Matthias Wilmanns
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matthiaswilmanns.bsky.social
Matthias Wilmanns
@matthiaswilmanns.bsky.social
120 followers 51 following 7 posts
EMBL Senior Scientist, Group Leader
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Reposted by Matthias Wilmanns
johnrubinstein.bsky.social
New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
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tinyurl.com/ukny4ptz
Cryo-EM maps and atomic models of the biotin-containing 3-methylcrotonyl-CoA carboxylase (MCC) complex and long-chain acyl-CoA carboxylase (LCC) complex
October 30, 2025 at 3:21 AM
matthiaswilmanns.bsky.social
We are delighted to share a stunning short/long chain acyl-CoA carboxylase complex with a total of 16 subunits (8 x AccA3, 2 x AccD4, 4 x AccD5, 2 x AccE5). It hosts two related carboxylate transferase subunits (AccD4, AccD5). Our story is now on www.biorxiv.org/content/10.1101/2025.11.15.688623v1
Architecture of an asymmetric mycobacterial short chain/long chain acyl-CoA carboxylase
Endogenous extraction has revealed a mycobacterial hybrid acyl-CoA carboxylase (ACCase) complex exhibiting distinct long-chain (LC) and short-chain (LC) acyl-CoA carboxyl transferase (CT) activities. ...
www.biorxiv.org
November 18, 2025 at 8:20 PM
matthiaswilmanns.bsky.social
We are excited to share our latest work, where we unravel the structural and functional secrets of the once-mysterious protein Pex8 revealing how it controls the peroxisomal cargo import receptor Pex5.

Read more here: www.biorxiv.org/content/10.1101/2025.08.30.673231v1
Ensemble model of the full length Pex5 receptor (different blue color) in complex with Pex8 (green colors). Key Findings: Pex8 binds to a novel site on the mostly unfolded N-terminal region of the Pex5 receptor. This interaction is essential for peroxisomal protein import. Computational modelling reveals the formation of an assembly with the trimeric peroxisomal E3-ubiquitin ligase. We propose that this action positions the Pex5 receptor for its recycling, a step essential for the entire protein import process. Why this matters: Peroxisomes rely entirely on the import of folded proteins to function. Impaired peroxisome function is linked to severe disorders, and recent data show their crucial roles in carcinogenesis and the immune response. Our work elevates Pex8 from an enigma to a central player in this critical biological process. Read the full story and see the structures here: https://www.biorxiv.org/content/10.1101/2025.08.30.673231v1 #CellBiology #StructuralBiology #Peroxisome #ProteinImport #bioRxiv #Biochemistry
September 3, 2025 at 10:28 AM
matthiaswilmanns.bsky.social
How is nitrogen built into metabolites? Mother nature has invented an amazing set of enzymes, called glutamine amidotransferases, by first generating ammonia and then building it into diverse metabolites. Here is our newest contribution, published in ACS Catalysis doi.org/10.1021/acsc....
Activity Regulation of a Glutamine Amidotransferase Bienzyme Complex by Substrate-Induced Subunit Interface Expansion
Glutamine amidotransferases are multienzyme machineries in which reactive ammonia is generated by a glutaminase and then transferred through a sequestered protein tunnel to a synthase active site for ...
doi.org
February 28, 2025 at 2:59 PM