusion by cryo-ET - FocalPlane
Imaging spotlight: visualising synaptic vesicle fusion by cryo-ET - News
focalplane.biologists.com
January 22, 2026 at 1:47 PM
🔬🔦 Imaging spotlight on FocalPlane
@janakroll.bsky.social and colleagues highlight their research visualising synaptic vesicle fusion using cryo-ET combined with plunge freezing and optogenetics.
focalplane.biologists.com/2026/01/21/i...
@janakroll.bsky.social and colleagues highlight their research visualising synaptic vesicle fusion using cryo-ET combined with plunge freezing and optogenetics.
focalplane.biologists.com/2026/01/21/i...
❄️ With all the snow outside, I felt like sharing a cryo tomogram:
A neuronal synapse, captured by cryo-ET at the moment a neurotransmitter-filled vesicle fuses at the active zone.
Fusion was triggered optogenetically and arrested by plunge freezing.
More details: www.nature.com/articles/s41...
A neuronal synapse, captured by cryo-ET at the moment a neurotransmitter-filled vesicle fuses at the active zone.
Fusion was triggered optogenetically and arrested by plunge freezing.
More details: www.nature.com/articles/s41...
January 4, 2026 at 8:18 AM
❄️ With all the snow outside, I felt like sharing a cryo tomogram:
A neuronal synapse, captured by cryo-ET at the moment a neurotransmitter-filled vesicle fuses at the active zone.
Fusion was triggered optogenetically and arrested by plunge freezing.
More details: www.nature.com/articles/s41...
A neuronal synapse, captured by cryo-ET at the moment a neurotransmitter-filled vesicle fuses at the active zone.
Fusion was triggered optogenetically and arrested by plunge freezing.
More details: www.nature.com/articles/s41...
Reposted by Jana Kroll
Der Moment, in dem eine Nervenzelle ihre Neurotransmitter in den synaptischen Spalt ausschüttet, ist extrem kurz. Berliner Forschenden @bihatcharite.bsky.social @mdc-berlin.bsky.social konnten ihn mikroskopisch einfangen.
doi.org/10.1038/s414...
Bild: © Charité/Max Delbrück Center, Jana Kroll
doi.org/10.1038/s414...
Bild: © Charité/Max Delbrück Center, Jana Kroll
December 18, 2025 at 10:04 AM
Der Moment, in dem eine Nervenzelle ihre Neurotransmitter in den synaptischen Spalt ausschüttet, ist extrem kurz. Berliner Forschenden @bihatcharite.bsky.social @mdc-berlin.bsky.social konnten ihn mikroskopisch einfangen.
doi.org/10.1038/s414...
Bild: © Charité/Max Delbrück Center, Jana Kroll
doi.org/10.1038/s414...
Bild: © Charité/Max Delbrück Center, Jana Kroll
Reposted by Jana Kroll
A breathtaking glimpse into the #brain at work: NeuroCure researchers & colleagues @janakroll.bsky.social captured #synapses in action - revealing how #neurotransmitters are released in milliseconds 🧠 Published in NatureCommunications @Charite @mdc-berlin.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons - Nature Communications
Synaptic vesicle fusion is essential for neuronal communication, yet its nanoscale sequence has not been directly confirmed. Here, the authors use timed in situ cryo-electron tomography to visualize t...
www.nature.com
December 17, 2025 at 10:10 PM
A breathtaking glimpse into the #brain at work: NeuroCure researchers & colleagues @janakroll.bsky.social captured #synapses in action - revealing how #neurotransmitters are released in milliseconds 🧠 Published in NatureCommunications @Charite @mdc-berlin.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
It's been a fantastic experience to publish our work in @natcomms.nature.com and I sincerely thank the reviewers of our paper for their constructive and helpful comments! (8/8)
December 14, 2025 at 8:29 AM
It's been a fantastic experience to publish our work in @natcomms.nature.com and I sincerely thank the reviewers of our paper for their constructive and helpful comments! (8/8)
I deeply thank all co-authors for their constant support over the past years: @uljakravcenko.bsky.social, @mohsensadeghi.bsky.social, Christoph Diebolder, Malgorzata Lubas, Lia Ivanov, Thiemo Sprink, Magdalena Schacherl, @mishakudryashev.bsky.social and Christian Rosenmund. (7/8)
December 13, 2025 at 8:10 PM
I deeply thank all co-authors for their constant support over the past years: @uljakravcenko.bsky.social, @mohsensadeghi.bsky.social, Christoph Diebolder, Malgorzata Lubas, Lia Ivanov, Thiemo Sprink, Magdalena Schacherl, @mishakudryashev.bsky.social and Christian Rosenmund. (7/8)
Intriguingly, we observed filamentous connectors between the majority of fusing vesicles and at least one more adjacent vesicle. We suggest that these interconnectors physically couple vesicle fusion and direct vesicle resupply required for release site refilling. (6/8)
December 13, 2025 at 8:10 PM
Intriguingly, we observed filamentous connectors between the majority of fusing vesicles and at least one more adjacent vesicle. We suggest that these interconnectors physically couple vesicle fusion and direct vesicle resupply required for release site refilling. (6/8)
Our morphometric and computational analyses revealed that synaptic vesicle fusion is likely initiated via stalk formation, whereby vesicle and cell membrane form a tip-like connection. We did not find evidence for tight docking or strong membrane bending prior to fusion. (5/8)
December 13, 2025 at 8:10 PM
Our morphometric and computational analyses revealed that synaptic vesicle fusion is likely initiated via stalk formation, whereby vesicle and cell membrane form a tip-like connection. We did not find evidence for tight docking or strong membrane bending prior to fusion. (5/8)
In these stimulated synapses, we identified more than 50 synaptic vesicle fusion intermediates and characterized them morphometrically at nanoscale resolution and under near-native conditions. (4/8)
December 13, 2025 at 8:10 PM
In these stimulated synapses, we identified more than 50 synaptic vesicle fusion intermediates and characterized them morphometrically at nanoscale resolution and under near-native conditions. (4/8)
We confirmed successful optogenetic stimulation of synapses directly via cryo-confocal microscopy of the glutamate sensor iGluSnFR3. Neurotransmitters, released during exocytosis, induced an increase in fluorescence intensity that was preserved under cryo conditions. (3/8)
December 13, 2025 at 8:10 PM
We confirmed successful optogenetic stimulation of synapses directly via cryo-confocal microscopy of the glutamate sensor iGluSnFR3. Neurotransmitters, released during exocytosis, induced an increase in fluorescence intensity that was preserved under cryo conditions. (3/8)
We established a timed in situ cryo-electron tomography workflow to capture the complete sequence of synaptic vesicle fusion in optogenetically stimulated mouse neurons. Using a modified plunge freezer, the "Voptobot", we stimulated neurons only 2-3 milliseconds before cryofixation. (2/8)
December 13, 2025 at 8:10 PM
We established a timed in situ cryo-electron tomography workflow to capture the complete sequence of synaptic vesicle fusion in optogenetically stimulated mouse neurons. Using a modified plunge freezer, the "Voptobot", we stimulated neurons only 2-3 milliseconds before cryofixation. (2/8)
❄️🔬⏱️
I am excited to share that our study „Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons” has been published in Nature Communications www.nature.com/articles/s41...
👇 Short summary of our key findings (1/8)
I am excited to share that our study „Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons” has been published in Nature Communications www.nature.com/articles/s41...
👇 Short summary of our key findings (1/8)
Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons - Nature Communications
Synaptic vesicle fusion is essential for neuronal communication, yet its nanoscale sequence has not been directly confirmed. Here, the authors use timed in situ cryo-electron tomography to visualize t...
www.nature.com
December 13, 2025 at 8:10 PM
❄️🔬⏱️
I am excited to share that our study „Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons” has been published in Nature Communications www.nature.com/articles/s41...
👇 Short summary of our key findings (1/8)
I am excited to share that our study „Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons” has been published in Nature Communications www.nature.com/articles/s41...
👇 Short summary of our key findings (1/8)
Reposted by Jana Kroll
💥I am excited to present our first research paper from the lab about caveolae lipid trafficking 🎉 Led by my fantastic PhD candidate @estherocket.bsky.social
Here we looked into caveolae mediated dietary lipid uptake and how this process is mechanistically facilitated 👇 1/n
Here we looked into caveolae mediated dietary lipid uptake and how this process is mechanistically facilitated 👇 1/n
Lipid-induced Caveolin1-Lipid droplet trafficking is associated with lipid droplet growth https://www.biorxiv.org/content/10.64898/2025.12.10.693432v1
December 11, 2025 at 7:08 AM
💥I am excited to present our first research paper from the lab about caveolae lipid trafficking 🎉 Led by my fantastic PhD candidate @estherocket.bsky.social
Here we looked into caveolae mediated dietary lipid uptake and how this process is mechanistically facilitated 👇 1/n
Here we looked into caveolae mediated dietary lipid uptake and how this process is mechanistically facilitated 👇 1/n
Reposted by Jana Kroll
We got a large Helmholtz Bioengineering grant with Oliver Daumke, Artur Yakimovich, Alina Bazarova, and Dietrich Ruess to use protein design to target cancer.
Five open postdoctoral positions in Generative AI & Protein Engineering. Share & apply!
jobrxiv.org/job/max-delb...
Five open postdoctoral positions in Generative AI & Protein Engineering. Share & apply!
jobrxiv.org/job/max-delb...
Postdoctoral Positions in Protein Engineering
Post a job in 3min, or find thousands of job offers like this one at jobRxiv!
jobrxiv.org
November 21, 2025 at 7:08 AM
We got a large Helmholtz Bioengineering grant with Oliver Daumke, Artur Yakimovich, Alina Bazarova, and Dietrich Ruess to use protein design to target cancer.
Five open postdoctoral positions in Generative AI & Protein Engineering. Share & apply!
jobrxiv.org/job/max-delb...
Five open postdoctoral positions in Generative AI & Protein Engineering. Share & apply!
jobrxiv.org/job/max-delb...
Thank you so much! 😊
October 1, 2025 at 4:08 PM
Thank you so much! 😊
Reposted by Jana Kroll
Group Leader in Biomedical Engineering at the @mdc-berlin.bsky.social in Berlin.
It's a broad call for whoever is doing exciting Bioengineering, please apply or share. We have a great institute with amazing colleagues and core facilities,highly recommend
application.mdc-berlin.de/site/index.p...
It's a broad call for whoever is doing exciting Bioengineering, please apply or share. We have a great institute with amazing colleagues and core facilities,highly recommend
application.mdc-berlin.de/site/index.p...
MDC Berlin - Online Application System
application.mdc-berlin.de
October 1, 2025 at 3:39 PM
Group Leader in Biomedical Engineering at the @mdc-berlin.bsky.social in Berlin.
It's a broad call for whoever is doing exciting Bioengineering, please apply or share. We have a great institute with amazing colleagues and core facilities,highly recommend
application.mdc-berlin.de/site/index.p...
It's a broad call for whoever is doing exciting Bioengineering, please apply or share. We have a great institute with amazing colleagues and core facilities,highly recommend
application.mdc-berlin.de/site/index.p...
Reposted by Jana Kroll
Postdoc program at the MDC in Berlin, @mdc-berlin.bsky.social : take a look 👇👇
For this call, collaborative projects between two groups will be funded. Take a look at the call and the groups and drop me an email if interested. Share with colleagues too!
www.mdc-berlin.de/postdocs#t-g...
For this call, collaborative projects between two groups will be funded. Take a look at the call and the groups and drop me an email if interested. Share with colleagues too!
www.mdc-berlin.de/postdocs#t-g...
www.mdc-berlin.de
August 4, 2025 at 10:23 AM
Postdoc program at the MDC in Berlin, @mdc-berlin.bsky.social : take a look 👇👇
For this call, collaborative projects between two groups will be funded. Take a look at the call and the groups and drop me an email if interested. Share with colleagues too!
www.mdc-berlin.de/postdocs#t-g...
For this call, collaborative projects between two groups will be funded. Take a look at the call and the groups and drop me an email if interested. Share with colleagues too!
www.mdc-berlin.de/postdocs#t-g...
Reposted by Jana Kroll
🏆 Images from #mdcBerlin received top honors in the @helmholtzimaging.bsky.social “Best Scientific Image Contest”!
Jury Award for @janakroll.bsky.social, Daumke Lab
Public Choice Award for Dr. Rafael Deliz Aguirre, @coscialab.bsky.social
Full details: www.mdc-berlin.de/news/news/tw...
Jury Award for @janakroll.bsky.social, Daumke Lab
Public Choice Award for Dr. Rafael Deliz Aguirre, @coscialab.bsky.social
Full details: www.mdc-berlin.de/news/news/tw...
July 17, 2025 at 1:38 PM
🏆 Images from #mdcBerlin received top honors in the @helmholtzimaging.bsky.social “Best Scientific Image Contest”!
Jury Award for @janakroll.bsky.social, Daumke Lab
Public Choice Award for Dr. Rafael Deliz Aguirre, @coscialab.bsky.social
Full details: www.mdc-berlin.de/news/news/tw...
Jury Award for @janakroll.bsky.social, Daumke Lab
Public Choice Award for Dr. Rafael Deliz Aguirre, @coscialab.bsky.social
Full details: www.mdc-berlin.de/news/news/tw...
Reposted by Jana Kroll
We have a preprint for you
EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer
By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...
EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer
By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...
June 8, 2025 at 11:22 AM
We have a preprint for you
EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer
By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...
EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer
By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...
What an inspiring morning at the CatCat Symposium! Thank you so much for inviting me @gallegolab.bsky.social and @felixmendu.bsky.social, it's been a pleasure visiting you in Barcelona 😊
May 27, 2025 at 6:03 PM
What an inspiring morning at the CatCat Symposium! Thank you so much for inviting me @gallegolab.bsky.social and @felixmendu.bsky.social, it's been a pleasure visiting you in Barcelona 😊
In the left image, I see a queen with a crown🫅 But I guess that's not what you meant? 😅
May 13, 2025 at 5:46 PM
In the left image, I see a queen with a crown🫅 But I guess that's not what you meant? 😅
So many amazing images! 🤩 🤩🤩
Please make sure to vote, mine is also in the list 😎
Please make sure to vote, mine is also in the list 😎
🎉 Public Voting Now OPEN – #HelmholtzImaging Best Scientific Image Contest 2025
Choose your favorite(s) & cast your vote by May 30 👉 bit.ly/BSIC2025-Public-Voting
The award recipients will be announced at our conference on June 26.
#HIBestImage25 #HIConference25
@www.helmholtz.de
Choose your favorite(s) & cast your vote by May 30 👉 bit.ly/BSIC2025-Public-Voting
The award recipients will be announced at our conference on June 26.
#HIBestImage25 #HIConference25
@www.helmholtz.de
May 12, 2025 at 6:11 PM
So many amazing images! 🤩 🤩🤩
Please make sure to vote, mine is also in the list 😎
Please make sure to vote, mine is also in the list 😎
Reposted by Jana Kroll
A broad call for a PhD researcher/ postdoc in our group at the MDC in Berlin. An ideal PhD student - a computational scientist, a postdoc - a structural biologist, but feel free to re-convince me!
We extended the deadline for after Easter, Apr 24th. Apply here www.mdc-berlin.de/career/jobs/...
We extended the deadline for after Easter, Apr 24th. Apply here www.mdc-berlin.de/career/jobs/...
Scientific Researcher – Method Developments for In situ Structural Biology (F/M/D)
The Max Delbrück Center for Molecular Medicine in the Helmholtz Association aims to transform tomorrow’s medicine through our discoveries of today. At locations in Berlin-Buch, Berlin-Mitte, Heidelber...
www.mdc-berlin.de
April 8, 2025 at 11:19 AM
A broad call for a PhD researcher/ postdoc in our group at the MDC in Berlin. An ideal PhD student - a computational scientist, a postdoc - a structural biologist, but feel free to re-convince me!
We extended the deadline for after Easter, Apr 24th. Apply here www.mdc-berlin.de/career/jobs/...
We extended the deadline for after Easter, Apr 24th. Apply here www.mdc-berlin.de/career/jobs/...
Reposted by Jana Kroll
We are looking for a PhD student/Postdoc to develop methods of cryo-ET and/or study the structure and function of membrane proteins. Great team at the MDC in Berlin.
It's a broad call, please apply and share!
jobrxiv.org/job/max-delb...
It's a broad call, please apply and share!
jobrxiv.org/job/max-delb...
PhD /Postdoc in In Situ Structural Biology by Cryo-ET
Post a job in 3min, or find thousands of job offers like this one at jobRxiv!
jobrxiv.org
March 28, 2025 at 8:42 AM
We are looking for a PhD student/Postdoc to develop methods of cryo-ET and/or study the structure and function of membrane proteins. Great team at the MDC in Berlin.
It's a broad call, please apply and share!
jobrxiv.org/job/max-delb...
It's a broad call, please apply and share!
jobrxiv.org/job/max-delb...